FastQCFastQC Report
Fri 10 Feb 2017
ERR1630869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791229
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT111541.40970566043459No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG89441.130393350092072No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT59570.7528793813169133Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC48710.6156245537006354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27250.3444009256485796No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.32645416181661696No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC22680.286642678668249No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC19190.2425340830530731Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15090.1907159621298006No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG12590.1591195469326832No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC10730.13561181402602784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9170.1158956509430266No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8700.10995552488596853No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA8400.10616395506231446No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8300.10490009845442974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA13900.031.2769782
CGTCTTC3450.030.02898437
CTTATAC14700.029.9523811
TATACAC14850.029.6498323
GGTATCA5150.027.6601941
CGGATCG509.086418E-625.89999837
GATTACG659.384166E-825.6153851
CCGTTTA451.3227027E-424.66666827
CCGTCTT9500.023.75789537
TTACCGG551.9016878E-523.54545431
GCCGTCT9700.023.26804236
GTATCAA16350.023.195721
TTTAGGC502.701072E-422.1999993
CGGCGTA502.701072E-422.19999912
CGCCGTT502.701072E-422.19999925
AGGACCG603.724397E-521.5833345
CGAACGC555.141148E-420.18181830
GCCGCCG555.141148E-420.18181823
GCCGTTT555.141148E-420.18181826
ACTCGTC609.233348E-418.518
Produced by FastQC (version 0.11.5)