##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630858.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643088
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52785466374742	31.0	31.0	34.0	28.0	34.0
2	31.518630420720026	31.0	31.0	34.0	28.0	34.0
3	31.0059587490359	31.0	31.0	34.0	27.0	34.0
4	35.13909604906327	37.0	35.0	37.0	32.0	37.0
5	35.32501617197024	37.0	35.0	37.0	33.0	37.0
6	35.51184596820342	37.0	35.0	37.0	33.0	37.0
7	35.51891965018785	37.0	35.0	37.0	33.0	37.0
8	35.540479685517376	37.0	35.0	37.0	33.0	37.0
9	37.30756443908143	39.0	37.0	39.0	34.0	39.0
10	37.15063879282462	39.0	37.0	39.0	33.0	39.0
11	37.2045023387157	39.0	37.0	39.0	33.0	39.0
12	37.13796401114622	39.0	37.0	39.0	33.0	39.0
13	37.202314457741394	39.0	37.0	39.0	33.0	39.0
14	38.359404933694925	40.0	38.0	41.0	34.0	41.0
15	38.36659990545618	40.0	38.0	41.0	34.0	41.0
16	38.37790006966387	40.0	38.0	41.0	34.0	41.0
17	38.35694803821561	40.0	38.0	41.0	34.0	41.0
18	38.39613707610778	40.0	38.0	41.0	34.0	41.0
19	38.41944492822133	40.0	38.0	41.0	34.0	41.0
20	38.41413927798373	40.0	38.0	41.0	34.0	41.0
21	38.366728970218695	40.0	38.0	41.0	34.0	41.0
22	38.30426007016147	40.0	38.0	41.0	34.0	41.0
23	38.297147513248575	40.0	38.0	41.0	34.0	41.0
24	38.27242150374443	40.0	38.0	41.0	34.0	41.0
25	38.25572705446222	40.0	38.0	41.0	34.0	41.0
26	38.13477160202025	40.0	38.0	41.0	33.0	41.0
27	38.06081438310154	40.0	37.0	41.0	33.0	41.0
28	38.03621588336277	40.0	37.0	41.0	33.0	41.0
29	37.960960241833156	40.0	37.0	41.0	33.0	41.0
30	37.880591769711145	40.0	37.0	41.0	33.0	41.0
31	37.84130476699923	40.0	37.0	41.0	33.0	41.0
32	37.7523122807454	40.0	37.0	41.0	33.0	41.0
33	37.7231638593785	40.0	37.0	41.0	33.0	41.0
34	37.654383225934865	40.0	37.0	41.0	33.0	41.0
35	37.62238760480681	40.0	37.0	41.0	33.0	41.0
36	37.5758838603737	40.0	37.0	41.0	32.0	41.0
37	37.51898807006195	40.0	37.0	41.0	32.0	41.0
38	37.48073669544448	40.0	37.0	41.0	32.0	41.0
39	37.39430217948399	40.0	36.0	41.0	32.0	41.0
40	37.30333640186102	39.0	36.0	41.0	31.0	41.0
41	37.2306294006419	39.0	36.0	41.0	31.0	41.0
42	37.15612015773891	39.0	36.0	41.0	31.0	41.0
43	36.57563661582863	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	6.0
11	6.0
12	3.0
13	6.0
14	6.0
15	1.0
16	6.0
17	3.0
18	11.0
19	19.0
20	53.0
21	115.0
22	242.0
23	409.0
24	853.0
25	1377.0
26	2367.0
27	3522.0
28	5369.0
29	7615.0
30	10429.0
31	13778.0
32	17937.0
33	23558.0
34	30755.0
35	41554.0
36	57851.0
37	86997.0
38	151472.0
39	186761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.326169979847236	22.66703779265046	15.543129400641902	23.4636628268604
2	13.931841365411888	22.93605229766377	40.31361182295425	22.818494513970094
3	15.059369790759586	28.26658248948822	33.922729082178485	22.751318637573707
4	11.76728534819496	18.734139029184185	39.24579528773667	30.252780334884182
5	12.739003060234369	38.34389694722962	35.107014903092576	13.810085089443437
6	27.720778493767572	40.30692533525738	18.057404274376136	13.91489189659891
7	24.64266165750255	33.63645410892444	23.191071828427837	18.529812405145172
8	24.860205757221408	35.28428457691638	22.04612743512552	17.809382230736695
9	24.521838379817382	15.164332097628941	20.56732515612171	39.746504366431964
10	14.566435697758315	28.782530540143807	33.79521931679646	22.85581444530142
11	32.111312915184236	23.581687109695718	25.12953126166248	19.177468713457568
12	21.02729330978031	26.504615231507973	30.546052795262856	21.92203866344886
