FastQCFastQC Report
Fri 10 Feb 2017
ERR1630864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300351
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG105623.5165523004751105No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT99503.3127907015458575No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT90233.004151809050078TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC59231.9720260628398107No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC33271.1077039863359868RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC29060.9675346511248507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28920.9628734380774493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.953551011982647No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15190.505741615643031No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG15070.50174629017383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10390.3459285968749896No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA9800.3262849133180845No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT8240.27434568221847105No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8160.27168213190567037No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8120.27035035674927005No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8100.26968446917106986No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7800.25969615549806724No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6330.2107534185003546No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6170.2054263178747532No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5960.1984344983036514No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5850.19477211662355046No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5700.18977795978704914No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5500.18311908400504742No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4530.15082353646233906No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4240.14116816657843656No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4040.13450929079643484No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA3940.13117985290543396No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3890.12951513395993355No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3800.12651863985803277No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3790.12618569606893268No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3740.12452097712343224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.11852798891963069No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3450.11486560723952975No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3430.11419971966132958No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3410.11353383208312941No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3340.1112032255594288No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG3340.1112032255594288No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3300.10987145040302845No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3300.10987145040302845No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3210.10687495630112769No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT3130.10421140598832698No Hit
ATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCT3130.10421140598832698TruSeq Adapter, Index 12 (95% over 21bp)
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3130.10421140598832698No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC3010.10021608051912596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACA251.2314554E-437.02
GTCGACC200.001839772137.012
GGCCGAA200.001839772137.029
TGAGTCG200.001839772137.09
GCCGAAA200.001839772137.030
ACAGACA452.6066118E-937.017
CCGAAAG200.001839772137.031
TCCCCGA251.2314554E-437.01
GATTACG452.6066118E-937.01
CTTATAC12700.034.523621
CGTCTTC4550.033.3406637
TTATACA13050.033.3141752
GATACAC303.5932063E-430.8333323
GGTCGTT303.5932063E-430.83333233
CGAAAGG303.5932063E-430.83333232
GTCGTTA303.5932063E-430.83333234
TATACAC14350.030.8118463
GGTATCA3700.030.51
TGTATAA551.8460923E-830.2727287
CAGACAC551.8460923E-830.27272818
Produced by FastQC (version 0.11.5)