##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630861.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	269673
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2936185676727	31.0	31.0	34.0	28.0	34.0
2	31.31261935751818	31.0	31.0	34.0	28.0	34.0
3	30.750850103644044	31.0	31.0	34.0	26.0	34.0
4	34.92803135649472	37.0	35.0	37.0	32.0	37.0
5	35.088759349285986	37.0	35.0	37.0	32.0	37.0
6	35.284778231413604	37.0	35.0	37.0	32.0	37.0
7	35.27753241889251	37.0	35.0	37.0	32.0	37.0
8	35.317117397737256	37.0	35.0	37.0	32.0	37.0
9	37.07857664653117	39.0	37.0	39.0	33.0	39.0
10	36.88865032836065	39.0	37.0	39.0	33.0	39.0
11	36.93626354881653	39.0	37.0	39.0	33.0	39.0
12	36.870765705131774	39.0	37.0	39.0	33.0	39.0
13	36.9480630244778	39.0	37.0	39.0	33.0	39.0
14	37.972025378884794	40.0	37.0	41.0	33.0	41.0
15	37.98270127153997	40.0	37.0	41.0	33.0	41.0
16	37.99322512821083	40.0	37.0	41.0	33.0	41.0
17	37.96124565677691	40.0	37.0	41.0	33.0	41.0
18	38.00526934472491	40.0	37.0	41.0	33.0	41.0
19	37.99656250347643	40.0	37.0	41.0	33.0	41.0
20	37.954029509813736	40.0	37.0	41.0	33.0	41.0
21	37.91559407133825	40.0	37.0	41.0	33.0	41.0
22	37.82309686175479	40.0	37.0	41.0	33.0	41.0
23	37.854712930104235	40.0	37.0	41.0	33.0	41.0
24	37.793909660959756	39.0	37.0	41.0	33.0	41.0
25	37.75506261286818	39.0	37.0	41.0	32.0	41.0
26	37.59512075736169	39.0	37.0	41.0	32.0	41.0
27	37.4732954355831	39.0	37.0	41.0	32.0	41.0
28	37.48523582264446	39.0	37.0	41.0	32.0	41.0
29	37.40314380750019	39.0	36.0	41.0	32.0	41.0
30	37.3205993925977	39.0	36.0	40.0	32.0	41.0
31	37.29252465022453	39.0	36.0	40.0	31.0	41.0
32	37.16059820597538	39.0	36.0	40.0	31.0	41.0
33	37.145505853385394	39.0	36.0	40.0	31.0	41.0
34	37.06780434081276	39.0	36.0	40.0	31.0	41.0
35	37.05784042154758	39.0	36.0	40.0	31.0	41.0
36	37.03550596463124	39.0	36.0	40.0	31.0	41.0
37	36.95088866886933	39.0	36.0	40.0	31.0	41.0
38	36.88673319168029	39.0	36.0	40.0	31.0	41.0
39	36.753267846614236	39.0	35.0	40.0	30.0	41.0
40	36.61054684747824	39.0	35.0	40.0	30.0	41.0
41	36.58814193486185	39.0	35.0	40.0	30.0	41.0
42	36.5358823463973	39.0	35.0	40.0	30.0	41.0
43	35.82961215991219	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	5.0
11	9.0
12	11.0
13	3.0
14	4.0
15	5.0
16	10.0
17	8.0
18	14.0
19	16.0
20	36.0
21	83.0
22	148.0
23	288.0
24	518.0
25	822.0
26	1320.0
27	2021.0
28	2916.0
29	4059.0
30	5390.0
31	7015.0
32	9139.0
33	11529.0
34	14986.0
35	20098.0
36	27694.0
37	40416.0
38	65869.0
39	55238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.32277981110456	23.901910832749294	16.805909379136956	23.969399977009193
2	13.322802060272998	22.115673426705676	41.107563604810274	23.453960908211055
3	14.287303512031238	29.998553804051575	34.45765797836639	21.256484705550797
4	10.753393925235377	19.333414913617602	36.96736417809718	32.94582698304984
5	12.0304961935381	38.85223956421295	34.818465326525086	14.298798915723859
6	26.86920826334116	40.03181631086538	17.72554167454658	15.373433751246882
7	22.970041494699135	34.406484890960535	22.574006296514668	20.049467317825663
8	25.254289454264978	34.02565329120824	22.965220841537715	17.754836412989064
9	22.705647209768866	14.334397585223584	19.233664475123575	43.72629072988397
10	14.057766257652787	28.099216458451533	33.2261665053602	24.616850778535486
11	30.16097273364408	23.098345032687735	24.840825740804604	21.899856492863577
