##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630869.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	791229
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4857342185385	31.0	31.0	34.0	28.0	34.0
2	31.47600125880118	31.0	31.0	34.0	28.0	34.0
3	30.946150861507856	31.0	31.0	34.0	27.0	34.0
4	35.095074877184736	37.0	35.0	37.0	32.0	37.0
5	35.29507133838623	37.0	35.0	37.0	33.0	37.0
6	35.488617075461086	37.0	35.0	37.0	33.0	37.0
7	35.49672850717049	37.0	35.0	37.0	33.0	37.0
8	35.521577191938114	37.0	35.0	37.0	33.0	37.0
9	37.29069081138331	39.0	37.0	39.0	34.0	39.0
10	37.117940570934586	39.0	37.0	39.0	33.0	39.0
11	37.18354483973666	39.0	37.0	39.0	33.0	39.0
12	37.112059846138095	39.0	37.0	39.0	33.0	39.0
13	37.187384436111415	39.0	37.0	39.0	33.0	39.0
14	38.327033008143026	40.0	38.0	41.0	34.0	41.0
15	38.332334886613104	40.0	38.0	41.0	34.0	41.0
16	38.32661719931903	40.0	38.0	41.0	34.0	41.0
17	38.306620460069084	40.0	38.0	41.0	33.0	41.0
18	38.33488408539121	40.0	38.0	41.0	34.0	41.0
19	38.35646317311423	40.0	38.0	41.0	34.0	41.0
20	38.351224487474546	40.0	38.0	41.0	34.0	41.0
21	38.3176198041275	40.0	38.0	41.0	34.0	41.0
22	38.24824671492071	40.0	38.0	41.0	34.0	41.0
23	38.24480144180762	40.0	38.0	41.0	34.0	41.0
24	38.204609537820275	40.0	38.0	41.0	33.0	41.0
25	38.17208418801636	40.0	38.0	41.0	33.0	41.0
26	38.08679408868987	40.0	37.0	41.0	33.0	41.0
27	38.00754775166229	40.0	37.0	41.0	33.0	41.0
28	37.99818762962429	40.0	37.0	41.0	33.0	41.0
29	37.932947351525286	40.0	37.0	41.0	33.0	41.0
30	37.81785298567166	40.0	37.0	41.0	33.0	41.0
31	37.79431492020641	40.0	37.0	41.0	33.0	41.0
32	37.70575775154854	40.0	37.0	41.0	33.0	41.0
33	37.68038077471882	40.0	37.0	41.0	33.0	41.0
34	37.618862554330036	40.0	37.0	41.0	32.0	41.0
35	37.564912307309264	40.0	37.0	41.0	32.0	41.0
36	37.54950083983272	40.0	37.0	41.0	32.0	41.0
37	37.47959566699401	40.0	37.0	41.0	32.0	41.0
38	37.43028124601095	39.0	37.0	41.0	32.0	41.0
39	37.35625969220036	39.0	36.0	41.0	31.0	41.0
40	37.26890824274641	39.0	36.0	41.0	31.0	41.0
41	37.20565980266143	39.0	36.0	41.0	31.0	41.0
42	37.13595052759694	39.0	36.0	41.0	31.0	41.0
43	36.49559356393661	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	10.0
10	14.0
11	10.0
12	9.0
13	5.0
14	12.0
15	9.0
16	11.0
17	12.0
18	12.0
19	30.0
20	60.0
21	117.0
22	283.0
23	540.0
24	992.0
25	1648.0
26	2727.0
27	4197.0
28	6672.0
29	9243.0
30	12861.0
31	17238.0
32	22441.0
33	29533.0
34	39359.0
35	53215.0
36	73226.0
37	109424.0
38	190193.0
39	217124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.04545965832901	21.74465293865619	14.706488260667896	24.503399142346908
2	14.492517336953018	21.653781648549284	40.844053997009716	23.009647017487982
3	14.737705518882649	28.79735196763516	33.01812749532689	23.446815018155302
4	11.7820504556835	18.994374574238304	38.64734482684533	30.57623014323287
5	12.527473083013893	38.31343391104219	35.763729590295604	13.395363415648315
6	27.803960673837793	40.15879094421464	17.73557339278515	14.30167498916243
7	25.34803451339625	32.166414527273396	23.444666461921894	19.040884497408463
8	24.800279059539022	35.15139106377546	21.826677232507908	18.221652644177603
9	24.701192701480863	15.028392538696131	19.668136531901638	40.602278227921374
10	15.073259448276035	28.235314934108835	33.55779426689365	23.133631350721473
11	32.23504194108153	23.44921634571028	24.86208164766458	19.453660065543605
12	21.586544477009816	26.196714225590824	28.86838070899828	23.348360588401082
13	28.709008390744017	20.769713951333937	26.7658288561213	23.755448801800743
14	21.904530799553605	21.596655329872895	26.861250030016592	29.637563840556908
15	24.15394279026679	28.441070789872462	23.183300915411344	24.221685504449407
16	24.27919098010816	26.69985554118972	23.974348766286372	25.04660471241575
17	21.72910750237921	29.274963379754787	25.059748821137752	23.936180296728253
