##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630859.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713982
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.486038863724854	31.0	31.0	34.0	28.0	34.0
2	31.493090862234624	31.0	31.0	34.0	28.0	34.0
3	30.986594900151545	31.0	31.0	34.0	27.0	34.0
4	35.108067150152245	37.0	35.0	37.0	32.0	37.0
5	35.296860425052735	37.0	35.0	37.0	33.0	37.0
6	35.477722127448594	37.0	35.0	37.0	33.0	37.0
7	35.475195453106664	37.0	35.0	37.0	33.0	37.0
8	35.50388105022255	37.0	35.0	37.0	33.0	37.0
9	37.275357081831196	39.0	37.0	39.0	34.0	39.0
10	37.12051564325151	39.0	37.0	39.0	33.0	39.0
11	37.167131664383696	39.0	37.0	39.0	33.0	39.0
12	37.097854007524	39.0	37.0	39.0	33.0	39.0
13	37.17414164502747	39.0	37.0	39.0	33.0	39.0
14	38.28685316996787	40.0	38.0	41.0	33.0	41.0
15	38.300525783563174	40.0	38.0	41.0	33.0	41.0
16	38.29984229294296	40.0	38.0	41.0	33.0	41.0
17	38.26523917969921	40.0	38.0	41.0	33.0	41.0
18	38.31940441075545	40.0	38.0	41.0	33.0	41.0
19	38.31903745472575	40.0	38.0	41.0	34.0	41.0
20	38.34523279298357	40.0	38.0	41.0	34.0	41.0
21	38.314949116364275	40.0	38.0	41.0	34.0	41.0
22	38.23324817712491	40.0	38.0	41.0	34.0	41.0
23	38.22909821255998	40.0	38.0	41.0	34.0	41.0
24	38.15844236969559	40.0	38.0	41.0	33.0	41.0
25	38.14785246686891	40.0	37.0	41.0	33.0	41.0
26	38.00820188744254	40.0	37.0	41.0	33.0	41.0
27	37.93697600219613	40.0	37.0	41.0	33.0	41.0
28	37.950599594947775	40.0	37.0	41.0	33.0	41.0
29	37.87043090722175	40.0	37.0	41.0	33.0	41.0
30	37.81400091318829	40.0	37.0	41.0	33.0	41.0
31	37.77796359011852	40.0	37.0	41.0	33.0	41.0
32	37.68150597634114	40.0	37.0	41.0	33.0	41.0
33	37.63032121257959	40.0	37.0	41.0	33.0	41.0
34	37.535905667089644	40.0	37.0	41.0	32.0	41.0
35	37.52590681557798	40.0	37.0	41.0	32.0	41.0
36	37.49355305876058	40.0	37.0	41.0	32.0	41.0
37	37.42917468507609	40.0	37.0	41.0	32.0	41.0
38	37.40495278592458	39.0	37.0	41.0	32.0	41.0
39	37.32198850951425	39.0	36.0	41.0	31.0	41.0
40	37.20831617603805	39.0	36.0	41.0	31.0	41.0
41	37.16954909227403	39.0	36.0	41.0	31.0	41.0
42	37.10627018608312	39.0	36.0	41.0	31.0	41.0
43	36.46169791395301	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	23.0
11	29.0
12	8.0
13	11.0
14	13.0
15	10.0
16	22.0
17	12.0
18	17.0
19	34.0
20	90.0
21	132.0
22	273.0
23	519.0
24	963.0
25	1593.0
26	2732.0
27	4183.0
28	6128.0
29	8445.0
30	12095.0
31	16061.0
32	20775.0
33	26548.0
34	35541.0
35	47169.0
36	65286.0
37	97770.0
38	169717.0
39	197769.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.20906129286172	22.870184402407904	15.562997386488735	24.357756918241634
2	13.47176819583687	22.116523945981832	41.354263832981786	23.057444025199516
3	14.46717144129684	28.77019308610021	34.60521413705108	22.157421335551877
4	11.316811908423462	19.368135331142803	37.62223697516184	31.6928157852719
5	12.58715205705466	38.07294861775227	35.36839864310305	13.971500682090024
6	27.535848242672785	39.98966360496483	17.8001686317022	14.674319520660184
7	23.852702168962242	33.950855903930346	22.54286522629422	19.653576700813186
8	24.743200808983982	34.99387939751983	22.55701124117975	17.705908552316448
9	23.355490754668885	14.931048681899545	19.734110943973377	41.9793496194582
10	14.200077873111647	28.46066147325843	33.56653248961459	23.772728164015337
11	31.17529573574684	22.921026020263817	25.320806406884206	20.582871837105138
12	20.153029067959697	26.675182287508648	29.309702485496835	23.86208615903482
13	29.157597810589063	20.73791776263267	26.60711334459412	23.497371082184145
14	21.436954993263136	22.689787697729074	26.84073267953534	29.03252462947245
15	23.917829861257005	28.89792739872994	22.82718611953803	24.357056620475028
16	24.656363885924296	27.59089164712836	23.521041146695577	24.23170332025177
