##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630863.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	436081
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.396717582284026	31.0	31.0	34.0	28.0	34.0
2	31.415026107535066	31.0	31.0	34.0	28.0	34.0
3	30.90238281420195	31.0	31.0	34.0	27.0	34.0
4	35.05098364753337	37.0	35.0	37.0	32.0	37.0
5	35.22081906801718	37.0	35.0	37.0	32.0	37.0
6	35.40374609304235	37.0	35.0	37.0	33.0	37.0
7	35.40613326423302	37.0	35.0	37.0	33.0	37.0
8	35.43718483492746	37.0	35.0	37.0	33.0	37.0
9	37.19379885846895	39.0	37.0	39.0	34.0	39.0
10	37.030365918258305	39.0	37.0	39.0	33.0	39.0
11	37.08066620650751	39.0	37.0	39.0	33.0	39.0
12	37.00862913082661	39.0	37.0	39.0	33.0	39.0
13	37.09742914733731	39.0	37.0	39.0	33.0	39.0
14	38.18901534347977	40.0	38.0	41.0	33.0	41.0
15	38.21195832884258	40.0	38.0	41.0	33.0	41.0
16	38.21270818953359	40.0	38.0	41.0	33.0	41.0
17	38.180746237510924	40.0	38.0	41.0	33.0	41.0
18	38.24490862936014	40.0	38.0	41.0	33.0	41.0
19	38.22376806143813	40.0	38.0	41.0	34.0	41.0
20	38.24111346286584	40.0	38.0	41.0	34.0	41.0
21	38.19301230734657	40.0	38.0	41.0	33.0	41.0
22	38.11460026921604	40.0	37.0	41.0	33.0	41.0
23	38.13118434419294	40.0	37.0	41.0	33.0	41.0
24	38.07528647200864	40.0	37.0	41.0	33.0	41.0
25	38.040455786883626	40.0	37.0	41.0	33.0	41.0
26	37.89691593992859	40.0	37.0	41.0	33.0	41.0
27	37.83347818409882	40.0	37.0	41.0	33.0	41.0
28	37.806407525207476	40.0	37.0	41.0	33.0	41.0
29	37.74538904469583	40.0	37.0	41.0	33.0	41.0
30	37.666931143526085	40.0	37.0	41.0	33.0	41.0
31	37.62775493543631	40.0	37.0	41.0	32.0	41.0
32	37.52666821072232	39.0	37.0	41.0	32.0	41.0
33	37.48877387457835	39.0	37.0	41.0	32.0	41.0
34	37.4377076735744	39.0	36.0	41.0	32.0	41.0
35	37.4012305053419	39.0	36.0	41.0	32.0	41.0
36	37.38382089565929	39.0	36.0	41.0	32.0	41.0
37	37.34782987564237	39.0	36.0	41.0	31.0	41.0
38	37.29782999029997	39.0	36.0	41.0	31.0	41.0
39	37.186584602401844	39.0	36.0	41.0	31.0	41.0
40	37.05805114187502	39.0	36.0	41.0	31.0	41.0
41	37.03195736571875	39.0	36.0	41.0	31.0	41.0
42	36.984576259915016	39.0	36.0	41.0	31.0	41.0
43	36.3499074713184	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	6.0
13	4.0
14	0.0
15	2.0
16	4.0
17	6.0
18	16.0
19	16.0
20	40.0
21	96.0
22	200.0
23	334.0
24	607.0
25	1128.0
26	1796.0
27	2745.0
28	4082.0
29	5640.0
30	7894.0
31	10202.0
32	13162.0
33	17045.0
34	22248.0
35	29627.0
36	41014.0
37	61163.0
38	105627.0
39	111367.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.11186912523132	23.93683742240547	16.460015455844214	23.491277996518996
2	12.978781464911334	22.485730861927028	40.93459701294025	23.60089066022138
3	14.14599581270452	29.431458834482584	35.29229661461976	21.13024873819313
4	10.725071718327559	19.21271506898948	38.215836048807446	31.846377163875516
5	11.800330672512676	38.756331965850386	35.64337817974184	13.799959181895108
6	26.5551583306771	40.83232243551083	17.807930178109114	14.804589055702955
7	22.90102068193753	34.39475693735797	23.106487097580494	19.597735283124006
8	24.958665935915576	34.24455548395826	23.274116505878496	17.522662074247673
9	22.823970776071416	15.091691681132632	19.79861539484637	42.28572214794958
10	13.73254051426226	28.834092748824187	33.75405027964988	23.679316457263674
11	30.382887582811453	23.52957363425602	25.100153411866145	20.987385371066384
12	19.82154691444938	27.65403675005332	28.84532919342966	23.679087142067644
13	29.087486040437444	20.50077852509052	26.707194305645054	23.704541128826982
14	21.372634900396946	23.395194929382384	27.140370710945906	28.091799459274768
15	23.444039066136796	29.959342415743862	22.940004265262644	23.656614252856695
16	24.22806772136369	27.756999273070825	24.049660498852276	23.9652725067132
