FastQCFastQC Report
Fri 10 Feb 2017
ERR1630863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436081
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT94052.1567094186630467RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG85411.9585810892930444No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT78401.7978311368759474No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC52431.20229957278579No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC34620.79388920865619RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC28290.6487326895691397No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19810.45427340333561883No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.4506043601991373No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA13580.3114100362088694No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG13520.3100341450326889No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT7600.17427954898287246No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA7360.16877598427815016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7240.166024201925789No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT6740.15455844212428424No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5030.11534554360313795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC10550.034.0189551
ACCGATG250.00549265129.59999816
TATACAC12350.029.0607283
TTATACA12500.028.7122
CGTCTTC4000.028.67499737
CCGTCTT14900.027.06711437
GCCGTCT15250.026.44590236
GGTATCA3000.024.0500011
TGCCGTC17550.022.98005735
GTATCAA8150.022.6993871
ATGCCGT18150.022.22038534
GTAACAC502.698378E-422.1999993
GCTTTAT2500.021.461
ATTGAGC2700.021.2407426
TATGCCG19250.020.95064733
GTCTGCG555.1360624E-420.18181821
CTTCCCG555.1360624E-420.18181820
CGTCTGC555.1360624E-420.18181820
GCCGCAC656.8895824E-519.92307721
CCAGGAC759.244162E-619.7333343
Produced by FastQC (version 0.11.5)