17	20.441271628696523	31.034816003764803	25.099932491295302	23.42397987624338
18	23.34246521620993	26.19155664988753	25.939449453907805	24.526528679994733
19	22.914863399917646	29.151155071136248	26.377835855805888	21.55614567314022
20	21.7102111817945	25.690423568101156	27.66274219798258	24.93662305212176
21	23.74163494317784	25.724037860898452	26.49758677389626	24.036740422027446
22	20.563543618746692	30.074987884848635	28.093985562661246	21.267482933743427
23	22.460650268494163	25.96087856556608	26.208083677179538	25.370387488760223
24	23.11977052642784	29.856074803006237	26.11256306181388	20.91159160875204
25	23.541910020140563	27.152925423890238	26.872246079032802	22.432918476936393
26	24.477367776778685	28.43979259981344	25.796588709519284	21.286250913888587
27	21.22238375757372	29.19457353266609	28.93966514561992	20.643377564140273
28	20.892263390393595	25.69672624800065	27.698037205419745	25.71297315618601
29	21.341294318344158	28.364440560126162	28.226762019210565	22.067503102319105
30	21.759372085010547	25.11547910171405	30.19894058953867	22.926208223736733
31	22.983912759705426	26.47517724536473	25.808633831104988	24.73227616382486
32	23.583227588370576	25.967321305018892	29.368247378785462	21.08120372782507
33	22.338798457103962	25.83188371695645	28.86347274861271	22.96584507732688
34	21.115798437495624	30.303144897210295	26.662576927709665	21.91847973758442
35	23.372017781960892	25.596583667375366	30.736629214742106	20.294769335921632
36	24.978921037225028	25.754850962629313	25.581037056956617	23.685190943189045
37	21.941869682989207	26.704734853259605	28.23656618794311	23.116829275808072
38	21.885565742553734	24.988725205957575	28.442593790879883	24.683115260608812
39	24.14584681406534	25.56773139939102	27.109226843253754	23.177194943289887
40	20.90262779733943	25.1919516178279	32.004308231860186	21.901112352972486
41	22.454207529041348	26.001916014689446	26.5866646498091	24.957211806460105
42	22.480678784619222	25.007213066996087	30.487463269382143	22.02464487900255
43	22.851836600922713	24.61700715144079	29.673297085920936	22.85785916171556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	11.0
2	18.0
3	77.0
4	136.0
5	136.0
6	182.5
7	229.0
8	234.0
9	239.0
10	396.5
11	554.0
12	554.0
13	837.5
14	1121.0
15	2667.5
16	4214.0
17	4757.0
18	5300.0
19	5300.0
20	5424.0
21	5548.0
22	6584.5
23	7621.0
24	10019.5
25	12418.0
26	12418.0
27	15379.0
28	18340.0
29	21036.0
30	23732.0
31	27061.5
32	30391.0
33	30391.0
34	33892.0
35	37393.0
36	39818.0
37	42243.0
38	44323.5
39	46404.0
40	46404.0
41	47221.0
42	48038.0
43	49197.5
44	50357.0
45	50679.0
46	51001.0
47	51001.0
48	52562.5
49	54124.0
50	63547.0
51	72970.0
52	58021.5
53	43073.0
54	43073.0
55	50321.0
56	57569.0
57	48114.0
58	38659.0
59	29794.0
60	20929.0
61	20929.0
62	17965.0
63	15001.0
64	12631.0
65	10261.0
66	8424.0
67	6587.0
68	6587.0
69	5441.5
70	4296.0
71	3357.0
72	2418.0
73	1879.5
74	1341.0
75	1341.0
76	1001.5
77	662.0
78	533.5
79	405.0
80	296.5
81	188.0
82	188.0
83	143.0
84	98.0
85	75.0
86	52.0
87	41.5
88	31.0
89	31.0
90	23.0
91	15.0
92	8.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	713982.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.88023548040783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.64868768318739	46.44196582085974
2	11.392962432268245	12.960687719337155
3	3.0052035468104625	5.12810056227408
4	1.2291506758833812	2.796575195405967
5	0.6797300272482815	1.9331602006493138
6	0.3929239688025355	1.3409764722830786
7	0.26292624196670134	1.0468714589931263
8	0.19245396546336743	0.8757461499755694
9	0.15262758804344204	0.7813343833845896
>10	0.9184314142072844	9.967045988855922
>50	0.08261271614707257	3.2405114789640628
>100	0.0366347166035958	3.6354466462799566