17	19.93712177324855	31.966767641791314	25.054978318248217	23.04113226671192
18	22.51439526143079	26.181145245952013	26.5787777958682	24.725681696749
19	23.59768024747696	29.807077125579877	26.355654110130917	20.239588516812244
20	24.61194135951807	26.10088492734148	25.80873736759914	23.478436345541308
21	25.85482972200119	25.943574702864836	26.035071466080844	22.166524109053135
22	20.192578901626074	31.46227421052511	26.689307720354705	21.65583916749411
23	23.685049337164426	27.453385953526983	26.383859879242618	22.477704830065974
24	23.785489393025607	29.406692793311333	26.208204439083566	20.59961337457949
25	21.562966513101927	31.463191471309234	25.96214923374327	21.011692781845575
26	23.826078182722934	30.902745132211678	25.845886429356014	19.425290255709374
27	22.08351200809024	30.302168633808858	26.694123339471336	20.920196018629568
28	22.22912715756935	27.612989329963927	28.501585714580546	21.65629779788617
29	21.514810321935602	28.411464842540717	26.714761707114043	23.35896312840963
30	22.431612475663925	28.925360196844164	28.5119048984019	20.13112242909001
31	24.77406720311135	26.691142241922943	26.17518305085523	22.359607504110475
32	21.535907319970374	28.962050628208978	27.965217471066158	21.536824580754494
33	19.276464693485842	30.557167131794323	29.99832599906898	20.168042175650854
34	22.480227297222307	29.45645419084986	27.339416301100023	20.723902210827806
35	20.299898413368158	27.720308841706014	32.26396013584632	19.715832609079506
36	22.12708189533596	28.123903586718978	26.485905141476014	23.263109376469053
37	21.033248410272403	26.67577812378893	29.602986601113095	22.68798686482557
38	21.411618483722062	25.129964387350057	29.360141808517227	24.098275320410657
39	24.400512748778326	26.06878079989727	27.447882388822258	22.082824062502148
40	19.940332185992972	25.081349565791676	33.47038738216065	21.5079308660547
41	21.60814160671985	26.426512505704213	27.124318647223795	24.841027240352137
42	21.50403250772219	25.044659134426862	31.913107885920276	21.538200471930676
43	22.03329198015965	25.09648436872966	30.64705868863812	22.22316496247257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	17.5
2	22.0
3	96.5
4	171.0
5	171.0
6	217.5
7	264.0
8	256.5
9	249.0
10	383.5
11	518.0
12	518.0
13	752.0
14	986.0
15	2159.0
16	3332.0
17	3826.0
18	4320.0
19	4320.0
20	4682.0
21	5044.0
22	5991.0
23	6938.0
24	8599.5
25	10261.0
26	10261.0
27	12045.5
28	13830.0
29	15745.0
30	17660.0
31	19568.5
32	21477.0
33	21477.0
34	23011.5
35	24546.0
36	25862.5
37	27179.0
38	27463.5
39	27748.0
40	27748.0
41	27765.5
42	27783.0
43	27627.0
44	27471.0
45	27428.0
46	27385.0
47	27385.0
48	27667.5
49	27950.0
50	34648.5
51	41347.0
52	32116.0
53	22885.0
54	22885.0
55	29338.5
56	35792.0
57	29460.0
58	23128.0
59	17638.5
60	12149.0
61	12149.0
62	10724.5
63	9300.0
64	7936.5
65	6573.0
66	5409.0
67	4245.0
68	4245.0
69	3474.0
70	2703.0
71	2245.0
72	1787.0
73	1180.0
74	573.0
75	573.0
76	402.0
77	231.0
78	172.0
79	113.0
80	78.5
81	44.0
82	44.0
83	40.5
84	37.0
85	27.5
86	18.0
87	13.0
88	8.0
89	8.0
90	4.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	436081.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.90434165672976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.56126466248621	52.71956837242669
2	8.482477323272738	10.332393939841307
3	1.9308833945701476	3.5279754588661922
4	0.7972873986134718	1.942330564950407
5	0.4688258929833122	1.4276766181888534
6	0.310494881254872	1.1346291798367478
7	0.20891209466610028	0.890655751283707
8	0.16864226466419555	0.8216836883898248
9	0.12776996508301589	0.7003571046195988
>10	0.8314394836665876	9.562878967773045
>50	0.07817633845216926	3.2527772326606454
>100	0.028188583572612627	2.8430325985457254
