FastQCFastQC Report
Fri 10 Feb 2017
ERR1630859.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630859.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713982
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT134401.8824003966486549No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG122981.7224523867548482No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT121561.702563930183114TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC64850.9082862032936404No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC47130.6601006748069279RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC33880.47452176665518175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25840.36191388578423545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25150.35224977660501244No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG16220.22717659548840166No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14090.19734391063080023No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA12190.17073259549960645No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT9590.13431711163586757No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT9200.12885478905630673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9060.12689395530979775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7990.11190758310433595No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7940.1112072853377256No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7660.10728561784470758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC16600.033.4337351
CGTCTTC5750.031.85217337
TATACAC19300.029.3316083
TTATACA19300.029.1398962
AATACGC358.8660914E-426.428575
CCGTCTT19200.025.82291637
GTATCAA13150.025.3231951
GGTATCA3600.025.1805551
GCCGTCT19700.025.16751336
TAGGGCC451.3225265E-424.6666664
TTACGGG901.4188117E-1024.6666663
GATTACG901.4188117E-1024.6666661
TGCCGTC23200.021.3706935
ATGCCGT24550.020.19551834
ATTACGG1101.7516868E-920.1818182
GGACCGT851.2439559E-619.5882366
ATAATAC1850.019.03
TATGCCG26600.018.70864733
AGGACCG609.232146E-418.55
GCACGCT500.007032334318.511
Produced by FastQC (version 0.11.5)