##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630871.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109836
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.041307039586293	31.0	30.0	34.0	28.0	34.0
2	31.220965803561676	31.0	31.0	34.0	28.0	34.0
3	30.685831603481553	31.0	31.0	34.0	26.0	34.0
4	34.75525328671838	35.0	35.0	37.0	32.0	37.0
5	34.952957136093815	35.0	35.0	37.0	32.0	37.0
6	35.13799664955024	36.0	35.0	37.0	32.0	37.0
7	35.07203466987144	36.0	35.0	37.0	32.0	37.0
8	35.13633052915255	37.0	35.0	37.0	32.0	37.0
9	36.98724461925052	39.0	37.0	39.0	33.0	39.0
10	36.7632197093849	39.0	37.0	39.0	32.0	39.0
11	36.759441348920205	39.0	35.0	39.0	32.0	39.0
12	36.593466622965146	39.0	35.0	39.0	32.0	39.0
13	36.78169270548818	39.0	37.0	39.0	32.0	39.0
14	37.44012891947995	39.0	36.0	40.0	32.0	41.0
15	37.51024254342838	39.0	36.0	40.0	32.0	41.0
16	37.48588805127645	39.0	36.0	40.0	32.0	41.0
17	37.36524454641466	39.0	36.0	40.0	32.0	41.0
18	37.65205397137551	39.0	37.0	41.0	32.0	41.0
19	37.4932080556466	39.0	36.0	40.0	32.0	41.0
20	37.72502640300084	39.0	37.0	41.0	32.0	41.0
21	37.54818092428712	39.0	37.0	41.0	32.0	41.0
22	37.09498707163407	39.0	36.0	40.0	31.0	41.0
23	37.509523289267634	39.0	36.0	40.0	32.0	41.0
24	37.13288903456062	39.0	36.0	40.0	31.0	41.0
25	37.133143960085945	39.0	36.0	40.0	31.0	41.0
26	36.98352088568411	39.0	36.0	40.0	31.0	41.0
27	36.6209712662515	39.0	35.0	40.0	30.0	41.0
28	36.937461305947046	39.0	35.0	40.0	31.0	41.0
29	36.595260206125495	38.0	35.0	40.0	30.0	41.0
30	36.72488073127208	39.0	35.0	40.0	30.0	41.0
31	36.62592410502932	39.0	35.0	40.0	30.0	41.0
32	36.04161659200991	38.0	35.0	40.0	30.0	41.0
33	36.19677519210459	38.0	35.0	40.0	30.0	41.0
34	35.993217160129646	38.0	35.0	40.0	30.0	41.0
35	36.12306529735242	38.0	35.0	40.0	30.0	41.0
36	36.28482464765651	38.0	35.0	40.0	30.0	41.0
37	35.95944863250665	38.0	34.0	40.0	28.0	41.0
38	35.99836119305146	38.0	35.0	40.0	28.0	41.0
39	35.9024181506974	38.0	34.0	40.0	28.0	41.0
40	35.496868057831676	38.0	34.0	40.0	26.0	41.0
41	35.56940347427073	38.0	34.0	40.0	27.0	41.0
42	35.43431115481263	38.0	34.0	40.0	26.0	41.0
43	34.22548162715321	37.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	10.0
12	7.0
13	12.0
14	11.0
15	8.0
16	7.0
17	7.0
18	12.0
19	11.0
20	22.0
21	46.0
22	82.0
23	177.0
24	298.0
25	515.0
26	817.0
27	1232.0
28	1684.0
29	2312.0
30	2997.0
31	3702.0
32	4584.0
33	5668.0
34	7217.0
35	9295.0
36	12158.0
37	16428.0
38	23508.0
39	16997.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.291744054772572	28.55712152663972	17.78469718489384	32.36643723369387
2	7.852616628427837	17.76193597727521	49.05677555628392	25.32867183801304
3	9.421319057503915	33.794020175534435	42.21930878764704	14.565351979314615
4	5.94431698168178	25.94777668523981	20.582504825376017	47.52540150770239
5	9.661677409956662	38.261590006919405	34.03619942459667	18.040533158527257
6	21.107833497214028	37.69164936814888	14.997814924068612	26.202702210568486
7	15.158053825703776	36.898648894715755	11.87315634218289	36.070140937397575
8	24.960850722895955	29.7698386685604	27.80782257183437	17.461488036709273
9	14.059142721876253	10.804290032411961	10.619469026548673	64.51709821916312
10	13.195127280673002	28.435121453803852	27.148657999198804	31.22109326632434
11	23.937506828362284	15.775337776321061	26.093448413999056	34.193706981317604
12	13.419097563640337	27.64485232528497	14.989620889325906	43.94642922174879
13	37.228231181033536	11.330529152554718	25.626388433664737	25.814851232747003
14	21.020430459958483	27.924359954841766	14.605411704723407	36.449797880476346
15	28.820241086711096	22.993371936341454	12.01154448450417	36.174842492443275
16	36.530827779598674	18.46298117192906	12.058887796350923	32.947303252121344
17	13.734112677082194	43.89180232346408	12.379365599621254	29.994719399832476
18	30.97071998251939	14.892202920718162	13.723187297425252	40.41388979933719