18	23.756965429730204	25.622796939950383	26.51798657531511	24.102251055004302
19	24.24784733623262	26.603549667668908	26.854804361316383	22.293798634782092
20	23.112651331030587	26.455046516242454	26.15627081413851	24.27603133858845
21	24.47015971355954	25.688391097899597	26.12998259669451	23.711466591846357
22	22.775833545029315	28.800132452172505	26.187235301031684	22.236798701766492
23	23.132367494113588	25.379125385950214	26.826367587638977	24.66213953229722
24	23.449469117031857	27.286537778569798	26.962737715629736	22.301255388768613
25	23.099507222308585	27.54044657109383	26.341046650211254	23.01899955638633
26	23.70855972164822	25.935727836062632	27.559657191533677	22.79605525075547
27	22.979567230220326	28.781048217393447	25.9711158210834	22.268268731302822
28	21.500475842012868	25.540393489116298	27.74266362835538	25.21646704051545
29	24.038805453288493	25.558719409930625	28.181853799595313	22.220621337185566
30	22.662844764284422	27.0036866697252	27.06232961633105	23.271138949659328
31	23.057547182926815	25.021453965918845	27.16975742800125	24.751241423153093
32	24.681223767076283	25.744379945628886	27.036799712851778	22.53759657444305
33	21.79495443165	25.606998732351823	27.73027783359811	24.867769002400063
34	22.342457114185656	26.695179271740543	28.231396978624396	22.73096663544941
35	23.756207115765474	25.730603908602944	28.931573539392513	21.581615436239066
36	23.687074159314182	25.953927371216174	25.271823959940804	25.087174509528847
37	22.721234939568696	26.446199519987264	27.684273453071107	23.148292087372933
38	23.002948577466196	25.265757448222953	27.676184770780647	24.055109203530204
39	23.715763704313165	25.706843404374712	26.911425137349614	23.665967753962505
40	22.143020541461446	25.257668765932493	30.30525928650239	22.29405140610367
41	23.033028364733852	25.500455620307143	26.469580867233127	24.99693514772588
42	22.95782889656471	25.702293520586327	28.21901118386712	23.12086639898184
43	23.69137127178099	24.450443550476535	28.768915193957756	23.089269983784718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	10.0
2	7.0
3	47.5
4	88.0
5	88.0
6	141.5
7	195.0
8	208.0
9	221.0
10	318.0
11	415.0
12	415.0
13	680.5
14	946.0
15	2459.5
16	3973.0
17	4424.5
18	4876.0
19	4876.0
20	4587.5
21	4299.0
22	4977.0
23	5655.0
24	7456.5
25	9258.0
26	9258.0
27	11715.0
28	14172.0
29	17517.0
30	20862.0
31	25232.0
32	29602.0
33	29602.0
34	34506.5
35	39411.0
36	42909.0
37	46407.0
38	50453.5
39	54500.0
40	54500.0
41	57157.5
42	59815.0
43	61685.0
44	63555.0
45	63774.5
46	63994.0
47	63994.0
48	62603.5
49	61213.0
50	59731.5
51	58250.0
52	65074.0
53	71898.0
54	71898.0
55	60109.0
56	48320.0
57	47459.5
58	46599.0
59	38137.5
60	29676.0
61	29676.0
62	24458.5
63	19241.0
64	15333.0
65	11425.0
66	9381.5
67	7338.0
68	7338.0
69	6078.0
70	4818.0
71	3946.0
72	3074.0
73	3212.0
74	3350.0
75	3350.0
76	2844.0
77	2338.0
78	1650.5
79	963.0
80	604.0
81	245.0
82	245.0
83	186.0
84	127.0
85	91.5
86	56.0
87	39.5
88	23.0
89	23.0
90	14.5
91	6.0
92	4.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	791229.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.3269534152692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.73028083287124	37.86314912761295
2	10.393981036108883	9.630429505180157
3	2.775895040801173	3.857962807226182
4	1.212845882134828	2.24749818726245
5	0.6950901517392728	1.6100704539518846
6	0.42557859074180215	1.1829455728698832
7	0.33178617067706245	1.075944973095078
8	0.24611609190317774	0.9121446979477303
9	0.1950956532755839	0.8134368516737539
>10	1.6226789707426705	15.386867154668332
>50	0.23454440658596562	7.623714676871833
>100	0.1284081808471627	10.72560120323223
>500	0.00467438773961463	1.4320333577990594
>1k	0.002199711877465708	2.3192520321515278
>5k	5.49927969366427E-4	1.898125695122201