13	28.432811683626504	21.698585574602543	26.760567760555322	23.108034981215635
14	21.516028910506805	22.14922374542831	28.056813375463392	28.2779339686015
15	23.314383101535093	29.211243251312418	24.043521259920883	23.430852387231607
16	23.18702883586694	28.200339611375114	24.86160525464633	23.75102629811161
17	21.140808100913095	29.43345234244769	26.48144577413978	22.94429378249944
18	23.027330629711642	26.42842037170652	27.675683576742216	22.868565421839623
19	23.634246012987337	27.294553778021047	28.110460776752173	20.960739432239446
20	23.642798497250762	26.186929316050055	27.13532207100739	23.03495011569179
21	23.222171771203943	26.528873186873337	27.573675764436594	22.67527927748613
22	21.866369765879632	29.41541437563755	27.48333043067201	21.234885427810813
23	23.318892593237628	25.896300350807355	27.787798870450082	22.99700818550494
24	22.1700607070883	27.182438484313188	28.00067797875252	22.64682282984599
25	22.64184683900182	27.26827432637524	28.288818948573137	21.80105988604981
26	22.583378946582737	27.489239419799468	27.99399149105566	21.933390142562136
27	22.64013634214913	28.552390963600626	27.76214141765979	21.045331276590453
28	21.77462493469012	26.612376533227177	28.270780981762993	23.342217550319706
29	21.830760331400988	27.391896598910257	28.074851342273533	22.70249172741522
30	23.250317219416317	26.64472047371433	28.808343492647975	21.296618814221382
31	22.47173015201652	26.383791953822804	26.953698405194938	24.19077948896574
32	21.91301968004379	27.32783071679148	29.01749060781728	21.74165899534745
33	21.974442067026597	27.378990122658177	29.127584405244693	21.518983405070536
34	21.402203119946257	27.765873410792924	27.66371009877342	23.168213370487397
35	21.603419749707662	26.657004951110892	30.630800139327746	21.108775159853707
36	22.82331500510039	27.441034508496504	27.0210297813052	22.7146207050979
37	22.337222899509865	26.588274077575697	28.434055681337544	22.64044734157689
38	22.107238822680568	26.029408105889086	28.248233523250317	23.61511954818003
39	23.268199686512578	26.21880675739557	27.92168412410121	22.591309431990645
40	21.636385689050332	26.22316074938422	30.21250590898913	21.927947652576318
41	22.452292687781455	26.088964496305326	27.613483691190012	23.84525912472321
42	22.09666484213669	26.03391759759162	29.569981091234794	22.299436469036895
43	22.624119871619435	25.644857313462545	29.07502550195308	22.655997312964942
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	12.5
2	20.0
3	85.0
4	150.0
5	150.0
6	201.5
7	253.0
8	257.0
9	261.0
10	390.5
11	520.0
12	520.0
13	847.5
14	1175.0
15	3001.0
16	4827.0
17	5430.5
18	6034.0
19	6034.0
20	5966.0
21	5898.0
22	6911.5
23	7925.0
24	10219.5
25	12514.0
26	12514.0
27	14743.5
28	16973.0
29	19591.5
30	22210.0
31	25375.5
32	28541.0
33	28541.0
34	31962.0
35	35383.0
36	37689.0
37	39995.0
38	41360.5
39	42726.0
40	42726.0
41	43792.5
42	44859.0
43	45190.5
44	45522.0
45	46680.5
46	47839.0
47	47839.0
48	48276.0
49	48713.0
50	51347.0
51	53981.0
52	47578.0
53	41175.0
54	41175.0
55	43005.0
56	44835.0
57	38430.0
58	32025.0
59	26117.5
60	20210.0
61	20210.0
62	17281.5
63	14353.0
64	11903.0
65	9453.0
66	7779.0
67	6105.0
68	6105.0
69	4975.5
70	3846.0
71	3015.5
72	2185.0
73	1673.0
74	1161.0
75	1161.0
76	924.5
77	688.0
78	532.5
79	377.0
80	287.5
81	198.0
82	198.0
83	143.5
84	89.0
85	65.5
86	42.0
87	27.0
88	12.0
89	12.0
90	10.5
91	9.0
