##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630857.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855007
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.473204312947146	31.0	31.0	34.0	28.0	34.0
2	31.47924987748638	31.0	31.0	34.0	28.0	34.0
3	30.965093853032783	31.0	31.0	34.0	27.0	34.0
4	35.102669334870946	37.0	35.0	37.0	32.0	37.0
5	35.292298191710714	37.0	35.0	37.0	33.0	37.0
6	35.48620888483954	37.0	35.0	37.0	33.0	37.0
7	35.482225291722756	37.0	35.0	37.0	33.0	37.0
8	35.50823794425075	37.0	35.0	37.0	33.0	37.0
9	37.30021157721516	39.0	37.0	39.0	34.0	39.0
10	37.12608785659065	39.0	37.0	39.0	33.0	39.0
11	37.179352917578456	39.0	37.0	39.0	33.0	39.0
12	37.104868147278324	39.0	37.0	39.0	33.0	39.0
13	37.19315982208333	39.0	37.0	39.0	33.0	39.0
14	38.28626198382002	40.0	38.0	41.0	33.0	41.0
15	38.299978830582674	40.0	38.0	41.0	34.0	41.0
16	38.309051270925266	40.0	38.0	41.0	34.0	41.0
17	38.252748807904496	40.0	38.0	41.0	33.0	41.0
18	38.31841025862946	40.0	38.0	41.0	34.0	41.0
19	38.34208959692727	40.0	38.0	41.0	34.0	41.0
20	38.342696609501445	40.0	38.0	41.0	34.0	41.0
21	38.314617307226726	40.0	38.0	41.0	34.0	41.0
22	38.22882034883925	40.0	38.0	41.0	34.0	41.0
23	38.218739729616246	40.0	38.0	41.0	34.0	41.0
24	38.17767924707049	40.0	38.0	41.0	33.0	41.0
25	38.133947441365976	40.0	37.0	41.0	33.0	41.0
26	38.020665327886206	40.0	37.0	41.0	33.0	41.0
27	37.94540395575709	40.0	37.0	41.0	33.0	41.0
28	37.93966949978187	40.0	37.0	41.0	33.0	41.0
29	37.89992245677521	40.0	37.0	41.0	33.0	41.0
30	37.78809881088693	40.0	37.0	41.0	33.0	41.0
31	37.74664885784561	40.0	37.0	41.0	33.0	41.0
32	37.64835375616808	40.0	37.0	41.0	32.0	41.0
33	37.55993225786455	40.0	37.0	41.0	32.0	41.0
34	37.51478759823019	40.0	37.0	41.0	32.0	41.0
35	37.510386464672216	40.0	37.0	41.0	32.0	41.0
36	37.44283964926603	39.0	36.0	41.0	32.0	41.0
37	37.38816524309158	39.0	36.0	41.0	32.0	41.0
38	37.35891051184376	39.0	36.0	41.0	32.0	41.0
39	37.233442533219026	39.0	36.0	41.0	31.0	41.0
40	37.12465044145837	39.0	36.0	41.0	31.0	41.0
41	37.08052565651509	39.0	36.0	41.0	31.0	41.0
42	37.00970635328132	39.0	36.0	41.0	31.0	41.0
43	36.33841945153665	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	13.0
10	21.0
11	17.0
12	22.0
13	11.0
14	17.0
15	10.0
16	18.0
17	16.0
18	33.0
19	45.0
20	75.0
21	165.0
22	359.0
23	640.0
24	1216.0
25	2072.0
26	3226.0
27	5041.0
28	7558.0
29	10659.0
30	14482.0
31	18874.0
32	24693.0
33	32369.0
34	42263.0
35	56763.0
36	79471.0
37	118300.0
38	202004.0
39	234549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.65594901562209	22.57677422524026	15.615310751841799	24.151966007295847
2	13.537900859291211	22.108240049496672	41.53521550115964	22.818643590052478
3	14.20222290577738	29.016136709991848	34.562290133297154	22.219350250933616
4	10.90131425824584	19.699253924236878	37.78355031011442	31.615881507402865
5	12.383173471094388	38.060390148852576	35.556667957104445	13.999768422948582
6	27.11346222896421	40.48376212124579	17.542078602865242	14.860697046924761
7	24.406817721960174	33.2910724707517	22.313150652567757	19.98895915472037
8	24.958626069728084	34.520536089178215	22.54823644718698	17.972601393906718
9	23.479456893335378	14.550991980182618	19.51820277494804	42.451348351533966
10	14.384794510454299	28.71964790931536	33.37458055898958	23.52097702124076
11	30.844776709430448	23.26133002419863	25.128800115086776	20.76509315128414
12	20.268021197487272	26.88094951269405	28.666315012625628	24.184714277193052
13	29.183854635108254	20.708017595177584	26.703874939035586	23.40425283067858
14	21.785786549115972	22.750924846229328	26.46200557422337	29.00128303043133
15	23.853722835017724	29.165258296130908	22.964607307308597	24.01641156154277
16	25.178741226680017	27.198724688803715	23.282382483418264	24.340151601098004
17	20.924507050819464	30.85951343088419	24.433718086518592	23.78226143177775
18	24.26050313038373	24.81102493897711	25.90832589674704	25.02014603389212
19	25.324587985829357	26.918610023075836	25.624702487815888	22.13209950327892
20	22.187537645890618	25.550083215692972	26.228791109312553	26.033588029103854
21	24.375239033130722	25.190904869784692	27.124807165321453	23.309048931763133
22	20.902869801065957	30.444195193723562	25.830665713847957	22.822269291362527
23	22.646013424451496	26.575571895902605	27.261648150249062	23.516766529396836
24	24.10202489570261	27.466324837106598	27.180128349826376	21.251521917364418
25	22.536891510829733	28.80549515968875	26.018032600902686	22.63958072857883
26	25.298506328018366	27.538604947094004	26.74305590480546	20.419832820082178
27	21.031406760412487	29.209819334812465	25.833706624624124	23.925067280150923
28	20.505095279921683	26.22458061746863	30.097063532813184	23.173260569796504
29	23.493725782362016	25.44213088313897	27.232291665448354	23.831851669050664
30	21.555379078767775	27.305624398396738	27.847959139515815	23.29103738331967
31	21.84789130381389	25.477101357064914	29.8617438219804	22.813263517140793
32	23.462731884066446	25.914875550726485	27.495447405693756	23.126945159513316
33	20.486732857157893	29.32923356183049	29.27742112052884	20.90661246048278
