##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630873.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1055853
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49164419668268	31.0	31.0	34.0	28.0	34.0
2	31.476952757628194	31.0	31.0	34.0	28.0	34.0
3	30.947910362522055	31.0	31.0	34.0	27.0	34.0
4	35.10743825134749	37.0	35.0	37.0	32.0	37.0
5	35.30210928983485	37.0	35.0	37.0	33.0	37.0
6	35.48719092525191	37.0	35.0	37.0	33.0	37.0
7	35.50209735635548	37.0	35.0	37.0	33.0	37.0
8	35.51998715730315	37.0	35.0	37.0	33.0	37.0
9	37.28783552255854	39.0	37.0	39.0	34.0	39.0
10	37.116654496411904	39.0	37.0	39.0	33.0	39.0
11	37.18394606067322	39.0	37.0	39.0	33.0	39.0
12	37.113932526592244	39.0	37.0	39.0	33.0	39.0
13	37.181769621339335	39.0	37.0	39.0	33.0	39.0
14	38.33784721926253	40.0	38.0	41.0	34.0	41.0
15	38.35115967847797	40.0	38.0	41.0	34.0	41.0
16	38.349243692066985	40.0	38.0	41.0	34.0	41.0
17	38.307053159862214	40.0	38.0	41.0	33.0	41.0
18	38.349457737014525	40.0	38.0	41.0	34.0	41.0
19	38.373858861034634	40.0	38.0	41.0	34.0	41.0
20	38.369487987437644	40.0	38.0	41.0	34.0	41.0
21	38.33351517682859	40.0	38.0	41.0	34.0	41.0
22	38.2696909512972	40.0	38.0	41.0	34.0	41.0
23	38.272916779134974	40.0	38.0	41.0	34.0	41.0
24	38.22982081786006	40.0	38.0	41.0	34.0	41.0
25	38.20462602275127	40.0	38.0	41.0	34.0	41.0
26	38.11251850399629	40.0	37.0	41.0	33.0	41.0
27	38.01448591802078	40.0	37.0	41.0	33.0	41.0
28	38.010547869826574	40.0	37.0	41.0	33.0	41.0
29	37.95078292148623	40.0	37.0	41.0	33.0	41.0
30	37.87471362017251	40.0	37.0	41.0	33.0	41.0
31	37.86003544053955	40.0	37.0	41.0	33.0	41.0
32	37.77785828140849	40.0	37.0	41.0	33.0	41.0
33	37.756454733755554	40.0	37.0	41.0	33.0	41.0
34	37.69338913655594	40.0	37.0	41.0	33.0	41.0
35	37.66665814275282	40.0	37.0	41.0	33.0	41.0
36	37.635818622478695	40.0	37.0	41.0	33.0	41.0
37	37.56858956691888	40.0	37.0	41.0	32.0	41.0
38	37.52775528411625	40.0	37.0	41.0	32.0	41.0
39	37.45990208864302	40.0	37.0	41.0	32.0	41.0
40	37.3906064575277	39.0	37.0	41.0	32.0	41.0
41	37.33332102101334	39.0	36.0	41.0	31.0	41.0
42	37.27237503705535	39.0	36.0	41.0	31.0	41.0
43	36.64534646394905	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	17.0
12	9.0
13	7.0
14	5.0
15	10.0
16	10.0
17	7.0
18	16.0
19	40.0
20	79.0
21	163.0
22	335.0
23	671.0
24	1233.0
25	2203.0
26	3502.0
27	5541.0
28	8493.0
29	12277.0
30	16598.0
31	22383.0
32	29623.0
33	38679.0
34	51630.0
35	69983.0
36	97085.0
37	144047.0
38	254715.0
39	296482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.353584258414756	21.56635440728965	14.645694050213429	24.43436728408216
2	14.161535744085588	21.93875473195606	40.66513046797235	23.234579055986014
3	14.997258141048045	28.12721089015232	33.313065360424226	23.56246560837541
4	12.123941495643807	18.99289010875567	38.486607510704616	30.396560884895905
5	12.939869470466059	37.92118789263278	35.79371370825295	13.345228928648211
6	28.45329794961988	39.845224666691294	17.835247899092014	13.866229484596815
7	25.239119460758268	32.644316964577456	23.216678836921428	18.89988473774285
8	24.69036882975187	35.592549341622366	21.7057677536551	18.011314074970663
9	24.607876285808725	15.345128535885202	19.92985766010988	40.11713751819619
10	14.640769122216824	28.8524065376525	33.685939235859536	22.820885104271145
11	32.54477659295375	23.155117236963857	25.14677706082191	19.153329109260476
12	21.219052273375176	25.823196979124933	30.177780429662082	22.779970317837805
