##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630862.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292765
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237166327942205	31.0	31.0	34.0	28.0	34.0
2	31.247034310795346	31.0	31.0	34.0	28.0	34.0
3	30.709685242429934	31.0	31.0	34.0	26.0	34.0
4	34.87926835516541	37.0	35.0	37.0	32.0	37.0
5	35.084928184721534	35.0	35.0	37.0	32.0	37.0
6	35.29043430737964	37.0	35.0	37.0	33.0	37.0
7	35.26967021331102	37.0	35.0	37.0	32.0	37.0
8	35.30985261216334	37.0	35.0	37.0	32.0	37.0
9	37.093850016224614	39.0	37.0	39.0	33.0	39.0
10	36.9045036121121	39.0	37.0	39.0	33.0	39.0
11	36.964572267859886	39.0	37.0	39.0	33.0	39.0
12	36.86407869793179	39.0	37.0	39.0	33.0	39.0
13	36.95251823134596	39.0	37.0	39.0	33.0	39.0
14	37.89118917903438	40.0	37.0	41.0	33.0	41.0
15	37.90756067152836	39.0	37.0	41.0	33.0	41.0
16	37.91431352791488	40.0	37.0	41.0	33.0	41.0
17	37.87420627465715	40.0	37.0	41.0	33.0	41.0
18	37.98297269140778	40.0	37.0	41.0	33.0	41.0
19	37.95836934059741	40.0	37.0	41.0	33.0	41.0
20	37.894246238450634	40.0	37.0	41.0	33.0	41.0
21	37.797141051696755	40.0	37.0	41.0	32.0	41.0
22	37.5978207777569	39.0	37.0	41.0	32.0	41.0
23	37.68782129011323	39.0	37.0	41.0	32.0	41.0
24	37.62735641213943	39.0	37.0	41.0	32.0	41.0
25	37.56032995747442	39.0	37.0	41.0	32.0	41.0
26	37.450856488992876	39.0	36.0	41.0	32.0	41.0
27	37.241459873960345	39.0	36.0	41.0	31.0	41.0
28	37.258729697880554	39.0	36.0	41.0	31.0	41.0
29	37.1184157942377	39.0	36.0	40.0	31.0	41.0
30	36.976435024678494	39.0	36.0	40.0	31.0	41.0
31	36.93897494577562	39.0	35.0	40.0	31.0	41.0
32	36.68397861766263	39.0	35.0	40.0	30.0	41.0
33	36.71943025976466	39.0	35.0	40.0	30.0	41.0
34	36.718122043277035	39.0	35.0	40.0	30.0	41.0
35	36.690967157959456	39.0	35.0	40.0	30.0	41.0
36	36.66712209451266	39.0	35.0	40.0	30.0	41.0
37	36.572657250695954	39.0	35.0	40.0	30.0	41.0
38	36.46711867880382	39.0	35.0	40.0	30.0	41.0
39	36.30752309873107	39.0	35.0	40.0	30.0	41.0
40	36.06971803323485	38.0	35.0	40.0	29.0	41.0
41	36.02353764965074	38.0	35.0	40.0	28.0	41.0
42	35.894509248031696	38.0	35.0	40.0	28.0	41.0
43	35.050364626919205	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	6.0
10	9.0
11	8.0
12	17.0
13	9.0
14	7.0
15	7.0
16	13.0
17	10.0
18	18.0
19	38.0
20	64.0
21	107.0
22	214.0
23	344.0
24	638.0
25	1076.0
26	1792.0
27	2632.0
28	3723.0
29	5031.0
30	6533.0
31	8370.0
32	10479.0
33	13395.0
34	17531.0
35	22748.0
36	30813.0
37	44589.0
38	67631.0
39	54908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.64519324372791	23.536966508974775	16.9958840708418	24.82195617645552
2	13.340392464946287	21.27440097006131	41.82842894471675	23.55677762027565
3	13.594862773897153	31.122231141017544	34.43034515737878	20.852560927706524
4	9.916827489624785	19.926903830717468	33.934042662203474	36.222226017454275
5	12.402097245230816	38.59102010144655	33.15628575820197	15.850596895120662
6	25.982272471094564	39.55937355899783	16.448004372107324	18.010349597800285
7	23.143476850033302	33.47394668078493	19.924512834525984	23.458063634655783
8	25.901320171468583	31.455945895171894	23.521254248287875	19.121479685071645
9	22.017659214728535	12.819496866087135	16.977780813963417	48.18506310522091
10	15.327310300070021	26.863866923983398	30.86878554472017	26.94003723122641
11	30.157976534080234	21.844141205403652	23.715949652451627	24.281932608064487
12	19.610950762557	28.228101036667635	23.889126090892013	28.271822109883356
13	32.0465219544686	18.370365310060972	25.22535139104743	24.357761344423
14	22.402951172442062	23.812955783649002	24.318139121821254	29.465953922087678
15	26.721773436032315	27.138489915119635	20.611411883251073	25.52832476559698
16	26.77676634843646	24.030536437074105	20.858367632742986	28.334329581746452
17	19.73869827335918	32.25180605605178	21.474903079261523	26.534592591327517
18	26.06868990487251	20.940686215906958	23.97520195378546	29.015421925435074
19	28.87947671340495	25.200075145594592	24.0623025293324	21.85814561166806
20	24.629651768483253	23.49631957371954	24.202005021092003	27.672023636705205