12	19.28520838200339	27.807010712974602	28.39438875972011	24.5133921453019
13	29.58434845164329	20.243405902704385	26.10049949383142	24.071746151820907
14	21.67439825269864	23.515516940887668	26.483556010427446	28.32652879598625
15	23.974962269118524	29.54207503161236	22.459793898536375	24.023168800732737
16	24.789652653398747	26.958204937090475	23.24296462753038	25.009177781980398
17	19.525870220600506	32.09813366558758	24.636504210655126	23.739491903156786
18	22.73605440663322	24.92018110823108	25.518683739195247	26.825080745940454
19	23.443207143466346	27.333474244733434	25.76564950884961	23.45766910295061
20	23.40352945975311	25.58951025872075	26.703081139009093	24.303879142517047
21	23.740233542104697	25.736725589881075	28.424054317636543	22.09898655037768
22	20.318682255917352	32.81233197242586	25.842409139958395	21.02657663169839
23	22.23062746363188	24.44516136209409	27.898973942515564	25.425237231758462
24	21.508271128366577	28.76706233104538	28.68399876887935	21.040667771708698
25	22.234335658371435	29.51166783474801	26.38677212772506	21.867224379155495
26	24.313891268313846	26.183933875471403	27.85892543932837	21.643249416886377
27	21.99033644450872	31.377260608218098	26.579598254181917	20.05280469309126
28	19.77320680972882	24.744783497050133	31.82817708854798	23.653832604673067
29	20.299770462745624	27.53927905277874	28.267197680153373	23.893752804322272
30	21.82346768122875	26.025593960092408	29.394488880978077	22.756449477700773
31	22.692297708706473	24.457398404734622	30.414613253829636	22.43569063272927
32	21.49900064151769	30.259239894242285	28.186729854305025	20.055029609934998
33	21.67254415532886	25.56355289554386	30.493968621256112	22.269934327871162
34	20.108798433658542	29.25617321719267	29.532804544763476	21.10222380438531
35	20.63387880877952	26.96376722919981	33.069680687350974	19.33267327466969
36	25.01325679619391	25.425237231758462	25.657741041928556	23.90376493011907
37	21.29245419452448	26.194687640216113	29.106362149714656	23.406496015544754
38	21.462289513596097	24.72513006493049	28.803402639493015	25.009177781980398
39	24.35987288308433	25.325486793264435	27.77252450189674	22.542115821754496
40	19.888902485602934	24.34726502096984	34.14283224497818	21.62100024844905
41	21.900598131811492	25.960700552150197	27.122107144578806	25.01659417145951
42	21.374405298268645	24.653932725931035	32.369573520523005	21.60208845527732
43	22.494280109614238	23.942330155410442	30.885183166279162	22.67820656869616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	6.0
2	9.0
3	52.5
4	96.0
5	96.0
6	117.5
7	139.0
8	149.0
9	159.0
10	225.5
11	292.0
12	292.0
13	496.5
14	701.0
15	1591.5
16	2482.0
17	2679.0
18	2876.0
19	2876.0
20	2863.5
21	2851.0
22	3037.5
23	3224.0
24	4202.0
25	5180.0
26	5180.0
27	6105.5
28	7031.0
29	7979.0
30	8927.0
31	10283.5
32	11640.0
33	11640.0
34	12842.0
35	14044.0
36	14839.0
37	15634.0
38	16193.0
39	16752.0
40	16752.0
41	17353.0
42	17954.0
43	18021.0
44	18088.0
45	18205.0
46	18322.0
47	18322.0
48	18676.5
49	19031.0
50	23626.5
51	28222.0
52	21922.5
53	15623.0
54	15623.0
55	20299.0
56	24975.0
57	20357.5
58	15740.0
59	11245.5
60	6751.0
61	6751.0
62	5801.5
63	4852.0
64	3854.5
65	2857.0
66	2368.5
67	1880.0
68	1880.0
69	1552.0
70	1224.0
71	977.5
72	731.0
73	684.5
74	638.0
75	638.0
76	541.0
77	444.0
78	323.0
79	202.0
80	128.5
81	55.0
82	55.0
83	41.5
84	28.0
85	19.0
