FastQCFastQC Report
Fri 10 Feb 2017
ERR1630860.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630860.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154057
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG73274.756031858338147No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT70824.5969998117579856No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT49383.205307126583018No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC47023.052117073550699No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27761.801930454312365No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24411.5844784722537764No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC23441.5215147640159161No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC14580.9464029547505144Illumina PCR Primer Index 7 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14550.9444556235679002No Hit
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG11750.7627047131905723No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9680.6283388615901907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9000.5841993547842682No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7580.4920256788071948No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7330.4757979189520762No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA6640.43100930175194896No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6220.4037466651953498No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT5720.37129114548511266No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5710.3706420350909079No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5520.3583089376010178No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5510.35765982720681305No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5490.3563616064184036No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT5440.35311605444737987RNA PCR Primer, Index 41 (95% over 21bp)
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG4600.29859078133418154No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4530.2940470085747483No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4040.2622405992587159No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3870.25120572255723533No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3840.24925839137462108No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3790.2460128394035974No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3740.24276728743257364No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3670.23822351467314046No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3670.23822351467314046No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3460.22459219639484085No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3410.22134664442381718No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3260.21160998851074603No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3210.20836443653972234No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT3200.20771532614551758No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3030.19668044944403695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.1882420143193753No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2880.18694379353096582No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2790.18110179998312312No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2770.17980357919471365No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2750.17850535840630416No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2730.17720713761789467No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2640.171365144070052No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2570.1668213713106188No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2500.16227759855118562No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2480.16097937776277613No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.1590320465801619No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2410.15643560500334291No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2380.1544882738207287No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2180.14150606593663384No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2150.13955873475401961No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2130.13826051396561012No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA2080.1350149619945864No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2040.13241852041776744No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2040.13241852041776744No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2000.12982207884094848No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2000.12982207884094848No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT1990.12917296844674372No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1970.12787474765833423No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG1940.12592741647572No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG1900.12333097489890105No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA1870.12138364371628682No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1840.1194363125336726No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC1830.11878720213946785No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1790.11619076056264889No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA1770.1148925397742394No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT1770.1148925397742394RNA PCR Primer, Index 41 (95% over 23bp)
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1770.1148925397742394No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1760.11424342938003466No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1730.11229609819742042No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC1700.1103487670148062No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC1690.10969965662060147No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1670.10840143583219197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1660.10775232543798724No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC1640.10645410464957775No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG1630.10580499425537301No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1630.10580499425537301No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1630.10580499425537301No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG1570.10191033189014456No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC1570.10191033189014456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG355.589209E-737.02
GATTACG355.589209E-737.01
TTACGGG355.589209E-737.03
GTCGCCT251.2286694E-436.99999621
GATCGTC251.2286694E-436.99999617
GGATCGT251.2286694E-436.99999616
GAGCCGT251.2286694E-436.99999632
CCGTTAC251.2286694E-436.99999635
TCGTCGC251.2286694E-436.99999619
TGAGCCG251.2286694E-436.99999631
CTTATAC7400.036.2500041
CGTCTTC2000.033.337
TTATACA8250.032.5151522
TATACAC8750.031.083
GGTGAGC303.585153E-430.83333429
GTGAGCC303.585153E-430.83333430
TCGCATG303.585153E-430.83333437
GCCGTTA303.585153E-430.83333434
ATCGTCG303.585153E-430.83333418
AGCCGTT303.585153E-430.83333433
Produced by FastQC (version 0.11.5)