##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630867.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	456551
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.500428210648973	31.0	31.0	34.0	28.0	34.0
2	31.534371844547486	31.0	31.0	34.0	30.0	34.0
3	31.005672969722987	31.0	31.0	34.0	27.0	34.0
4	35.14092839573235	37.0	35.0	37.0	32.0	37.0
5	35.324787373152176	37.0	35.0	37.0	33.0	37.0
6	35.50986198694122	37.0	35.0	37.0	33.0	37.0
7	35.50386484751977	37.0	35.0	37.0	33.0	37.0
8	35.53811293809454	37.0	35.0	37.0	33.0	37.0
9	37.31643781308112	39.0	37.0	39.0	34.0	39.0
10	37.16036981629654	39.0	37.0	39.0	33.0	39.0
11	37.20850025517412	39.0	37.0	39.0	33.0	39.0
12	37.14360498608042	39.0	37.0	39.0	33.0	39.0
13	37.21130169466281	39.0	37.0	39.0	34.0	39.0
14	38.32151282113061	40.0	38.0	41.0	34.0	41.0
15	38.33430876287644	40.0	38.0	41.0	34.0	41.0
16	38.34564594097921	40.0	38.0	41.0	34.0	41.0
17	38.30677843220144	40.0	38.0	41.0	33.0	41.0
18	38.365450957286264	40.0	38.0	41.0	34.0	41.0
19	38.39157290204161	40.0	38.0	41.0	34.0	41.0
20	38.33034644541355	40.0	38.0	41.0	34.0	41.0
21	38.249807798033515	40.0	38.0	41.0	34.0	41.0
22	38.23255452293391	40.0	38.0	41.0	34.0	41.0
23	38.230913961419425	40.0	38.0	41.0	34.0	41.0
24	38.21391476527266	40.0	38.0	41.0	33.0	41.0
25	38.1762278474913	40.0	38.0	41.0	33.0	41.0
26	38.04888172405712	40.0	37.0	41.0	33.0	41.0
27	37.97457677236497	40.0	37.0	41.0	33.0	41.0
28	37.92322873019663	40.0	37.0	41.0	33.0	41.0
29	37.93591296481664	40.0	37.0	41.0	33.0	41.0
30	37.84267036979439	40.0	37.0	41.0	33.0	41.0
31	37.74273848923779	40.0	37.0	41.0	33.0	41.0
32	37.71045293954016	40.0	37.0	41.0	33.0	41.0
33	37.67962834382139	40.0	37.0	41.0	33.0	41.0
34	37.63928892938576	40.0	37.0	41.0	33.0	41.0
35	37.63255364679959	40.0	37.0	41.0	33.0	41.0
36	37.58978734029714	40.0	37.0	41.0	33.0	41.0
37	37.52228776193678	40.0	37.0	41.0	32.0	41.0
38	37.46252883029497	40.0	37.0	41.0	32.0	41.0
39	37.35851854447805	40.0	36.0	41.0	31.0	41.0
40	37.247389667309896	39.0	36.0	41.0	31.0	41.0
41	37.20907631348962	39.0	36.0	41.0	31.0	41.0
42	37.15203777891188	39.0	36.0	41.0	31.0	41.0
43	36.510543181375134	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	10.0
11	6.0
12	12.0
13	8.0
14	4.0
15	9.0
16	5.0
17	11.0
18	21.0
19	27.0
20	46.0
21	89.0
22	183.0
23	357.0
24	609.0
25	1073.0
26	1739.0
27	2559.0
28	3885.0
29	5506.0
30	7567.0
31	10135.0
32	12982.0
33	17037.0
34	22020.0
35	29900.0
36	41240.0
37	60933.0
38	105523.0
39	133043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.11140924015061	23.922190511027246	16.223379206266113	23.743021042556034
2	12.815435734452448	22.525194337543887	42.14512726946168	22.51424265854198
3	13.92922148894647	29.5493822157875	35.725253038543336	20.796143256722686
4	10.976429796452095	19.39498544521861	37.677937404583496	31.950647353745804
5	11.70822098735957	39.358582064216264	35.50118168616431	13.432015262259858
6	26.508758057697822	40.533916254701005	18.141237233080204	14.816088454520962
7	22.55564000516919	34.747925204413086	23.1297270184492	19.56670777196852
8	24.295204697832222	35.50490525702495	22.823518073555856	17.376371971586966
9	22.66033805642743	15.398936811002494	19.64994053238302	42.29078460018705
10	13.573729988544544	29.074736447844817	34.128279206485146	23.22325435712549
11	29.692849210712495	23.773028643021263	25.652993860488753	20.881128285777493
12	18.98035487820638	27.961169726930834	29.50185192891922	23.556623465943563
13	28.421359278591	21.186680129930718	27.31721100161866	23.07474958985962
14	21.06993523177038	23.73382163219443	26.953177191595245	28.243065944439945
15	23.317657830121934	29.83018326539642	23.569984514325892	23.282174390155756
