Basic Statistics
Measure | Value |
---|---|
Filename | ERR1630861.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 269673 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT | 6902 | 2.559396009240821 | TruSeq Adapter, Index 11 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG | 6056 | 2.245682734274473 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT | 5114 | 1.8963707898083975 | No Hit |
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC | 3583 | 1.3286461751825358 | No Hit |
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC | 2640 | 0.9789634112425049 | RNA PCR Primer, Index 11 (95% over 22bp) |
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC | 1930 | 0.7156815847341039 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1522 | 0.5643872393602622 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT | 1456 | 0.5399131540791996 | No Hit |
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG | 920 | 0.3411539160390547 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 540 | 0.2002425159359669 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA | 530 | 0.19653432119641195 | No Hit |
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT | 484 | 0.17947662539445922 | No Hit |
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA | 477 | 0.17688088907677077 | No Hit |
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT | 461 | 0.17094777749348283 | No Hit |
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA | 312 | 0.1156956758741142 | No Hit |
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT | 279 | 0.10345863323358288 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTTATAC | 665 | 0.0 | 31.714285 | 1 |
CGTCTTC | 295 | 0.0 | 31.355932 | 37 |
TTATACA | 710 | 0.0 | 29.96479 | 2 |
TTGCACG | 25 | 0.005488978 | 29.6 | 10 |
TTGGGCC | 25 | 0.005488978 | 29.6 | 4 |
TATACAC | 750 | 0.0 | 28.366665 | 3 |
CCGTCTT | 1015 | 0.0 | 25.699507 | 37 |
GCCGTCT | 1040 | 0.0 | 24.903845 | 36 |
CGACCTG | 45 | 1.3195461E-4 | 24.666666 | 35 |
GCCGCTA | 45 | 1.3195461E-4 | 24.666666 | 37 |
CGCCGCT | 45 | 1.3195461E-4 | 24.666666 | 36 |
GGTATCA | 245 | 0.0 | 23.408163 | 1 |
TTAAGGG | 40 | 0.0019273767 | 23.125002 | 3 |
CTCGCCG | 40 | 0.0019273767 | 23.125002 | 34 |
GTATCAA | 630 | 0.0 | 22.611113 | 1 |
TGCCGTC | 1220 | 0.0 | 21.22951 | 35 |
GCTGTGG | 45 | 0.0038183315 | 20.555555 | 8 |
GCATATA | 45 | 0.0038183315 | 20.555555 | 1 |
ATGCCGT | 1275 | 0.0 | 20.313725 | 34 |
TCGCCGC | 55 | 5.129075E-4 | 20.181818 | 35 |