13	28.683254202999848	21.265176118266464	26.60559755950876	23.44597211922493
14	21.641554269391666	21.55205317406874	27.176794496961225	29.62959805957837
15	23.649883080315156	28.615536443046523	23.36168008235995	24.37290039427837
16	24.199486102705585	27.542849241324312	24.20867298762233	24.04899166834777
17	21.54589701407298	29.41574253234115	25.703104504130785	23.335255949455085
18	23.741562509175047	26.42204928148142	26.551896902314997	23.284491307028535
19	23.931361657351925	27.167986452659605	26.64168212809927	22.2589697618892
20	23.19868390770306	26.828924102124063	26.141044255213558	23.83134773495932
21	24.499812000344747	26.00210445961701	26.375262465513664	23.12282107452458
22	22.173730623486414	28.87381103240697	26.32525550431736	22.627202839789252
23	23.77414280207567	25.961947354413915	26.62889625733885	23.63501358617156
24	23.208154923081146	27.123472680382594	26.535038494942004	23.133333901594256
25	22.83026140949545	27.74780201410613	26.864629830099457	22.557306746298963
26	24.18783675379054	26.289265645880626	26.813675767365343	22.709221832963493
27	22.180360334251077	28.87589465579015	26.41362007779492	22.530124932163854
28	23.31849225223587	26.102591932778523	26.983206942633114	23.595708872352496
29	22.435414778382974	26.201374623171976	27.434121984783864	23.929088613661182
30	23.235715577831385	27.20672290555598	27.3472727737668	22.210288742845833
31	24.253755020822027	25.583675000213095	25.942058222119936	24.22051175684494
32	22.241921934208644	26.327907388623228	27.75698889902288	23.673181778145253
33	23.221414344610473	25.9080572769126	27.635381061568232	23.2351473169087
34	22.132436996437953	27.07147680595689	26.698981771136705	24.09710442646846
35	22.06093083033339	26.269755354201767	29.877738662484266	21.791575152980574
36	23.26876942150091	26.107138020160004	25.91762300244447	24.706469555894618
37	22.670864220682237	26.815664680594743	27.393017778042967	23.120453320680056
38	22.554465441685537	25.8303002406585	27.756894188869097	23.858340128786867
39	23.51908835794377	26.03013866513615	26.912079617143675	23.53869335977641
40	22.239175339749	25.762961321320297	29.728854300740725	22.269009038189974
41	22.930275331888055	26.075978379566095	26.55937900446369	24.43436728408216
42	22.89939982175549	25.834751617886205	28.29087003588568	22.97497852447263
43	23.29585652548224	25.203319022629096	28.343055330618938	23.157769121269723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	9.5
2	16.0
3	74.5
4	133.0
5	133.0
6	173.5
7	214.0
8	207.5
9	201.0
10	332.5
11	464.0
12	464.0
13	753.0
14	1042.0
15	2542.0
16	4042.0
17	4734.0
18	5426.0
19	5426.0
20	5786.5
21	6147.0
22	7574.5
23	9002.0
24	11749.0
25	14496.0
26	14496.0
27	18912.0
28	23328.0
29	28028.0
30	32728.0
31	38054.5
32	43381.0
33	43381.0
34	49636.0
35	55891.0
36	59872.5
37	63854.0
38	67824.0
39	71794.0
40	71794.0
41	74926.5
42	78059.0
43	78999.0
44	79939.0
45	80876.5
46	81814.0
47	81814.0
48	80692.5
49	79571.0
50	78158.5
51	76746.0
52	83951.0
53	91156.0
54	91156.0
55	75781.0
56	60406.0
57	59712.5
58	59019.0
59	50541.0
60	42063.0
61	42063.0
62	34493.0
63	26923.0
64	22650.0
65	18377.0
66	15168.0
67	11959.0
68	11959.0
69	9727.5
70	7496.0
71	6025.5
72	4555.0
73	3586.0
74	2617.0
75	2617.0
76	1981.5
77	1346.0
78	1084.0
79	822.0
80	635.5
81	449.0
82	449.0
83	329.0
84	209.0
85	155.5
86	102.0
87	70.5
88	39.0
89	39.0
90	28.0
91	17.0
92	12.0
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1055853.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.06659354254122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96984444360676	45.065400149810145