16	24.174736228811238	28.351925633718906	23.65102693894001	23.822311198529846
17	19.796254963848508	31.94407634634466	25.288960050465338	22.970708639341495
18	22.76700740990601	26.118878285229908	26.5711826280087	24.54293167685538
19	25.145493055540346	27.75045942293413	26.9512058893749	20.152841632150626
20	23.294440270637892	26.624188754377933	26.858116617858684	23.22325435712549
21	23.215588181824156	28.29300560068864	25.862828030165304	22.6285781873219
22	19.724411949595993	31.244264058122752	26.932149967911577	22.099174024369674
23	21.627594726547528	26.51270066213851	28.644992563809957	23.214712047504
24	23.20332230134202	27.998186401957287	26.000600152009305	22.79789114469139
25	21.263560916524114	27.760754001195924	27.68737775188314	23.288307330396822
26	23.46353419442735	27.490028496268764	28.325203536954252	20.72123377234964
27	21.43243580673353	29.412924295423732	27.340428561102705	21.814211336740037
28	19.115279563509883	27.89808805587985	30.407993849537075	22.5786385310732
29	22.03236878245804	26.559573848266677	26.784959402125942	24.62309796714934
30	21.136302406521946	26.65069181756255	30.827443155310142	21.385562620605363
31	20.964361046191993	27.548729495718987	27.607211461589177	23.879697996499843
32	20.719262470129294	26.789340073726702	28.468889565459282	24.02250789068472
33	19.225453454269072	26.024693845813502	34.084472490477516	20.66538020943991
34	19.56539357048829	29.29552229652328	27.60545919294887	23.53362494003956
35	20.264329724390045	26.19444487034307	31.87924240665336	21.661982998613517
36	22.045948864420403	26.462541972309772	27.99271056245633	23.49879860081349
37	21.337813300157045	27.230473703923547	28.60929009026374	22.822422905655664
38	21.43594034401414	25.787918545792255	29.185567439344123	23.590573670849476
39	23.705128233209432	26.294981283580587	27.80981752312447	22.190072960085512
40	20.07398954333689	25.36343146767831	33.24031707301046	21.322261915974337
41	21.37921064678426	27.114823973663405	27.485647824668003	24.02031755488434
42	21.824724948581867	25.333204833633044	31.431537769055375	21.410532448729715
43	21.705570681041113	25.37022150865949	30.4185074613789	22.50570034892049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	11.5
2	18.0
3	84.0
4	150.0
5	150.0
6	181.0
7	212.0
8	225.5
9	239.0
10	377.5
11	516.0
12	516.0
13	750.0
14	984.0
15	2445.0
16	3906.0
17	4291.0
18	4676.0
19	4676.0
20	4820.0
21	4964.0
22	6008.0
23	7052.0
24	8469.0
25	9886.0
26	9886.0
27	12064.0
28	14242.0
29	17261.5
30	20281.0
31	21412.5
32	22544.0
33	22544.0
34	24241.5
35	25939.0
36	26680.5
37	27422.0
38	28821.0
39	30220.0
40	30220.0
41	31191.0
42	32162.0
43	30294.0
44	28426.0
45	29079.0
46	29732.0
47	29732.0
48	29073.0
49	28414.0
50	28963.0
51	29512.0
52	37343.5
53	45175.0
54	45175.0
55	33147.0
56	21119.0
57	25740.0
58	30361.0
59	24380.5
60	18400.0
61	18400.0
62	13147.5
63	7895.0
64	6401.5
65	4908.0
66	3954.0
67	3000.0
68	3000.0
69	2413.0
70	1826.0
71	1470.5
72	1115.0
73	877.0
74	639.0
75	639.0
76	479.5
77	320.0
78	241.0
79	162.0
80	115.0
81	68.0
82	68.0
83	50.5
84	33.0
85	26.0
86	19.0
87	12.0
88	5.0
89	5.0
90	4.0
91	3.0
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	456551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.918901875878134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.3220151112349	46.00469360634866
2	9.029676836125127	9.737405185958409
3	2.0873651613177264	3.376453119366511
4	0.8645270536901322	1.8645739750784107
5	0.4993976782969868	1.3463487206568292
6	0.29502835819867207	0.9544563057789375
7	0.23869574212794673	0.900914860859067
8	0.1995305603793433	0.8606774965066231
9	0.1501472079913962	0.7286195317162641
>10	1.0994860878397326	11.817827863099527
>50	0.13515442458041743	5.04672887307795
