FastQCFastQC Report
Fri 10 Feb 2017
ERR1630858.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630858.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643088
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT56350.8762408877167666RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG55120.8571144229094619No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT52810.8211939890030603No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC33330.5182805463637947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29900.4649441445027741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.46121215136964144No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC19440.30229144378374345RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC17930.27881098698778395RNA PCR Primer, Index 29 (95% over 24bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13230.2057261214639365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9960.15487771502500436No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG9530.14819122732814172No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8270.1285982633791954No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7940.12346677282113802No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6980.10853880028860746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGC250.00549456629.5999983
CGTCTTC2950.028.84745837
CTTATAC7900.027.6329121
TATACAC8350.027.2514973
CCGTCTT9400.025.97872437
GATTACG509.08296E-625.8999981
TTATACA8650.025.664742
TTAGACT659.3783456E-825.6153854
GCCGTCT9850.024.7918836
GTATCAA12850.023.4669271
GGTATCA4700.022.8297881
TTACGGG603.7230006E-521.5833343
TGCCGTC12250.019.93469435
TAGACTA656.896087E-519.9230775
ACGGTAC2000.019.4253
ATGCCGT12550.019.31075734
CACGGTA2050.018.9512182
TAGACTT801.614866E-518.55
TAGACTC701.2181378E-418.55
TACACCG609.230784E-418.55
Produced by FastQC (version 0.11.5)