>500	0.002950446974741499	1.2046831087043497
>1k	0.0017210940685992075	2.4404133093674134
>5k	2.4587058122845824E-4	0.9069386997849673
>10k+	7.376117436853748E-4	5.2995428048807325
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	13440	1.8824003966486549	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	12298	1.7224523867548482	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	12156	1.702563930183114	TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	6485	0.9082862032936404	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	4713	0.6601006748069279	RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	3388	0.47452176665518175	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2584	0.36191388578423545	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2515	0.35224977660501244	No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG	1622	0.22717659548840166	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1409	0.19734391063080023	No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA	1219	0.17073259549960645	No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT	959	0.13431711163586757	No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT	920	0.12885478905630673	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.12689395530979775	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	799	0.11190758310433595	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	794	0.1112072853377256	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	766	0.10728561784470758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.4005955332207254E-4	0.0	0.0	0.0
6	0.0	1.4005955332207254E-4	0.0	0.0	0.0
7	0.0	1.4005955332207254E-4	0.0	0.0	0.0
8	0.0	1.4005955332207254E-4	0.0	0.0	0.0
9	0.0	1.4005955332207254E-4	0.0	0.0	0.0
10	0.0	1.4005955332207254E-4	0.0	0.0	0.0
11	0.0	1.4005955332207254E-4	0.0	1.4005955332207254E-4	0.0
12	0.0	1.4005955332207254E-4	0.0	2.8011910664414507E-4	0.0
13	0.0	1.4005955332207254E-4	0.0	2.8011910664414507E-4	0.0
14	0.0	1.4005955332207254E-4	0.0	2.8011910664414507E-4	0.0
15	0.0	1.4005955332207254E-4	0.0	2.8011910664414507E-4	0.0
16	0.0	1.4005955332207254E-4	0.0	4.2017865996621764E-4	0.0
17	0.0	1.4005955332207254E-4	0.0	4.2017865996621764E-4	0.0
18	0.0	1.4005955332207254E-4	0.0	5.602382132882901E-4	0.0
19	0.0	1.4005955332207254E-4	0.0	9.804168732545078E-4	0.0
20	0.0	1.4005955332207254E-4	0.0	0.0012605359798986528	0.0
21	0.0	4.2017865996621764E-4	0.0	0.0016807146398648705	0.0
22	0.0	4.2017865996621764E-4	0.0	0.0022409528531531606	0.0
23	0.0	4.2017865996621764E-4	0.0	0.0032213697264076684	0.0
24	0.0	4.2017865996621764E-4	0.0	0.004201786599662177	0.0
25	0.0	4.2017865996621764E-4	0.0	0.005042143919594611	0.0
26	0.0	4.2017865996621764E-4	0.0	0.005602382132882902	0.0
27	0.0	4.2017865996621764E-4	0.0	0.0065827990061374094	0.0
28	0.0	4.2017865996621764E-4	0.0	0.01106470471244373	0.0
29	0.0	4.2017865996621764E-4	0.0	0.018207741931869432	0.0
30	0.0	4.2017865996621764E-4	0.0	0.03319411413733119	0.0
31	0.0	4.2017865996621764E-4	0.0	0.06344697765489886	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1660	0.0	33.433735	1
CGTCTTC	575	0.0	31.852173	37
TATACAC	1930	0.0	29.331608	3
TTATACA	1930	0.0	29.139896	2
AATACGC	35	8.8660914E-4	26.42857	5
CCGTCTT	1920	0.0	25.822916	37
GTATCAA	1315	0.0	25.323195	1
GGTATCA	360	0.0	25.180555	1
GCCGTCT	1970	0.0	25.167513	36
TAGGGCC	45	1.3225265E-4	24.666666	4
TTACGGG	90	1.4188117E-10	24.666666	3
GATTACG	90	1.4188117E-10	24.666666	1
TGCCGTC	2320	0.0	21.37069	35
ATGCCGT	2455	0.0	20.195518	34
ATTACGG	110	1.7516868E-9	20.181818	2
GGACCGT	85	1.2439559E-6	19.588236	6
ATAATAC	185	0.0	19.0	3
TATGCCG	2660	0.0	18.708647	33
AGGACCG	60	9.232146E-4	18.5	5
GCACGCT	50	0.0070323343	18.5	11
>>END_MODULE