21	28.010178812358035	25.532082045326455	23.970078390517994	22.487660751797517
22	23.873072259320615	31.306337847761856	24.050689119259474	20.769900773658055
23	24.064010383754887	22.24856113264905	26.07620446433146	27.611224019264597
24	26.141444503270538	27.679196625279662	26.707085887998904	19.472272983450893
25	21.81749867641282	30.034327873892032	24.26963605622257	23.87853739347258
26	27.41038033917989	25.795091626389766	24.855771694020802	21.938756340409544
27	25.18743702286817	29.70607825388964	25.558383003432787	19.548101719809406
28	18.162690212286307	26.069031475757004	29.098423650368044	26.669854661588648
29	28.356190118354313	22.88593240312196	26.73270370433624	22.025173774187486
30	22.819326080644885	24.33658394958414	28.6530152169829	24.191074752788072
31	22.325756152545555	24.744419585674517	27.87115946236743	25.0586647994125
32	24.628968626714258	27.281949686608716	27.645722678598876	20.44335900807815
33	18.277799600362066	22.687138148344236	30.751626731337424	28.283435519956278
34	18.919611292333443	28.263965979539908	30.6768227076324	22.139600020494253
35	19.775246358000445	26.612129182108518	34.49456048366437	19.118063976226665
36	23.30128259867129	23.11751746281147	23.491537581336566	30.089662357180675
37	21.33554215838642	25.169675336874285	29.271258517923933	24.223523986815366
38	20.994312844773113	21.91313852407221	29.652451625023485	27.440097006131197
39	25.978515191365087	22.677915734462793	26.794528034430343	24.549041039741773
40	19.17988830632077	22.058306149983775	37.41362526258262	21.34818028111284
41	22.915649070073265	23.748398886478917	25.242771506156814	28.093180537291
42	22.393387187676122	22.14608986729971	33.298720817037555	22.16180212798661
43	23.971103103171483	21.135381620070707	31.465509879937837	23.428005396819977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	7.0
2	8.0
3	41.0
4	74.0
5	74.0
6	124.0
7	174.0
8	197.0
9	220.0
10	344.0
11	468.0
12	468.0
13	715.5
14	963.0
15	2583.5
16	4204.0
17	4229.0
18	4254.0
19	4254.0
20	3512.0
21	2770.0
22	2222.5
23	1675.0
24	1856.0
25	2037.0
26	2037.0
27	2415.5
28	2794.0
29	3412.0
30	4030.0
31	5117.5
32	6205.0
33	6205.0
34	7823.0
35	9441.0
36	10446.5
37	11452.0
38	12929.0
39	14406.0
40	14406.0
41	15490.0
42	16574.0
43	17930.0
44	19286.0
45	20737.0
46	22188.0
47	22188.0
48	23231.0
49	24274.0
50	24363.5
51	24453.0
52	34020.5
53	43588.0
54	43588.0
55	31504.5
56	19421.0
57	24540.5
58	29660.0
59	23075.5
60	16491.0
61	16491.0
62	10976.0
63	5461.0
64	4116.5
65	2772.0
66	2181.5
67	1591.0
68	1591.0
69	1244.0
70	897.0
71	710.0
72	523.0
73	323.0
74	123.0
75	123.0
76	104.0
77	85.0
78	82.0
79	79.0
80	54.0
81	29.0
82	29.0
83	23.0
84	17.0
85	25.0
86	33.0
87	25.0
88	17.0
89	17.0
90	17.5
91	18.0
92	11.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	292765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.824910764606425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99323307192729	17.152323535941797
2	9.85935685509885	4.6979659453828155
3	4.295278920732319	3.0700391098662747
4	2.541899040873966	2.422420712858436
5	1.720405442215882	2.0494253069868322
6	1.275967369643446	1.8239885232182809
7	0.9619933764390475	1.604358444486192
8	0.8028558730340785	1.530237562550168
9	0.6494530544364955	1.3925844960975526
>10	5.082364410546085	24.45613375915837
>50	0.5232899886739975	8.464468088740116
>100	0.26236182993792206	10.34720680409202
>500	0.015770383220312253	2.545044660393148
>1k	0.01003569841292598	5.544037026283879
>5k	0.002867342403693137	5.3845234232234045
>10k+	0.002867342403693137	7.515242600720715
