FastQCFastQC Report
Fri 10 Feb 2017
ERR1630861.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630861.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269673
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT69022.559396009240821TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG60562.245682734274473No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT51141.8963707898083975No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC35831.3286461751825358No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC26400.9789634112425049RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC19300.7156815847341039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.5643872393602622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14560.5399131540791996No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG9200.3411539160390547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5400.2002425159359669No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA5300.19653432119641195No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT4840.17947662539445922No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4770.17688088907677077No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT4610.17094777749348283No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3120.1156956758741142No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2790.10345863323358288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6650.031.7142851
CGTCTTC2950.031.35593237
TTATACA7100.029.964792
TTGCACG250.00548897829.610
TTGGGCC250.00548897829.64
TATACAC7500.028.3666653
CCGTCTT10150.025.69950737
GCCGTCT10400.024.90384536
CGACCTG451.3195461E-424.66666635
GCCGCTA451.3195461E-424.66666637
CGCCGCT451.3195461E-424.66666636
GGTATCA2450.023.4081631
TTAAGGG400.001927376723.1250023
CTCGCCG400.001927376723.12500234
GTATCAA6300.022.6111131
TGCCGTC12200.021.2295135
GCTGTGG450.003818331520.5555558
GCATATA450.003818331520.5555551
ATGCCGT12750.020.31372534
TCGCCGC555.129075E-420.18181835