92	5.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	643088.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.50901658491687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15752224136855	48.296043370523606
2	11.660018780502497	13.877525019787305
3	3.137268724895259	5.600873297433989
4	1.3149689699650302	3.1301004096920013
5	0.6640058327776986	1.9757167057624803
6	0.4258603993064209	1.5205520139111073
7	0.27547971808330246	1.1475468978559227
8	0.19742050073831052	0.9398639882110972
9	0.1475406999369457	0.7902001763548151
>10	0.9064651884490222	10.03812886722071
>50	0.07502271385003006	3.069012307902735
>100	0.03345957453230558	3.739777345293914
>500	0.002614029260329224	1.0870414745174515
>1k	0.0015684175561975341	2.232106198891086
>5k	7.842087780987671E-4	2.5555119266417705
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	5635	0.8762408877167666	RNA PCR Primer, Index 29 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	5512	0.8571144229094619	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	5281	0.8211939890030603	No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	3333	0.5182805463637947	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2990	0.4649441445027741	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2966	0.46121215136964144	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	1944	0.30229144378374345	RNA PCR Primer, Index 29 (95% over 21bp)
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	1793	0.27881098698778395	RNA PCR Primer, Index 29 (95% over 24bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1323	0.2057261214639365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	996	0.15487771502500436	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	953	0.14819122732814172	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	827	0.1285982633791954	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	794	0.12346677282113802	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	698	0.10853880028860746	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.5549971388052647E-4	0.0
13	0.0	0.0	0.0	1.5549971388052647E-4	0.0
14	0.0	0.0	0.0	1.5549971388052647E-4	0.0
15	0.0	0.0	0.0	1.5549971388052647E-4	0.0
16	0.0	0.0	0.0	4.6649914164157937E-4	0.0
17	0.0	0.0	0.0	4.6649914164157937E-4	0.0
18	0.0	0.0	0.0	4.6649914164157937E-4	0.0
19	0.0	0.0	0.0	4.6649914164157937E-4	0.0
20	0.0	0.0	0.0	9.329982832831587E-4	0.0
21	0.0	0.0	0.0	0.0012439977110442117	0.0
22	0.0	0.0	0.0	0.0018659965665663175	0.0
23	0.0	0.0	0.0	0.002332495708207897	0.0
24	0.0	0.0	0.0	0.0024879954220884235	0.0
25	0.0	0.0	0.0	0.0024879954220884235	0.0
26	0.0	0.0	0.0	0.0024879954220884235	0.0
27	0.0	0.0	0.0	0.0029544945637300026	0.0
28	0.0	0.0	0.0	0.00746398626626527	0.0
29	0.0	0.0	0.0	0.01850446595178265	0.0
30	0.0	0.0	0.0	0.03187744134550793	0.0
31	0.0	0.0	0.0	0.064687880974299	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACGC	25	0.005494566	29.599998	3
CGTCTTC	295	0.0	28.847458	37
CTTATAC	790	0.0	27.632912	1
TATACAC	835	0.0	27.251497	3
CCGTCTT	940	0.0	25.978724	37
GATTACG	50	9.08296E-6	25.899998	1
TTATACA	865	0.0	25.66474	2
TTAGACT	65	9.3783456E-8	25.615385	4
GCCGTCT	985	0.0	24.79188	36
GTATCAA	1285	0.0	23.466927	1
GGTATCA	470	0.0	22.829788	1
TTACGGG	60	3.7230006E-5	21.583334	3
TGCCGTC	1225	0.0	19.934694	35
TAGACTA	65	6.896087E-5	19.923077	5
ACGGTAC	200	0.0	19.425	3
ATGCCGT	1255	0.0	19.310757	34
CACGGTA	205	0.0	18.951218	2
TAGACTT	80	1.614866E-5	18.5	5
TAGACTC	70	1.2181378E-4	18.5	5
TACACCG	60	9.230784E-4	18.5	5
>>END_MODULE