FastQCFastQC Report
Fri 10 Feb 2017
ERR1630866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277118
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG120764.357710433822415No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT118394.2721872992732335No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT106163.8308590564308345Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC68722.4798100448184526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52511.8948606730706776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47781.7241752610801175No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC40671.4676058574325739Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC31561.1388650322245397No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29241.0551461832143707No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18530.6686682207579443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17240.6221176538514279No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG15770.5690716590044674No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15610.5632979452796282No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA14230.5134996644028897No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12170.4391631001955845No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT12110.43699795754876986No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11990.4326676722551404No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11190.4037991036309442No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11130.4016339609841295No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA11130.4016339609841295No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG11020.39766453279830255No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT9000.32477139702220714No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8650.3121413982491213No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8250.29770711393702326No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7770.2803859727625055No Hit
ACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT7690.2774991159000859No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7690.2774991159000859No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7410.2673951168816172No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6950.2507956899227044No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6930.2500739757070995No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6570.23708311982621125No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6520.23527883428719898No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6430.2320311203169769No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6310.22770083502334748No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6270.22625740659213764No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6130.2212054070829033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6090.2197619786516935No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5850.21110140806443461No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5730.20677112277080523No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5450.19666712375233658No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5170.18656312473386788No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5160.18620226762606543No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5080.18331541076364583No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4880.17609826860759675No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4850.17501569728418942No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4740.17104626909836243No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4330.1562511276784619No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4310.15552941346285698No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4220.1522816994926349No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4190.15119912816922754No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4180.1508382710614251No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4170.15047741395362266No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4070.14686884287559812No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4060.14650798576779567No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4030.1454254144443883No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4010.1447037002287834No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3990.1439819860131785No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.14362112890537604No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3890.14037341493515398No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3850.13892998650394417No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3830.13820827228833926No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG3800.1371257009649319No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3740.1349605583181172No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3660.13207370145569755No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3660.13207370145569755No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3630.13099113013229022No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3590.1295477017010804No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3580.12918684459327795No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3530.1273825590542657No Hit
ATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT3490.1259391306230559Illumina PCR Primer Index 10 (95% over 21bp)
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3470.125217416407451No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3410.12305227376063627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3390.12233055954503137No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3310.11944370268261173No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3250.11727856003579704No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3220.11619598871238966No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3210.11583513160458722No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3180.11475256028117986No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3160.11403084606557495No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3150.1136699889577725No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3130.11294827474216759No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3120.11258741763436515No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2990.10789627523293327No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2990.10789627523293327No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG2860.10320513283150139No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2830.10212256150809404No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2780.10031827596908177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGTA200.001839526837.032
GTATCTT200.001839526837.036
TCAGAGT355.610764E-737.03
GATTACG308.289222E-637.01
GTTATCG200.001839526837.011
TTACGGG308.289222E-637.03
CTTATAC13650.034.83151
TAGGACC554.965841E-1033.6363644
GACCGTT554.965841E-1033.6363647
CGTCTTC4600.033.38043637
ACTCAGA401.5942715E-632.3751
TTATACA14900.031.7852342
ATTACGG352.3818191E-531.7142872
TTGTTAC303.5924942E-430.83333215
CGTTATT303.5924942E-430.83333210
AGTAGGT303.5924942E-430.8333325
AGCTAGT303.5924942E-430.8333321
TATTGTT303.5924942E-430.83333213
TAGTAGG303.5924942E-430.8333324
TGTATCC303.5924942E-430.8333323
Produced by FastQC (version 0.11.5)