FastQCFastQC Report
Fri 10 Feb 2017
ERR1630872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943400
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT174841.8532965868136526No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT131761.3966504133983464RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG127091.3471486114055544No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC64300.6815772736909053No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC54290.5754716981132075RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC35640.3777824888700445RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20070.21274114903540386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.1824252702989188No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG15140.16048335806656774No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA13820.14649141403434385No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT10780.11426754292982828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG555.002221E-1033.6363641
CTTATAC22250.032.6764031
TTATACA25050.029.0978032
TATACAC25700.028.7937763
CGTCTTC6850.028.62773737
GCGTAAA405.93902E-527.7514
TCGCCGA358.8682136E-426.428571
CCGTCTT20800.025.17067337
GGTATCA3750.025.1599981
GGCGTAA451.3229664E-424.66666813
GCCGTCT21500.024.35116436
CGGCGTA502.7016047E-422.212
GTATCAA9350.021.9625661
TGCCGTC25250.020.73465335
CGTAAAT555.142155E-420.18181815
ATCGGCG555.142155E-420.18181810
ATGCCGT26500.019.82641434
CGTGGGA1355.638867E-1119.18518637
TATGCCG28550.018.532433
GTCGCCA701.2189036E-418.512
Produced by FastQC (version 0.11.5)