FastQCFastQC Report
Fri 10 Feb 2017
ERR1630868.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630868.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396209
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT74561.8818350920852378RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG68761.7354477056301092No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT66511.6786594953673442No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC38570.9734761199266044No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC23340.5890830344590859No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC23230.5863067219573508RNA PCR Primer, Index 46 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17160.4331047502706905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16640.41998036389885135No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG9880.249363341064943No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6630.1673359262409486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6380.16102612510064132No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT5780.1458826023639039No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5080.12821515917104356No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4150.10474269892910054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9150.032.3497241
CGTCTTC2700.032.20370537
GGTATCA3050.030.327871
TTATACA10100.029.6732672
TATACAC10350.028.7777793
CCGTCTT9850.026.85786837
GCCGTCT10000.026.45536
CCGCCGT358.859098E-426.4285724
GTATCAA7900.023.1835441
TCACCCG400.001928966623.1255
CGGCAAG802.7144779E-823.12518
TGCCGTC12050.022.10788335
ATGCCGT12600.020.9960334
CGCCTGC555.1349215E-420.18181814
GCCTGCG555.1349215E-420.1818187
TATGCCG13750.019.37454633
TAGAGGC609.222257E-418.55
TTCCGAT609.222257E-418.57
ATTCCGA609.222257E-418.56
GTTCAAA701.2166401E-418.511
Produced by FastQC (version 0.11.5)