##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630872.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943400
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.538626245495017	31.0	31.0	34.0	30.0	34.0
2	31.548460886156455	31.0	31.0	34.0	30.0	34.0
3	31.03832838668645	31.0	31.0	34.0	27.0	34.0
4	35.16373436506254	37.0	35.0	37.0	32.0	37.0
5	35.347301250795	37.0	35.0	37.0	33.0	37.0
6	35.531754292982825	37.0	35.0	37.0	33.0	37.0
7	35.534237863048546	37.0	35.0	37.0	33.0	37.0
8	35.56205215179139	37.0	35.0	37.0	33.0	37.0
9	37.35216239135043	39.0	37.0	39.0	34.0	39.0
10	37.19193873224507	39.0	37.0	39.0	33.0	39.0
11	37.23588085647658	39.0	37.0	39.0	34.0	39.0
12	37.17682955268179	39.0	37.0	39.0	33.0	39.0
13	37.253597625609494	39.0	37.0	39.0	34.0	39.0
14	38.409102183591266	40.0	38.0	41.0	34.0	41.0
15	38.41552469790121	40.0	38.0	41.0	34.0	41.0
16	38.40967352130591	40.0	38.0	41.0	34.0	41.0
17	38.356980072079715	40.0	38.0	41.0	34.0	41.0
18	38.40473924104304	40.0	38.0	41.0	34.0	41.0
19	38.448325206699174	40.0	38.0	41.0	34.0	41.0
20	38.44884036463854	40.0	38.0	41.0	34.0	41.0
21	38.420309518761925	40.0	38.0	41.0	34.0	41.0
22	38.36098685605258	40.0	38.0	41.0	34.0	41.0
23	38.35494594021624	40.0	38.0	41.0	34.0	41.0
24	38.310126139495445	40.0	38.0	41.0	34.0	41.0
25	38.290893576425695	40.0	38.0	41.0	34.0	41.0
26	38.19933220267119	40.0	38.0	41.0	34.0	41.0
27	38.12184651261395	40.0	38.0	41.0	33.0	41.0
28	38.12756306974772	40.0	38.0	41.0	33.0	41.0
29	38.083379266482936	40.0	38.0	41.0	33.0	41.0
30	38.029624761500955	40.0	37.0	41.0	33.0	41.0
31	38.0099417002332	40.0	37.0	41.0	33.0	41.0
32	37.93595611617553	40.0	37.0	41.0	33.0	41.0
33	37.87937990248039	40.0	37.0	41.0	33.0	41.0
34	37.83622959508162	40.0	37.0	41.0	33.0	41.0
35	37.81119461522154	40.0	37.0	41.0	33.0	41.0
36	37.794626881492476	40.0	37.0	41.0	33.0	41.0
37	37.74133453466186	40.0	37.0	41.0	33.0	41.0
38	37.727958448166206	40.0	37.0	41.0	33.0	41.0
39	37.64245389018444	40.0	37.0	41.0	32.0	41.0
40	37.576451134195466	40.0	37.0	41.0	32.0	41.0
41	37.54050455798177	40.0	37.0	41.0	32.0	41.0
42	37.5036983252067	40.0	37.0	41.0	32.0	41.0
43	36.91022047911808	39.0	36.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	8.0
10	23.0
11	14.0
12	21.0
13	11.0
14	14.0
15	10.0
16	13.0
17	13.0
18	24.0
19	34.0
20	61.0
21	153.0
22	266.0
23	494.0
24	960.0
25	1754.0
26	2931.0
27	4535.0
28	6896.0
29	10212.0
30	14192.0
31	19205.0
32	25333.0
33	32919.0
34	44477.0
35	59451.0
36	83399.0
37	124979.0
38	224625.0
39	286372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.922196311214755	22.585435658257367	15.73500105999576	24.757366970532118
2	13.200339198643205	21.77019291922832	42.28185287258851	22.747615009539963
3	13.886686453254187	29.00752596989612	35.07398770404919	22.03179987280051
4	11.371316514733941	19.567521729913082	38.89060843756625	30.17055331778673
5	11.593491626033495	38.93979224083104	36.82753868984524	12.639177443290228
6	26.90958236167055	41.11288954844181	18.17871528513886	13.798812804748781
7	23.421560313758743	33.856900572397706	24.166525333898665	18.55501377994488
8	23.88371846512614	36.703625185499256	22.219525121899515	17.19313122747509
9	23.31121475514098	15.792770828916685	20.132711469154124	40.76330294678821
10	13.755776976892092	29.36675853296587	34.68496926012296	22.19249523001908
11	30.09243163027348	23.85806656773373	26.328492686029254	19.721009115963536
12	19.516535933856265	26.948590205639178	30.132075471698112	23.402798388806445
13	27.581301674793302	21.19429722281111	27.792134831460675	23.432266270934914
14	21.202777188891243	22.707759168963324	27.075683697265212	29.01377994488022
15	22.83241467034132	30.071549713801144	23.349162603349587	23.746873012507947