19	20.874758731199243	35.45467788338978	17.192905786809426	26.47765759860155
20	17.284861065588697	18.625040970173714	22.089296769729412	42.00080119450818
21	38.539276739866715	17.49881641720383	14.313157798900178	29.648749044029284
22	15.303725554463018	44.915146217997744	17.492443279070613	22.288684948468624
23	35.86802141374413	14.146545759131795	15.413889799337193	34.57154302778688
24	26.961105648421285	33.946065042426895	16.288830620197388	22.80399868895444
25	19.8659820095415	35.75057358243199	21.219818638697692	23.163625769328817
26	40.37291962562365	28.681852944389817	14.793874503805673	16.151352926180852
27	13.126843657817108	37.666156815616006	29.690629666047563	19.51636986051932
28	32.309078990494925	14.808441676681596	23.662551440329217	29.219927892494262
29	13.102261553588987	30.57376452165046	18.272697476237298	38.051276448523254
30	26.102552897046504	19.10939946829819	39.77930733092975	15.008740303725554
31	37.13627590225427	12.565097053789287	15.045158235915364	35.25346880804108
32	20.232892676353835	30.307913616664845	22.063804217196548	27.395389489784773
33	37.471320878400526	15.551367493353727	29.883644706653556	17.09366692159219
34	14.925889507993736	32.89631814705561	19.158563676754433	33.01922866819622
35	15.579591390800832	27.614807531228376	44.04384719035653	12.76175388761426
36	34.68625951418478	16.40718889981427	16.84511453439674	32.06143705160421
37	18.492115517680908	27.08401616956189	31.765541352562	22.6583269601952
38	17.187443096980953	13.408172183983394	36.09108124840672	33.31330347062894
39	31.724571178848464	17.746458356094543	20.745475071925416	29.783495393131577
40	13.682217123711716	12.476783568228996	54.26362941112204	19.577369896937252
41	23.042536144797698	22.96878983211333	18.770712698932954	35.217961324156015
42	25.504388360828873	13.460978185658618	41.19778578972286	19.83684766378965
43	24.703193852653047	13.09224662223679	37.68254488510143	24.52201464000874
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	17.0
4	31.0
5	31.0
6	47.5
7	64.0
8	63.0
9	62.0
10	107.0
11	152.0
12	152.0
13	266.5
14	381.0
15	858.0
16	1335.0
17	1425.5
18	1516.0
19	1516.0
20	1213.5
21	911.0
22	730.5
23	550.0
24	611.0
25	672.0
26	672.0
27	788.0
28	904.0
29	868.5
30	833.0
31	932.5
32	1032.0
33	1032.0
34	1162.5
35	1293.0
36	1376.0
37	1459.0
38	1533.5
39	1608.0
40	1608.0
41	1799.5
42	1991.0
43	2260.5
44	2530.0
45	3120.0
46	3710.0
47	3710.0
48	4445.0
49	5180.0
50	6719.5
51	8259.0
52	20894.0
53	33529.0
54	33529.0
55	20008.5
56	6488.0
57	13380.5
58	20273.0
59	15600.5
60	10928.0
61	10928.0
62	6418.5
63	1909.0
64	1462.0
65	1015.0
66	812.0
67	609.0
68	609.0
69	465.0
70	321.0
71	238.0
72	155.0
73	110.0
74	65.0
75	65.0
76	52.5
77	40.0
78	27.5
79	15.0
80	11.0
81	7.0
82	7.0
83	5.0
84	3.0
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	109836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.731818347354235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00513811426815	15.135292618085144
2	10.79443151376751	4.475763866127681
3	4.68578455052479	2.9143450234895663
4	2.6437134952351675	2.192359517826578
5	1.7741864652408765	1.8391055755854182
6	1.11984541741689	1.3929859062602425
7	0.8783101313073647	1.2746276266433592
8	0.6762988011066707	1.1216723114461562
9	0.5665100346932502	1.0570304818092429
>10	3.5220236265425324	13.8005754033286
>50	0.19322822888762023	2.746822535416439
>100	0.08783101313073646	3.005389853964092
>500	0.017566202626147293	3.2339123784551513
>1k	0.017566202626147293	8.073855566480935
>5k	0.008783101313073647	13.604829017808367
>10k+	0.008783101313073647	24.131432317273024