>100	0.07001976213108757	6.541958391252578
>500	0.005292191323861269	1.7588482280602236
>1k	0.0024425498417821245	3.4713820949422733
>5k	0.0012212749208910622	5.589111747297825
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	8749	1.9163247917538238	RNA PCR Primer, Index 27 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	8505	1.8628805982245138	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	8209	1.7980466585332195	No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	4798	1.0509231170230708	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	3369	0.7379241311485464	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	2526	0.5532788231763811	RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1978	0.43324842131547187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1968	0.4310580855150903	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1176	0.2575834901248711	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	779	0.17062715884972326	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.15967547984781547	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	703	0.15398060676682343	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	696	0.15244737170655634	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	627	0.1373340546839236	No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT	616	0.13492468530350388	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	605	0.1325153159230842	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	588	0.12879174506243554	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	560	0.12265880482136715	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	557	0.1220017040812527	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	536	0.11740199890045143	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	515	0.11280229371965016	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	502	0.10995485717915413	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	473	0.10360288335804761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.1903358003815565E-4	0.0
16	0.0	0.0	0.0	2.1903358003815565E-4	0.0
17	2.1903358003815565E-4	0.0	0.0	4.380671600763113E-4	0.0
18	2.1903358003815565E-4	0.0	0.0	4.380671600763113E-4	0.0
19	2.1903358003815565E-4	0.0	0.0	4.380671600763113E-4	0.0
20	2.1903358003815565E-4	0.0	0.0	0.0010951679001907782	0.0
21	2.1903358003815565E-4	0.0	0.0	0.001314201480228934	0.0
22	2.1903358003815565E-4	0.0	0.0	0.002409369380419712	0.0
23	2.1903358003815565E-4	0.0	0.0	0.003066470120534179	0.0
24	2.1903358003815565E-4	0.0	0.0	0.003723570860648646	0.0
25	2.1903358003815565E-4	0.0	0.0	0.004161638020724957	0.0
26	2.1903358003815565E-4	0.0	0.0	0.004599705180801269	0.0
27	2.1903358003815565E-4	0.0	0.0	0.004818738760839424	0.0
28	2.1903358003815565E-4	0.0	0.0	0.007666175301335448	0.0
29	2.1903358003815565E-4	0.0	0.0	0.01774171998309061	0.0
30	2.1903358003815565E-4	0.0	0.0	0.030007600465227324	0.0
31	2.1903358003815565E-4	0.0	0.0	0.0637387717911033	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	945	0.0	33.280422	1
GATTACG	45	1.0775511E-7	32.88889	1
CGTCTTC	440	0.0	30.693182	37
TTATACA	1050	0.0	29.95238	2
CCGGTAT	25	0.0054929173	29.599998	29
CGGTATT	25	0.0054929173	29.599998	30
CACCGGT	25	0.0054929173	29.599998	27
TATACAC	1090	0.0	29.532112	3
CCCTCGG	40	5.932409E-5	27.75	11
GGTATCA	305	0.0	27.295082	1
CCGTCTT	1345	0.0	24.620817	37
GTATCAA	835	0.0	23.706587	1
TTACGGG	55	1.8994038E-5	23.545454	3
GCCGTCT	1400	0.0	23.52143	36
TCGCCGA	40	0.0019294145	23.125	1
CGGTAAT	40	0.0019294145	23.125	1
AACGTAA	45	0.0038223297	20.555555	14
ACGCCCT	45	0.0038223297	20.555555	8
AAGACGC	45	0.0038223297	20.555555	5
CGTAATT	45	0.0038223297	20.555555	16
>>END_MODULE