16	24.280686877252492	28.201717193131227	24.26690693237227	23.250688997244012
17	20.016111935552257	31.66323934704261	25.923998304006783	22.396650413398348
18	22.632923468306128	26.72662709349163	26.357006571973713	24.283442866228537
19	22.028301886792452	27.753444986220053	27.38668645325419	22.831566673733306
20	21.32181471274115	26.816408734365062	27.91700233199067	23.944774220903117
21	22.65433538265847	26.49332202671189	26.3561585753657	24.49618401526394
22	20.20797116811533	30.025757896968415	28.4564341742633	21.30983676065296
23	21.742209031163874	26.11363154547382	27.092537629849478	25.051621793512822
24	22.016005935976256	28.70998516005936	28.090523637905445	21.183485266058934
25	20.755776976892093	29.367288530845876	27.41562433750265	22.46131015475938
26	22.178291286834853	26.869514521941912	26.89071443714225	24.061479754080985
27	20.186241255034982	29.789166843332627	28.7750688997244	21.249523001907992
28	20.05077379690481	26.079393682425273	28.597413610345555	25.272418910324358
29	20.44424422302311	26.45049819800721	29.446894212423153	23.658363366546535
30	20.250476998092008	27.250052999788	29.731291074835703	22.768178927284293
31	21.223447106211577	26.17935128259487	28.165359338562645	24.43184227263091
32	21.48950604197583	28.25863896544414	29.500953996184016	20.750900996396016
33	20.443926224295105	26.759062963748143	28.689103243587027	24.107907568369725
34	20.73245707017172	28.738287046851813	29.106847572609713	21.42240831036676
35	21.16928132287471	28.05755776976892	30.426754292982828	20.346406614373542
36	22.47996608013568	26.903646385414458	25.51791392834429	25.098473606105575
37	21.337714649141404	27.574093703625184	28.418910324358706	22.66928132287471
38	21.85796056815773	26.248463006147976	28.81057875768497	23.082997668009327
39	23.055861776552895	26.692919228323085	27.482934068263727	22.768284926860293
40	20.65730337078652	25.4436082255671	32.132393470426116	21.76669493322027
41	21.349162603349587	27.033283866864533	27.236061055755776	24.381492474030104
42	22.15051939792241	25.9561161755353	30.209773160907353	21.683591265634938
43	21.808882764468944	25.231397074411703	30.760652957388167	22.199067203731186
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	17.5
2	24.0
3	85.5
4	147.0
5	147.0
6	207.0
7	267.0
8	264.5
9	262.0
10	369.5
11	477.0
12	477.0
13	766.0
14	1055.0
15	2322.0
16	3589.0
17	4376.5
18	5164.0
19	5164.0
20	6161.0
21	7158.0
22	9473.0
23	11788.0
24	15329.0
25	18870.0
26	18870.0
27	23481.5
28	28093.0
29	34085.0
30	40077.0
31	44424.0
32	48771.0
33	48771.0
34	53062.5
35	57354.0
36	60468.0
37	63582.0
38	67653.5
39	71725.0
40	71725.0
41	73074.5
42	74424.0
43	71946.5
44	69469.0
45	68138.5
46	66808.0
47	66808.0
48	64860.5
49	62913.0
50	74873.5
51	86834.0
52	68683.5
53	50533.0
54	50533.0
55	56137.5
56	61742.0
57	51612.0
58	41482.0
59	31623.0
60	21764.0
61	21764.0
62	19649.0
63	17534.0
64	14093.5
65	10653.0
66	8584.5
67	6516.0
68	6516.0
69	5419.5
70	4323.0
71	3695.5
72	3068.0
73	3224.5
74	3381.0
75	3381.0
76	2826.0
77	2271.0
78	1610.0
79	949.0
80	574.5
81	200.0
82	200.0
83	140.0
84	80.0
85	55.0
86	30.0
87	19.5
88	9.0
89	9.0
90	6.0
91	3.0
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	943400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.067176808845346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.63829944689888	38.58559342907913
2	12.213470590152326	11.98561041793274
3	3.7263739038755532	5.485279415919872
4	1.6189816726859283	3.177554399358425
5	0.8403055081626948	2.0615709471232795
6	0.553817013337391	1.6304542387903453
7	0.35361614061754976	1.2145661985899983
8	0.26717162323653737	1.0487485820522715
