FastQCFastQC Report
Fri 10 Feb 2017
ERR1630870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753163
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT140711.8682542822735584No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG115071.5278233264246917No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT98431.306888415920591TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC60350.8012873707285142No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC36470.4842245304137352RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC30680.4073487412419357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25340.3364477543373745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.3225065490471518No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG13780.1829617227612084No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12110.16078856768056848No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12100.16065579429685206No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC9620.12772799513518324No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT9470.12573639437943712No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9350.12414311377484025No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT7660.1017044119267675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17750.032.935211
TTATACA18950.030.4591032
CGTCTTC5250.030.30476437
GATTACG551.853914E-830.2727281
CGACGAG250.00549515529.624
TATACAC19900.029.5628153
GGTATCA4750.024.9263151
CCGTCTT15000.024.66666637
GCCGTCT15200.024.34210636
GTATCAA12900.023.9496121
CTGTCCG450.003824601920.5555559
AGGACCG656.898092E-519.9230775
CGATTAG851.2441415E-619.58823616
TTACGGG851.2441415E-619.5882363
TGCCGTC19050.019.51968635
CGTGAAA500.007032685418.510
TGAGTAC500.007032685418.534
CGGTGTA902.1502128E-618.534
CGGCTTA701.2184889E-418.528
TCGGTGT902.1502128E-618.533
Produced by FastQC (version 0.11.5)