FastQCFastQC Report
Fri 10 Feb 2017
ERR1630871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109836
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT1424812.972067446010415No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG1225711.15936487126261No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT82477.508467169234131RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC66966.096361848574238No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC32742.98080774973597No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC29362.6730762227320732RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG15331.3957172511744782No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA11251.0242543428384137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9930.9040751666120398No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT9720.8849557522123894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.7920900251283731No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT7170.652791434502349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3820.347791252412688No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2800.25492552532867185No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT2520.22943297279580466No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2380.2166866965293711No Hit
CTTTACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA2050.1866419024727776No Hit
CTGATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT1850.16843293637787246No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1780.16205979824465566No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1480.134746349102298No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1350.12291052114060963No Hit
CTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA1280.11653738300739283No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.11562693470264758No Hit
TCGCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT1270.11562693470264758RNA PCR Primer, Index 47 (95% over 22bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1250.11380603809315708No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1240.11289558978841183No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1220.1110746931789213No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1190.10834334826468554No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG1100.10014931352197821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT200.00183469637.04
CAATATA200.00183469637.012
CTTAATA200.00183469637.01
ATACTTC200.00183469637.06
AATATAA200.00183469637.013
TACTTCA200.00183469637.07
TCTACAC251.2263679E-436.9999963
TTCTACA251.2263679E-436.9999962
TTTACAC251.2263679E-436.9999962
TTTATAC251.2263679E-436.9999963
CTTCTAC251.2263679E-436.9999961
CTTATAC16250.036.5446171
TTATACA17200.034.4186062
CGTCTTC3650.032.94520637
TATACAC18250.032.5397263
GGTATCA1350.031.5185181
TATACTT250.005474987429.5999985
TGTCTCT250.005474987429.59999837
GGGGGGG250.005474987429.59999818
CGGCCGC250.005474987429.59999821
Produced by FastQC (version 0.11.5)