FastQCFastQC Report
Fri 10 Feb 2017
ERR1630862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292765
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT110133.761720150974331No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG109893.7535224497463835No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT96583.2988916024798045TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC61062.0856318207435995No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC38761.3239287483134938RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC29291.000461120694072TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28600.9768927296637234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26590.9082369818796645No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG14190.48468908510238584No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14090.4812733762574078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10790.368554984373132No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA9530.32551705292640853No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT8190.2797465544037026No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8080.2759892746742268No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT7470.2551534507198606No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7220.24661417860741552No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6120.20904138131265693No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5940.20289310539169642No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5880.2008436800847096No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5610.19162126620326883No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5380.1837651358598193No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5090.17385958020938297No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4980.1701023004799071No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4000.13662835379912217No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3910.1335542158386419No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3630.12399023107270338No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT3580.12228237665021433TruSeq Adapter, Index 11 (95% over 22bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3340.11408467542226701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3290.11237682099977798No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3260.11135210834628458No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3230.11032739569279114No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3110.10622854507881749No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3100.10588697419431968No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3020.10315440711833723No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3020.10315440711833723No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2930.10008026915785699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG200.001839696537.026
TTAGCGG200.001839696537.027
GTTGTAT200.001839696537.01
GATTACG200.001839696537.01
CTTATAC12250.035.7918361
TTATACA13050.033.4559362
CGTCTTC4800.032.76041437
GCCTGTT303.5929863E-430.83333211
CGCCGAG303.5929863E-430.8333322
TATACAC14350.030.5540073
CCCTGCC250.00548973729.611
CCCCTGC250.00548973729.610
TTGGCAT250.00548973729.632
TAGCGGG250.00548973729.628
TCTAGAT250.00548973729.62
CGCCTGT250.00548973729.610
TTCTGCG405.9252485E-527.7518
GCCCCTA358.8535476E-426.4285734
GCGACTC358.8535476E-426.4285720
ACGTTGA358.8535476E-426.4285731
Produced by FastQC (version 0.11.5)