FastQCFastQC Report
Fri 10 Feb 2017
ERR1630867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456551
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT87491.9163247917538238RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT85051.8628805982245138No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG82091.7980466585332195No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC47981.0509231170230708No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC33690.7379241311485464RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC25260.5532788231763811RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19780.43324842131547187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19680.4310580855150903No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG11760.2575834901248711No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA7790.17062715884972326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7290.15967547984781547No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA7030.15398060676682343No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC6960.15244737170655634No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT6270.1373340546839236No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT6160.13492468530350388No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6050.1325153159230842No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC5880.12879174506243554No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA5600.12265880482136715No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT5570.1220017040812527No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT5360.11740199890045143No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC5150.11280229371965016No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT5020.10995485717915413No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA4730.10360288335804761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC9450.033.2804221
GATTACG451.0775511E-732.888891
CGTCTTC4400.030.69318237
TTATACA10500.029.952382
CCGGTAT250.005492917329.59999829
CGGTATT250.005492917329.59999830
CACCGGT250.005492917329.59999827
TATACAC10900.029.5321123
CCCTCGG405.932409E-527.7511
GGTATCA3050.027.2950821
CCGTCTT13450.024.62081737
GTATCAA8350.023.7065871
TTACGGG551.8994038E-523.5454543
GCCGTCT14000.023.5214336
TCGCCGA400.001929414523.1251
CGGTAAT400.001929414523.1251
AACGTAA450.003822329720.55555514
ACGCCCT450.003822329720.5555558
AAGACGC450.003822329720.5555555
CGTAATT450.003822329720.55555516
Produced by FastQC (version 0.11.5)