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	14248	12.972067446010415	No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	12257	11.15936487126261	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	8247	7.508467169234131	RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	6696	6.096361848574238	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	3274	2.98080774973597	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	2936	2.6730762227320732	RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1533	1.3957172511744782	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	1125	1.0242543428384137	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	993	0.9040751666120398	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	972	0.8849557522123894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	870	0.7920900251283731	No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	717	0.652791434502349	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	382	0.347791252412688	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	280	0.25492552532867185	No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT	252	0.22943297279580466	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	238	0.2166866965293711	No Hit
CTTTACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	205	0.1866419024727776	No Hit
CTGATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	185	0.16843293637787246	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	178	0.16205979824465566	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	148	0.134746349102298	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	135	0.12291052114060963	No Hit
CTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	128	0.11653738300739283	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.11562693470264758	No Hit
TCGCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	127	0.11562693470264758	RNA PCR Primer, Index 47 (95% over 22bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	125	0.11380603809315708	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	124	0.11289558978841183	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	122	0.1110746931789213	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	119	0.10834334826468554	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	110	0.10014931352197821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.104483047452566E-4	0.0
4	0.0	0.0	0.0	9.104483047452566E-4	0.0
5	0.0	0.0	0.0	9.104483047452566E-4	0.0
6	0.0	0.0	0.0	9.104483047452566E-4	0.0
7	0.0	0.0	0.0	9.104483047452566E-4	0.0
8	0.0	0.0	0.0	9.104483047452566E-4	0.0
9	0.0	0.0	0.0	9.104483047452566E-4	0.0
10	0.0	0.0	0.0	9.104483047452566E-4	0.0
11	0.0	0.0	0.0	9.104483047452566E-4	0.0
12	0.0	0.0	0.0	9.104483047452566E-4	0.0
13	0.0	0.0	0.0	9.104483047452566E-4	0.0
14	0.0	0.0	0.0	9.104483047452566E-4	0.0
15	0.0	0.0	0.0	9.104483047452566E-4	0.0
16	0.0	0.0	0.0	9.104483047452566E-4	0.0
17	0.0	0.0	0.0	9.104483047452566E-4	0.0
18	0.0	0.0	0.0	9.104483047452566E-4	0.0
19	0.0	0.0	0.0	0.0027313449142357697	0.0
20	0.0	0.0	0.0	0.0027313449142357697	0.0
21	0.0	0.0	0.0	0.0027313449142357697	0.0
22	0.0	0.0	0.0	0.0036417932189810264	0.0
23	0.0	0.0	0.0	0.0036417932189810264	0.0
24	0.0	0.0	0.0	0.00819403474270731	0.0
25	0.0	0.0	0.0	0.00819403474270731	0.0
26	0.0	0.0	0.0	0.00819403474270731	0.0
27	0.0	0.0	0.0	0.010925379656943079	0.0
28	0.0	0.0	0.0	0.017298517790159874	0.0
29	0.0	0.0	0.0	0.04643286354200808	0.0
30	0.0	0.0	0.0	0.06555227794165848	0.0
31	0.0	0.0	0.0	0.10470155504570451	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACT	20	0.001834696	37.0	4
CAATATA	20	0.001834696	37.0	12
CTTAATA	20	0.001834696	37.0	1
ATACTTC	20	0.001834696	37.0	6
AATATAA	20	0.001834696	37.0	13
TACTTCA	20	0.001834696	37.0	7
TCTACAC	25	1.2263679E-4	36.999996	3
TTCTACA	25	1.2263679E-4	36.999996	2
TTTACAC	25	1.2263679E-4	36.999996	2
TTTATAC	25	1.2263679E-4	36.999996	3
CTTCTAC	25	1.2263679E-4	36.999996	1
CTTATAC	1625	0.0	36.544617	1
TTATACA	1720	0.0	34.418606	2
CGTCTTC	365	0.0	32.945206	37
TATACAC	1825	0.0	32.539726	3
GGTATCA	135	0.0	31.518518	1
TATACTT	25	0.0054749874	29.599998	5
TGTCTCT	25	0.0054749874	29.599998	37
GGGGGGG	25	0.0054749874	29.599998	18
CGGCCGC	25	0.0054749874	29.599998	21
>>END_MODULE