##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630870.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	753163
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341734790476963	31.0	31.0	34.0	28.0	34.0
2	31.336114493144247	31.0	31.0	34.0	28.0	34.0
3	30.770288768832245	31.0	31.0	34.0	26.0	34.0
4	34.97053493068565	37.0	35.0	37.0	32.0	37.0
5	35.14980953658106	37.0	35.0	37.0	32.0	37.0
6	35.346685909955745	37.0	35.0	37.0	33.0	37.0
7	35.35862887582104	37.0	35.0	37.0	33.0	37.0
8	35.390490504711465	37.0	35.0	37.0	33.0	37.0
9	37.14192678078982	39.0	37.0	39.0	33.0	39.0
10	36.96291373846034	39.0	37.0	39.0	33.0	39.0
11	37.02985940626398	39.0	37.0	39.0	33.0	39.0
12	36.9535093996917	39.0	37.0	39.0	33.0	39.0
13	37.045672716264605	39.0	37.0	39.0	33.0	39.0
14	38.13955810362432	40.0	37.0	41.0	33.0	41.0
15	38.13791038593239	40.0	37.0	41.0	33.0	41.0
16	38.135635977869335	40.0	37.0	41.0	33.0	41.0
17	38.07994683753716	40.0	37.0	41.0	33.0	41.0
18	38.133129216384766	40.0	37.0	41.0	33.0	41.0
19	38.142532227419565	40.0	37.0	41.0	33.0	41.0
20	38.130139159783475	40.0	37.0	41.0	33.0	41.0
21	38.10605672344499	40.0	37.0	41.0	33.0	41.0
22	38.01539772930959	40.0	37.0	41.0	33.0	41.0
23	38.0310317952422	40.0	37.0	41.0	33.0	41.0
24	37.94866449891989	40.0	37.0	41.0	33.0	41.0
25	37.93640686013519	40.0	37.0	41.0	33.0	41.0
26	37.829264050411396	40.0	37.0	41.0	33.0	41.0
27	37.7049974573897	40.0	37.0	41.0	32.0	41.0
28	37.726302274540835	40.0	37.0	41.0	33.0	41.0
29	37.637606998750606	39.0	37.0	41.0	32.0	41.0
30	37.54866874766817	39.0	37.0	41.0	32.0	41.0
31	37.519479846991956	39.0	37.0	41.0	32.0	41.0
32	37.43535064786773	39.0	36.0	41.0	32.0	41.0
33	37.409098694439315	39.0	36.0	41.0	32.0	41.0
34	37.32431625026721	39.0	36.0	41.0	31.0	41.0
35	37.29732740455917	39.0	36.0	41.0	31.0	41.0
36	37.28205713769795	39.0	36.0	41.0	31.0	41.0
37	37.19481573045941	39.0	36.0	40.0	31.0	41.0
38	37.15451768076764	39.0	36.0	40.0	31.0	41.0
39	37.08703029755843	39.0	36.0	40.0	31.0	41.0
40	36.99292185091408	39.0	36.0	40.0	31.0	41.0
41	36.92630678883588	39.0	36.0	40.0	31.0	41.0
42	36.86281190127502	39.0	35.0	40.0	31.0	41.0
43	36.16537588808797	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	10.0
11	16.0
12	11.0
13	9.0
14	3.0
15	10.0
16	9.0
17	9.0
18	18.0
19	39.0
20	73.0
21	149.0
22	305.0
23	636.0
24	1075.0
25	1855.0
26	3047.0
27	4687.0
28	6946.0
29	9914.0
30	13509.0
31	17848.0
32	23401.0
33	30769.0
34	40428.0
35	54351.0
36	75490.0
37	112322.0
38	189448.0
39	166764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.92738092551015	21.991786638483305	15.265354246026424	24.815478189980123
2	14.397414636672274	21.53770166617319	40.71509089001982	23.349792807134712
3	14.829724774052893	28.58863220843297	33.65553007781848	22.926112939695656
4	11.915747321628917	19.380930821083883	37.58761383657986	31.115708020707338
5	12.653303468173558	38.01740127966987	35.405084955049574	13.924210297107
6	27.720427052311386	39.83520167613119	17.703206344443366	14.74116492711405
7	24.991668470171795	32.753467708849215	22.661097265797707	19.593766555181283
8	25.073058554389956	34.866423337312106	21.991255544948437	18.0692625633495
9	24.113239763504048	14.83755840369216	19.40655608414115	41.64264574866264
10	14.95068132661854	28.263602965095203	33.107043229686006	23.678672478600248
11	31.36864131668709	23.07681073021378	25.1600251207242	20.394522832374932
12	20.88007509662583	26.245845852756972	28.662055889628142	24.212023160989055
13	29.071529005009538	20.214482124055483	26.571937283164466	24.14205158777051
14	21.97080844385611	21.967754656030632	26.43478237778542	29.62665452232784
15	24.25623669776662	28.45107898290277	22.532572630360228	24.760111688970383
16	24.881599335070895	26.539938897688813	23.478981309490774	25.099480457749518
17	21.30787093896009	30.01674272368664	24.74205450878495	23.93333182856832
18	23.8563232660128	25.036014780333076	26.130067462156266	24.977594491497857
