FastQCFastQC Report
Fri 10 Feb 2017
ERR1630865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205711
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT72383.5185284209400565RNA PCR Primer, Index 26 (100% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG71183.4601941558788782No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT60522.9419914345854137No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC40241.9561423550515045No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC26531.289673376727545RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC22941.1151567004195206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.9348065976053784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17240.8380689413789247No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG10030.48757723213634657No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8160.3966730024160108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6770.3291024787201462No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT6000.2916713253058903RNA PCR Primer, Index 26 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA5880.2858378987997725No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT5610.27271268916100744No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4350.21146171084677046No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4130.2007670955855545No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3880.18861412369780906No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3600.17500279518353418No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3440.16722489317537712No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3400.16528041767333784No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3100.15069685140804331No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2850.1385438795202979No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2770.13465492851621935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.13416880964070954No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2520.12250195662847393No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT2420.11764076787337575RNA PCR Primer, Index 26 (95% over 23bp)
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2350.11423793574480703No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2320.11277957911827757No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAAT2250.10937674698970887No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2170.10548779598563032No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2160.1050016771101205No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2120.10305720160808124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACTA200.001838425537.05
CCACGCT200.001838425537.020
GATTACG200.001838425537.01
CGTCTTC3000.035.1537
CTTATAC8100.032.43211
TTATACA8050.032.4037252
TCAGAGT401.5916266E-632.3753
TACTTAT352.3788565E-531.7142851
ATTACTT303.5893024E-430.8333342
TATACAC8700.029.7701173
ACAACTA250.005485988729.59999836
CTTGTAC250.005485988729.5999983
TGCCACA250.005485988729.59999832
TTGTACT250.005485988729.5999984
CGTTATT250.005485988729.59999810
CGCTCAG502.7024544E-729.59999822
TGTTACG250.005485988729.59999816
GACGCTC502.7024544E-729.59999820
TTGTTAC250.005485988729.59999815
CACAACT250.005485988729.59999835
Produced by FastQC (version 0.11.5)