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	11013	3.761720150974331	No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	10989	3.7535224497463835	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	9658	3.2988916024798045	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	6106	2.0856318207435995	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	3876	1.3239287483134938	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	2929	1.000461120694072	TruSeq Adapter, Index 11 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2860	0.9768927296637234	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2659	0.9082369818796645	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	1419	0.48468908510238584	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1409	0.4812733762574078	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1079	0.368554984373132	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	953	0.32551705292640853	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	819	0.2797465544037026	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	808	0.2759892746742268	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	747	0.2551534507198606	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	722	0.24661417860741552	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	612	0.20904138131265693	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	594	0.20289310539169642	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	588	0.2008436800847096	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	561	0.19162126620326883	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	538	0.1837651358598193	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	509	0.17385958020938297	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	498	0.1701023004799071	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	400	0.13662835379912217	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	391	0.1335542158386419	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	363	0.12399023107270338	No Hit
ATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCT	358	0.12228237665021433	TruSeq Adapter, Index 11 (95% over 22bp)
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	334	0.11408467542226701	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.11237682099977798	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	326	0.11135210834628458	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	323	0.11032739569279114	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	311	0.10622854507881749	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	310	0.10588697419431968	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	302	0.10315440711833723	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	302	0.10315440711833723	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	293	0.10008026915785699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	3.415708844978054E-4	0.0
12	0.0	0.0	0.0	6.831417689956109E-4	0.0
13	0.0	0.0	0.0	6.831417689956109E-4	0.0
14	0.0	0.0	0.0	6.831417689956109E-4	0.0
15	0.0	0.0	0.0	6.831417689956109E-4	0.0
16	0.0	0.0	0.0	6.831417689956109E-4	0.0
17	0.0	0.0	0.0	6.831417689956109E-4	0.0
18	0.0	0.0	0.0	6.831417689956109E-4	0.0
19	0.0	0.0	0.0	6.831417689956109E-4	0.0
20	0.0	0.0	0.0	0.0010247126534934162	0.0
21	0.0	0.0	0.0	0.001707854422489027	0.0
22	0.0	0.0	0.0	0.0030741379604802485	0.0
23	0.0	0.0	0.0	0.004098850613973665	0.0
24	0.0	0.0	0.0	0.004440421498471471	0.0
25	0.0	0.0	0.0	0.004440421498471471	0.0
26	0.0	0.0	0.0	0.004781992382969276	0.0
27	0.0	0.0	0.0	0.006489846805458303	0.0
28	0.0	0.0	0.0	0.01639540245589466	0.0
29	0.0	0.0	0.0	0.035523371987771765	0.0
30	0.0	0.0	0.0	0.05875019213362253	0.0
31	0.0	0.0	0.0	0.09632298942838112	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	20	0.0018396965	37.0	26
TTAGCGG	20	0.0018396965	37.0	27
GTTGTAT	20	0.0018396965	37.0	1
GATTACG	20	0.0018396965	37.0	1
CTTATAC	1225	0.0	35.791836	1
TTATACA	1305	0.0	33.455936	2
CGTCTTC	480	0.0	32.760414	37
GCCTGTT	30	3.5929863E-4	30.833332	11
CGCCGAG	30	3.5929863E-4	30.833332	2
TATACAC	1435	0.0	30.554007	3
CCCTGCC	25	0.005489737	29.6	11
CCCCTGC	25	0.005489737	29.6	10
TTGGCAT	25	0.005489737	29.6	32
TAGCGGG	25	0.005489737	29.6	28
TCTAGAT	25	0.005489737	29.6	2
CGCCTGT	25	0.005489737	29.6	10
TTCTGCG	40	5.9252485E-5	27.75	18
GCCCCTA	35	8.8535476E-4	26.42857	34
GCGACTC	35	8.8535476E-4	26.42857	20
ACGTTGA	35	8.8535476E-4	26.42857	31
>>END_MODULE