19	23.653843855845285	27.081521529868034	26.71679304479907	22.547841569487616
20	22.923059151870177	26.806016758656494	25.517849389839913	24.753074699633412
21	26.23602062236196	25.066950978739	25.60069998127895	23.09632841762009
22	22.929963367823433	29.37279181266207	25.72497586843751	21.97226895107699
23	23.627421952485715	25.217648769257117	25.970208308161713	25.184720970095455
24	23.556122645430005	28.23067516593354	26.57658435159454	21.636617837041918
25	22.534298684348542	27.589379722583292	27.10807620661132	22.76824538645685
26	25.95759483670865	26.003268880707097	27.131178775377972	20.907957507206277
27	22.44932371877004	29.8076246443333	26.090633767192493	21.65241786970417
28	23.09300908302718	24.975868437509543	27.654438680604333	24.276683798858944
29	23.719965000936053	26.022388247962258	28.143443052831856	22.11420369826983
30	21.906280579369938	26.88143204060741	28.0871471381361	23.125140241886548
31	25.650357226788888	26.089040486587894	25.465536676655653	22.795065609967562
32	24.239108931267204	25.489170338957173	28.41868227727597	21.853038452499657
33	22.999403847507114	25.630706765998863	27.726932948113493	23.642956438380537
34	22.011304325889615	29.155176236750872	26.39189657484502	22.44162286251449
35	22.240471186184134	25.06867703272731	31.61028887505095	21.080562906037603
36	25.828804654503738	25.430218956587087	24.7934378082832	23.947538580625974
37	22.657645157821083	26.36228811027626	27.685374879010254	23.294691852892402
38	22.89039689947594	24.78042601667899	27.87444417742242	24.454732906422645
39	24.30602671666027	25.176356246921316	26.565696934129797	23.951920102288614
40	21.68215910765664	24.422867294330707	31.496369311822274	22.398604286190373
41	22.81219337646698	25.487178738201425	26.24226097139663	25.458366913934967
42	23.06167456447011	24.973876836753796	29.169250215424814	22.79519838335128
43	23.542978080442083	24.09026996812111	29.307334534489875	23.05941741694693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	14.5
2	21.0
3	76.5
4	132.0
5	132.0
6	169.0
7	206.0
8	229.0
9	252.0
10	337.0
11	422.0
12	422.0
13	738.0
14	1054.0
15	2481.0
16	3908.0
17	4333.0
18	4758.0
19	4758.0
20	4629.5
21	4501.0
22	5287.0
23	6073.0
24	7861.0
25	9649.0
26	9649.0
27	12325.5
28	15002.0
29	18110.0
30	21218.0
31	24787.0
32	28356.0
33	28356.0
34	32557.0
35	36758.0
36	39324.5
37	41891.0
38	45401.0
39	48911.0
40	48911.0
41	50953.5
42	52996.0
43	54368.0
44	55740.0
45	56508.5
46	57277.0
47	57277.0
48	57505.0
49	57733.0
50	67828.5
51	77924.0
52	63352.5
53	48781.0
54	48781.0
55	54783.0
56	60785.0
57	51259.5
58	41734.0
59	32384.0
60	23034.0
61	23034.0
62	21049.0
63	19064.0
64	15423.0
65	11782.0
66	9704.0
67	7626.0
68	7626.0
69	6161.5
70	4697.0
71	4081.0
72	3465.0
73	3410.0
74	3355.0
75	3355.0
76	2938.5
77	2522.0
78	1790.0
79	1058.0
80	655.0
81	252.0
82	252.0
83	182.5
84	113.0
85	85.5
86	58.0
87	47.5
88	37.0
89	37.0
90	22.5
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	753163.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.106610371051644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.52386910780454	37.93206527630911
2	11.02011514252969	10.382405405265446
3	3.087691986681288	4.363521100872415
4	1.3306493201163871	2.507295162529097
5	0.736636013677726	1.7350212840800654
6	0.4962834195289397	1.4026937806417754
7	0.39232266506546015	1.2936693646079858
8	0.25904638255506535	0.9762237608841482
9	0.20623771854692952	0.8743643866264337
>10	1.6502636458572868	15.862811541925629
>50	0.19245143618799296	6.242824421210004
>100	0.09848981079716293	7.865054893527897
>500	0.0028301669769282166	0.9717663680866357
>1k	0.001981116883849752	2.063387032360662
>5k	5.660333953856432E-4	2.116848181160598
>10k+	5.660333953856432E-4	3.410048039912192
