FastQCFastQC Report
Fri 10 Feb 2017
ERR1630874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526412
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG88651.6840421570936834No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT83061.5778515687332355No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT74461.4144814327940851TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC45250.8595928664240178No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC31270.5940214128857245RNA PCR Primer, Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC21500.4084253398478758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18990.3607440559865656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.34155756327743286No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC11300.2146607600130696No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10930.2076320448621992No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG10440.1983237464191546No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT9330.1772376009665433No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9310.1768576704178476No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT8690.16507982340828098No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA8390.1593808651778455No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8330.1582410735317584No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8100.15387187222175785No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT7730.14684315707088744No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG7600.14437360850436542No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT7210.13696496280479928No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6860.13031617820262456No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6760.12841652545914606No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6650.12632690744131972No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6500.12347742832610198No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC5890.11188954659088318No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG5780.10979992857305684No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT5660.10752034528088265No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5630.1069504494578391No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC5390.10239128287349072No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC5300.10068159540436007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10350.033.067631
TTATACA11550.029.6320322
CGTCTTC4450.029.51685337
GGTATCA3200.028.906251
TATACAC12550.028.0079673
TCGCCGA405.934107E-527.751
GCCGAGC451.3218811E-424.6666683
CCGTCTT12200.024.56557537
GCCGTCT12450.024.07228936
GTATAGA952.8012437E-1023.3684221
TTACGGG400.001929804223.1253
GGTCTAA400.001929804223.1251
GTATCAA9450.022.5132271
TAGACTG450.003823093820.5555575
ATTACGG450.003823093820.5555572
TCGATGG450.003823093820.55555726
GATTACG450.003823093820.5555571
CGTAGGT555.138005E-420.18181827
TCTCGTC555.138005E-420.18181812
ATTGACG555.138005E-420.18181832
Produced by FastQC (version 0.11.5)