86	10.0
87	7.0
88	4.0
89	4.0
90	2.5
91	1.0
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	269673.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.23866163762437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2839883116138	48.81541350075573
2	8.395783991108416	9.611268780992761
3	2.213873333369264	3.8015743991184867
4	1.0205323726512194	2.3365562867370055
5	0.6274814803239265	1.795810006806844
6	0.4219673024403894	1.4491706187915934
7	0.3086047147959493	1.2364884594986638
8	0.2395208956942592	1.0967884403027537
9	0.2011519282093732	1.0362300440878816
>10	1.198868827612589	12.568655102008567
>50	0.058385410266673946	2.221622678926678
>100	0.022705437325928758	2.4477580978580415
>500	0.0019461803422224648	0.7389318286919754
>1k	0.003243633903704108	4.133191047824311
>5k	0.0019461803422224648	6.710540707598684
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	6902	2.559396009240821	TruSeq Adapter, Index 11 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	6056	2.245682734274473	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	5114	1.8963707898083975	No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	3583	1.3286461751825358	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	2640	0.9789634112425049	RNA PCR Primer, Index 11 (95% over 22bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	1930	0.7156815847341039	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1522	0.5643872393602622	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1456	0.5399131540791996	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	920	0.3411539160390547	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.2002425159359669	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	530	0.19653432119641195	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	484	0.17947662539445922	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	477	0.17688088907677077	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	461	0.17094777749348283	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	312	0.1156956758741142	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	279	0.10345863323358288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	3.7081947395549425E-4	0.0
21	0.0	0.0	0.0	3.7081947395549425E-4	0.0
22	0.0	0.0	0.0	3.7081947395549425E-4	0.0
23	0.0	0.0	0.0	7.416389479109885E-4	0.0
24	0.0	0.0	0.0	0.0018540973697774713	0.0
25	0.0	0.0	0.0	0.0022249168437329654	0.0
26	0.0	0.0	0.0	0.0022249168437329654	0.0
27	0.0	0.0	0.0	0.004449833687465931	0.0
28	0.0	0.0	0.0	0.008899667374931862	0.0
29	0.0	0.0	0.0	0.02261998791128515	0.0
30	0.0	0.0	0.0	0.0385652252913714	0.0
31	0.0	0.0	0.0	0.06748914425989995	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	665	0.0	31.714285	1
CGTCTTC	295	0.0	31.355932	37
TTATACA	710	0.0	29.96479	2
TTGCACG	25	0.005488978	29.6	10
TTGGGCC	25	0.005488978	29.6	4
TATACAC	750	0.0	28.366665	3
CCGTCTT	1015	0.0	25.699507	37
GCCGTCT	1040	0.0	24.903845	36
CGACCTG	45	1.3195461E-4	24.666666	35
GCCGCTA	45	1.3195461E-4	24.666666	37
CGCCGCT	45	1.3195461E-4	24.666666	36
GGTATCA	245	0.0	23.408163	1
TTAAGGG	40	0.0019273767	23.125002	3
CTCGCCG	40	0.0019273767	23.125002	34
GTATCAA	630	0.0	22.611113	1
TGCCGTC	1220	0.0	21.22951	35
GCTGTGG	45	0.0038183315	20.555555	8
GCATATA	45	0.0038183315	20.555555	1
ATGCCGT	1275	0.0	20.313725	34
TCGCCGC	55	5.129075E-4	20.181818	35
>>END_MODULE