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	14071	1.8682542822735584	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	11507	1.5278233264246917	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	9843	1.306888415920591	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	6035	0.8012873707285142	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3647	0.4842245304137352	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3068	0.4073487412419357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2534	0.3364477543373745	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2429	0.3225065490471518	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1378	0.1829617227612084	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1211	0.16078856768056848	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1210	0.16065579429685206	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	962	0.12772799513518324	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	947	0.12573639437943712	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	935	0.12414311377484025	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	766	0.1017044119267675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.3277338371640667E-4	0.0
4	0.0	0.0	0.0	1.3277338371640667E-4	0.0
5	0.0	0.0	0.0	1.3277338371640667E-4	0.0
6	1.3277338371640667E-4	0.0	0.0	1.3277338371640667E-4	0.0
7	1.3277338371640667E-4	0.0	0.0	1.3277338371640667E-4	0.0
8	1.3277338371640667E-4	0.0	0.0	1.3277338371640667E-4	0.0
9	1.3277338371640667E-4	0.0	0.0	2.6554676743281334E-4	0.0
10	1.3277338371640667E-4	0.0	0.0	2.6554676743281334E-4	0.0
11	1.3277338371640667E-4	0.0	0.0	2.6554676743281334E-4	0.0
12	1.3277338371640667E-4	1.3277338371640667E-4	0.0	2.6554676743281334E-4	0.0
13	1.3277338371640667E-4	1.3277338371640667E-4	0.0	2.6554676743281334E-4	0.0
14	1.3277338371640667E-4	1.3277338371640667E-4	0.0	2.6554676743281334E-4	0.0
15	1.3277338371640667E-4	1.3277338371640667E-4	0.0	2.6554676743281334E-4	0.0
16	1.3277338371640667E-4	1.3277338371640667E-4	0.0	3.9832015114922E-4	0.0
17	1.3277338371640667E-4	1.3277338371640667E-4	0.0	5.310935348656267E-4	0.0
18	1.3277338371640667E-4	1.3277338371640667E-4	0.0	6.638669185820334E-4	0.0
19	1.3277338371640667E-4	1.3277338371640667E-4	0.0	6.638669185820334E-4	0.0
20	1.3277338371640667E-4	1.3277338371640667E-4	0.0	7.9664030229844E-4	0.0
21	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0013277338371640668	1.3277338371640667E-4
22	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0021243741394625067	1.3277338371640667E-4
23	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0021243741394625067	1.3277338371640667E-4
24	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0022571475231789135	1.3277338371640667E-4
25	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0025226942906117268	1.3277338371640667E-4
26	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.0030537878254773537	1.3277338371640667E-4
27	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.00358488136034298	1.3277338371640667E-4
28	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.008630269941566434	1.3277338371640667E-4
29	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.018189953569147713	1.3277338371640667E-4
30	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.03266225239423604	1.3277338371640667E-4
31	1.3277338371640667E-4	1.3277338371640667E-4	0.0	0.06864383938138226	1.3277338371640667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1775	0.0	32.93521	1
TTATACA	1895	0.0	30.459103	2
CGTCTTC	525	0.0	30.304764	37
GATTACG	55	1.853914E-8	30.272728	1
CGACGAG	25	0.005495155	29.6	24
TATACAC	1990	0.0	29.562815	3
GGTATCA	475	0.0	24.926315	1
CCGTCTT	1500	0.0	24.666666	37
GCCGTCT	1520	0.0	24.342106	36
GTATCAA	1290	0.0	23.949612	1
CTGTCCG	45	0.0038246019	20.555555	9
AGGACCG	65	6.898092E-5	19.923077	5
CGATTAG	85	1.2441415E-6	19.588236	16
TTACGGG	85	1.2441415E-6	19.588236	3
TGCCGTC	1905	0.0	19.519686	35
CGTGAAA	50	0.0070326854	18.5	10
TGAGTAC	50	0.0070326854	18.5	34
CGGTGTA	90	2.1502128E-6	18.5	34
CGGCTTA	70	1.2184889E-4	18.5	28
TCGGTGT	90	2.1502128E-6	18.5	33
>>END_MODULE