>500	0.0018792389048408417	0.7775991736924177
>1k	0.00225508668580901	2.9636669125097828
>5k	0.0015033911238726734	7.102774436415081
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	9405	2.1567094186630467	RNA PCR Primer, Index 17 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	8541	1.9585810892930444	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	7840	1.7978311368759474	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	5243	1.20229957278579	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	3462	0.79388920865619	RNA PCR Primer, Index 17 (95% over 22bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	2829	0.6487326895691397	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1981	0.45427340333561883	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1965	0.4506043601991373	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1358	0.3114100362088694	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1352	0.3100341450326889	No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT	760	0.17427954898287246	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	736	0.16877598427815016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	724	0.166024201925789	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	674	0.15455844212428424	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	503	0.11534554360313795	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	4.5863039206019065E-4	0.0	0.0	0.0	0.0
4	4.5863039206019065E-4	0.0	0.0	0.0	0.0
5	4.5863039206019065E-4	0.0	0.0	0.0	0.0
6	4.5863039206019065E-4	0.0	0.0	0.0	0.0
7	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
8	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
9	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
10	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
11	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
12	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
13	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
14	4.5863039206019065E-4	0.0	0.0	2.2931519603009532E-4	0.0
15	4.5863039206019065E-4	0.0	0.0	4.5863039206019065E-4	0.0
16	4.5863039206019065E-4	0.0	0.0	4.5863039206019065E-4	0.0
17	4.5863039206019065E-4	0.0	0.0	4.5863039206019065E-4	0.0
18	4.5863039206019065E-4	0.0	0.0	6.87945588090286E-4	0.0
19	4.5863039206019065E-4	0.0	0.0	6.87945588090286E-4	0.0
20	4.5863039206019065E-4	0.0	0.0	0.0011465759801504766	0.0
21	4.5863039206019065E-4	0.0	0.0	0.001375891176180572	0.0
22	4.5863039206019065E-4	0.0	0.0	0.0032104127444213346	0.0
23	4.5863039206019065E-4	0.0	0.0	0.004356988724571811	0.0
24	4.5863039206019065E-4	0.0	0.0	0.005503564704722288	0.0
25	4.5863039206019065E-4	0.0	0.0	0.005503564704722288	0.0
26	4.5863039206019065E-4	0.0	0.0	0.0061915102928125736	0.0
27	4.5863039206019065E-4	0.0	0.0	0.006420825488842669	0.0
28	4.5863039206019065E-4	0.0	0.0	0.01077781421341448	0.0
29	4.5863039206019065E-4	0.0	0.0	0.019033161270497912	0.0
30	4.5863039206019065E-4	0.0	0.0	0.03049892107200268	0.0
31	4.5863039206019065E-4	0.0	0.0	0.06283236371224613	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1055	0.0	34.018955	1
ACCGATG	25	0.005492651	29.599998	16
TATACAC	1235	0.0	29.060728	3
TTATACA	1250	0.0	28.712	2
CGTCTTC	400	0.0	28.674997	37
CCGTCTT	1490	0.0	27.067114	37
GCCGTCT	1525	0.0	26.445902	36
GGTATCA	300	0.0	24.050001	1
TGCCGTC	1755	0.0	22.980057	35
GTATCAA	815	0.0	22.699387	1
ATGCCGT	1815	0.0	22.220385	34
GTAACAC	50	2.698378E-4	22.199999	3
GCTTTAT	250	0.0	21.46	1
ATTGAGC	270	0.0	21.240742	6
TATGCCG	1925	0.0	20.950647	33
GTCTGCG	55	5.1360624E-4	20.181818	21
CTTCCCG	55	5.1360624E-4	20.181818	20
CGTCTGC	55	5.1360624E-4	20.181818	20
GCCGCAC	65	6.8895824E-5	19.923077	21
CCAGGAC	75	9.244162E-6	19.733334	3
>>END_MODULE