FastQCFastQC Report
Fri 10 Feb 2017
ERR1630857.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630857.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855007
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT190222.2247771070880122No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG157711.8445463019601012No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT146561.7141380129051575TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC84110.9837346360906987No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC56450.6602285127490184RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC40010.46794938520971174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36510.4270140478382048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32260.37730685245851786No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG20500.2397641188902547No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG17700.2070158489930492No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15630.18280552089047225No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA14700.17192841696032898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12860.15040812531359393No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT12830.15005725099326672No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG11440.13380007415143969No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT10830.12666562963811992No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA9300.10877103930143261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23000.033.31
CGTCTTC7750.032.22580737
TTATACA25100.030.6613542
TATACAC25950.029.9421963
GGTATCA6100.029.7213121
CGATTTG250.00549556629.635
TCGCCGA509.087533E-625.9000021
CCGTCTT24200.025.07437937
GCCGTCT24650.024.54158436
GTATCAA18450.024.4661251
AGCAACG400.001930784423.1250022
GCAACGC400.001930784423.1250023
ACGATTT400.001930784423.12500234
ACAATCG400.001930784423.12500237
GATTACG855.176844E-821.7647061
TGCCGTC29400.020.63945635
GGTACGA450.003825018420.55555531
GGGTACG450.003825018420.55555530
TTAACGG1101.7535058E-920.18181835
ATGCCGT31100.019.57073834
Produced by FastQC (version 0.11.5)