9	0.21336775740364128	0.9422418130048158
>10	1.3126922303248851	12.806530520975723
>50	0.17034744933323223	5.8430366520671715
>100	0.087444189710518	7.608344657299443
>500	0.0017315681098340838	0.5485433927227882
>1k	0.0012986760823755629	1.1964936810096674
>5k	4.3289202745852096E-4	1.259472622323242
>10k+	6.493380411877814E-4	4.605959031751133
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	17484	1.8532965868136526	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	13176	1.3966504133983464	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	12709	1.3471486114055544	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	6430	0.6815772736909053	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	5429	0.5754716981132075	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3564	0.3777824888700445	RNA PCR Primer, Index 39 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2007	0.21274114903540386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1721	0.1824252702989188	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1514	0.16048335806656774	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1382	0.14649141403434385	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	1078	0.11426754292982828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0599957600169599E-4	0.0
5	1.0599957600169599E-4	0.0	0.0	1.0599957600169599E-4	0.0
6	1.0599957600169599E-4	0.0	0.0	1.0599957600169599E-4	0.0
7	1.0599957600169599E-4	0.0	0.0	1.0599957600169599E-4	0.0
8	2.1199915200339198E-4	0.0	0.0	1.0599957600169599E-4	0.0
9	2.1199915200339198E-4	0.0	0.0	2.1199915200339198E-4	0.0
10	5.2999788000848E-4	0.0	0.0	2.1199915200339198E-4	0.0
11	6.359974560101759E-4	0.0	0.0	2.1199915200339198E-4	0.0
12	6.359974560101759E-4	0.0	0.0	2.1199915200339198E-4	0.0
13	6.359974560101759E-4	0.0	0.0	3.1799872800508796E-4	0.0
14	6.359974560101759E-4	0.0	0.0	3.1799872800508796E-4	0.0
15	9.539961840152639E-4	0.0	0.0	3.1799872800508796E-4	0.0
16	9.539961840152639E-4	0.0	0.0	4.2399830400678397E-4	0.0
17	9.539961840152639E-4	0.0	0.0	5.2999788000848E-4	0.0
18	9.539961840152639E-4	0.0	0.0	6.359974560101759E-4	0.0
19	0.00105999576001696	0.0	0.0	8.479966080135679E-4	0.0
20	0.00105999576001696	0.0	0.0	8.479966080135679E-4	0.0
21	0.0012719949120203519	0.0	0.0	9.539961840152639E-4	0.0
22	0.001483994064023744	0.0	0.0	0.001483994064023744	0.0
23	0.001483994064023744	0.0	0.0	0.001801992792028832	0.0
24	0.001483994064023744	0.0	0.0	0.00211999152003392	0.0
25	0.001483994064023744	0.0	0.0	0.00211999152003392	0.0
26	0.001483994064023744	0.0	0.0	0.002331990672037312	0.0
27	0.001483994064023744	0.0	0.0	0.0034979860080559676	1.0599957600169599E-4
28	0.0015899936400254398	0.0	0.0	0.0067839728641085435	1.0599957600169599E-4
29	0.0015899936400254398	0.0	0.0	0.012931948272206912	1.0599957600169599E-4
30	0.001801992792028832	0.0	0.0	0.024167903328386687	1.0599957600169599E-4
31	0.001801992792028832	0.0	0.0	0.06137375450498198	1.0599957600169599E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	55	5.002221E-10	33.636364	1
CTTATAC	2225	0.0	32.676403	1
TTATACA	2505	0.0	29.097803	2
TATACAC	2570	0.0	28.793776	3
CGTCTTC	685	0.0	28.627737	37
GCGTAAA	40	5.93902E-5	27.75	14
TCGCCGA	35	8.8682136E-4	26.42857	1
CCGTCTT	2080	0.0	25.170673	37
GGTATCA	375	0.0	25.159998	1
GGCGTAA	45	1.3229664E-4	24.666668	13
GCCGTCT	2150	0.0	24.351164	36
CGGCGTA	50	2.7016047E-4	22.2	12
GTATCAA	935	0.0	21.962566	1
TGCCGTC	2525	0.0	20.734653	35
CGTAAAT	55	5.142155E-4	20.181818	15
ATCGGCG	55	5.142155E-4	20.181818	10
ATGCCGT	2650	0.0	19.826414	34
CGTGGGA	135	5.638867E-11	19.185186	37
TATGCCG	2855	0.0	18.5324	33
GTCGCCA	70	1.2189036E-4	18.5	12
>>END_MODULE