>10k+	2.749639846832135E-4	1.420823703334879
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	11154	1.40970566043459	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	8944	1.130393350092072	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	5957	0.7528793813169133	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	4871	0.6156245537006354	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2725	0.3444009256485796	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2583	0.32645416181661696	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	2268	0.286642678668249	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1919	0.2425340830530731	Illumina PCR Primer Index 11 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1509	0.1907159621298006	No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	1259	0.1591195469326832	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1073	0.13561181402602784	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	917	0.1158956509430266	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	870	0.10995552488596853	No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA	840	0.10616395506231446	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	830	0.10490009845442974	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5277132157693917E-4	0.0	0.0	0.0	0.0
6	2.5277132157693917E-4	0.0	0.0	0.0	0.0
7	2.5277132157693917E-4	0.0	0.0	0.0	0.0
8	2.5277132157693917E-4	0.0	0.0	0.0	0.0
9	2.5277132157693917E-4	0.0	0.0	1.2638566078846959E-4	0.0
10	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
11	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
12	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
13	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
14	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
15	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	0.0
16	3.7915698236540876E-4	0.0	0.0	1.2638566078846959E-4	1.2638566078846959E-4
17	7.583139647308175E-4	0.0	0.0	2.5277132157693917E-4	1.2638566078846959E-4
18	7.583139647308175E-4	0.0	0.0	2.5277132157693917E-4	2.5277132157693917E-4
19	7.583139647308175E-4	0.0	0.0	3.7915698236540876E-4	2.5277132157693917E-4
20	7.583139647308175E-4	0.0	0.0	3.7915698236540876E-4	2.5277132157693917E-4
21	7.583139647308175E-4	0.0	0.0	5.055426431538783E-4	2.5277132157693917E-4
22	7.583139647308175E-4	0.0	0.0	0.0011374709470962263	2.5277132157693917E-4
23	7.583139647308175E-4	0.0	0.0	0.001516627929461635	2.5277132157693917E-4
24	7.583139647308175E-4	0.0	0.0	0.0025277132157693915	2.5277132157693917E-4
25	7.583139647308175E-4	0.0	0.0	0.002654098876557861	2.5277132157693917E-4
26	7.583139647308175E-4	0.0	0.0	0.002654098876557861	2.5277132157693917E-4
27	8.84699625519287E-4	0.0	0.0	0.00303325585892327	2.5277132157693917E-4
28	0.0011374709470962263	0.0	0.0	0.007835910968885114	2.5277132157693917E-4
29	0.0011374709470962263	0.0	0.0	0.016935678545654923	2.5277132157693917E-4
30	0.0011374709470962263	0.0	0.0	0.03286027180500209	2.5277132157693917E-4
31	0.0011374709470962263	0.0	0.0	0.07077597004154297	2.5277132157693917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACA	1390	0.0	31.276978	2
CGTCTTC	345	0.0	30.028984	37
CTTATAC	1470	0.0	29.952381	1
TATACAC	1485	0.0	29.649832	3
GGTATCA	515	0.0	27.660194	1
CGGATCG	50	9.086418E-6	25.899998	37
GATTACG	65	9.384166E-8	25.615385	1
CCGTTTA	45	1.3227027E-4	24.666668	27
CCGTCTT	950	0.0	23.757895	37
TTACCGG	55	1.9016878E-5	23.545454	31
GCCGTCT	970	0.0	23.268042	36
GTATCAA	1635	0.0	23.19572	1
TTTAGGC	50	2.701072E-4	22.199999	3
CGGCGTA	50	2.701072E-4	22.199999	12
CGCCGTT	50	2.701072E-4	22.199999	25
AGGACCG	60	3.724397E-5	21.583334	5
CGAACGC	55	5.141148E-4	20.181818	30
GCCGCCG	55	5.141148E-4	20.181818	23
GCCGTTT	55	5.141148E-4	20.181818	26
ACTCGTC	60	9.233348E-4	18.5	18
>>END_MODULE