2	12.693005784138487	14.486932038331116
3	3.6366642956741093	6.22596129635719
4	1.5569613491728096	3.5540192189876536
5	0.7971147911493328	2.2744312896633314
6	0.48229039899090254	1.6513602101210323
7	0.31402111069789673	1.2544080561581952
8	0.2224401132354033	1.0155119623649285
9	0.18015784059306278	0.9252894836363623
>10	1.0138116078413613	11.059243834741661
>50	0.0909108830313272	3.539691833787422
>100	0.03894905657983986	3.702013374174059
>500	0.0021638360327708734	0.8377829747123783
>1k	9.98693553586557E-4	1.1573183405777345
>5k	4.993467767932785E-4	2.022932427715365
>10k+	1.664489255977595E-4	1.2277035088613935
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	12925	1.2241287376178311	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	9578	0.907133852913237	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	6352	0.6015988968161288	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	5367	0.5083093953419653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2548	0.2413214718336738	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	2456	0.23260813768583316	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2335	0.2211482090783471	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2050	0.19415581525079723	RNA PCR Primer, Index 7 (95% over 22bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1452	0.13751914328983295	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1343	0.1271957365277174	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	9.47101537808767E-5	0.0
5	0.0	0.0	0.0	9.47101537808767E-5	0.0
6	0.0	0.0	0.0	9.47101537808767E-5	0.0
7	0.0	0.0	0.0	9.47101537808767E-5	0.0
8	0.0	0.0	0.0	9.47101537808767E-5	0.0
9	0.0	0.0	0.0	2.8413046134263006E-4	0.0
10	0.0	0.0	0.0	2.8413046134263006E-4	0.0
11	0.0	0.0	0.0	2.8413046134263006E-4	0.0
12	0.0	0.0	0.0	3.788406151235068E-4	0.0
13	0.0	0.0	0.0	3.788406151235068E-4	0.0
14	0.0	0.0	0.0	3.788406151235068E-4	0.0
15	0.0	0.0	0.0	3.788406151235068E-4	0.0
16	0.0	0.0	0.0	4.7355076890438346E-4	0.0
17	0.0	0.0	0.0	5.682609226852601E-4	0.0
18	0.0	0.0	0.0	7.576812302470136E-4	0.0
19	0.0	0.0	0.0	7.576812302470136E-4	0.0
20	0.0	0.0	0.0	7.576812302470136E-4	0.0
21	0.0	0.0	0.0	0.0010418116915896435	0.0
22	0.0	0.0	0.0	0.0016100726142749039	0.0
23	0.0	0.0	0.0	0.0017994929218366571	0.0
24	0.0	0.0	0.0	0.0022730436907410405	0.0
25	0.0	0.0	0.0	0.0023677538445219175	0.0
26	0.0	0.0	0.0	0.002746594459645424	9.47101537808767E-5
27	0.0	0.0	0.0	0.0037884061512350677	9.47101537808767E-5
28	0.0	0.0	0.0	0.00947101537808767	9.47101537808767E-5
29	0.0	0.0	0.0	0.020741523678011995	9.47101537808767E-5
30	0.0	0.0	0.0	0.034853336591362624	9.47101537808767E-5
31	0.0	0.0	0.0	0.07851471748434678	9.47101537808767E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1650	0.0	31.506062	1
TTATACA	1770	0.0	29.474579	2
TATACAC	1885	0.0	28.363396	3
CGTCTTC	300	0.0	27.133333	37
CCGTCTT	1015	0.0	26.97537	37
GCCGTCT	1040	0.0	26.326923	36
GGTATCA	640	0.0	25.4375	1
TTACGGG	115	0.0	24.130436	3
GATTACG	110	3.6379788E-12	23.545454	1
GTATCAA	1735	0.0	22.285303	1
TCGTGCG	45	0.0038256044	20.555557	37
TGCCGTC	1360	0.0	20.404411	35
ATGCCGT	1415	0.0	19.480566	34
TATATCG	50	0.0070345127	18.499998	5
ATACGCC	50	0.0070345127	18.499998	6
TATGCCG	1515	0.0	18.194717	33
TACACAT	3035	0.0	17.616146	5
ATACACA	2975	0.0	17.536135	4
GTACTGG	735	0.0	17.367348	1
TTAACGG	120	1.0409349E-7	16.958332	35
>>END_MODULE