FastQCFastQC Report
Fri 10 Feb 2017
ERR1630873.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630873.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055853
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT129251.2241287376178311No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG95780.907133852913237No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT63520.6015988968161288TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC53670.5083093953419653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25480.2413214718336738No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC24560.23260813768583316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23350.2211482090783471No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC20500.19415581525079723RNA PCR Primer, Index 7 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14520.13751914328983295No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG13430.1271957365277174No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16500.031.5060621
TTATACA17700.029.4745792
TATACAC18850.028.3633963
CGTCTTC3000.027.13333337
CCGTCTT10150.026.9753737
GCCGTCT10400.026.32692336
GGTATCA6400.025.43751
TTACGGG1150.024.1304363
GATTACG1103.6379788E-1223.5454541
GTATCAA17350.022.2853031
TCGTGCG450.003825604420.55555737
TGCCGTC13600.020.40441135
ATGCCGT14150.019.48056634
TATATCG500.007034512718.4999985
ATACGCC500.007034512718.4999986
TATGCCG15150.018.19471733
TACACAT30350.017.6161465
ATACACA29750.017.5361354
GTACTGG7350.017.3673481
TTAACGG1201.0409349E-716.95833235
Produced by FastQC (version 0.11.5)