34	21.274094831972135	28.03672952385185	29.244321976311305	21.44485366786471
35	23.441094634312936	25.62902993776659	30.628404211895344	20.30147121602513
36	22.890572825719556	27.70234629658003	25.174764651049642	24.232316226650777
37	21.778301230282327	26.92796667161789	28.33450486370287	22.959227234396913
38	21.61374117404887	24.62073409925299	28.734969421302985	25.030555305395158
39	24.155591708605893	25.341079078884732	27.150537948812115	23.352791263697256
40	20.91316211446222	24.831024775235758	32.64920638076647	21.606606729535546
41	22.404962766386706	25.859554366221566	26.208557356840352	25.526925510551372
42	22.304495752666355	25.497569025750664	30.229343151576536	21.968592070006444
43	23.212207619352824	24.025885168191603	29.853673712612878	22.90823349984269
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	14.5
2	25.0
3	102.5
4	180.0
5	180.0
6	277.0
7	374.0
8	387.0
9	400.0
10	568.5
11	737.0
12	737.0
13	1149.0
14	1561.0
15	3466.5
16	5372.0
17	6191.0
18	7010.0
19	7010.0
20	6804.5
21	6599.0
22	7742.5
23	8886.0
24	11474.5
25	14063.0
26	14063.0
27	17121.0
28	20179.0
29	23576.5
30	26974.0
31	30924.5
32	34875.0
33	34875.0
34	40772.5
35	46670.0
36	49095.0
37	51520.0
38	53079.0
39	54638.0
40	54638.0
41	55121.5
42	55605.0
43	56921.5
44	58238.0
45	58484.0
46	58730.0
47	58730.0
48	59257.5
49	59785.0
50	75357.5
51	90930.0
52	72784.5
53	54639.0
54	54639.0
55	64315.0
56	73991.0
57	60869.0
58	47747.0
59	37235.5
60	26724.0
61	26724.0
62	22839.0
63	18954.0
64	14926.0
65	10898.0
66	8986.5
67	7075.0
68	7075.0
69	5713.5
70	4352.0
71	4028.5
72	3705.0
73	3075.0
74	2445.0
75	2445.0
76	1513.0
77	581.0
78	419.5
79	258.0
80	199.5
81	141.0
82	141.0
83	107.0
84	73.0
85	55.5
86	38.0
87	28.5
88	19.0
89	19.0
90	15.5
91	12.0
92	6.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	855007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.30933245971464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.06119953532432	39.97073637470573
2	11.66129639427577	11.500214816332308
3	2.9805626186278578	4.409086592367561
4	1.1849755910273951	2.337214214984667
5	0.605223769510515	1.4921590031657843
6	0.38402429188455356	1.1361588888685168
7	0.2737234856155137	0.9447985647973096
8	0.2025037260106013	0.7988258840150163
9	0.15655361550264046	0.694759884713103
>10	1.2362211651911563	12.183587891337968
>50	0.15972969619344204	5.468955024442568
>100	0.08639127359741686	7.75897931266779
>500	0.0037974186194489976	1.236189059820041
>1k	0.0026107253008711855	2.6363373047965597
>5k	4.746773274311247E-4	1.6449752366591397
>10k+	7.120159911466871E-4	5.787021946325967
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	19022	2.2247771070880122	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	15771	1.8445463019601012	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	14656	1.7141380129051575	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	8411	0.9837346360906987	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5645	0.6602285127490184	RNA PCR Primer, Index 8 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	4001	0.46794938520971174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3651	0.4270140478382048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3226	0.37730685245851786	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	2050	0.2397641188902547	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1770	0.2070158489930492	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1563	0.18280552089047225	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1470	0.17192841696032898	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1286	0.15040812531359393	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1283	0.15005725099326672	No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG	1144	0.13380007415143969	No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT	1083	0.12666562963811992	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	930	0.10877103930143261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.16958106775734E-4	0.0
2	0.0	0.0	0.0	2.33916213551468E-4	0.0
3	0.0	0.0	0.0	2.33916213551468E-4	0.0
4	0.0	0.0	0.0	2.33916213551468E-4	0.0
5	0.0	1.16958106775734E-4	0.0	2.33916213551468E-4	0.0
6	0.0	1.16958106775734E-4	0.0	3.50874320327202E-4	0.0
7	0.0	1.16958106775734E-4	0.0	5.8479053387867E-4	0.0
8	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
9	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
10	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
11	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
12	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
13	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
14	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
15	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
16	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
17	0.0	1.16958106775734E-4	0.0	7.01748640654404E-4	0.0
18	0.0	1.16958106775734E-4	0.0	9.35664854205872E-4	0.0
19	0.0	1.16958106775734E-4	0.0	0.001052622960981606	0.0
20	0.0	1.16958106775734E-4	0.0	0.001403497281308808	0.0
21	0.0	1.16958106775734E-4	0.0	0.001637413494860276	0.0
22	0.0	1.16958106775734E-4	0.0	0.002222204028738946	0.0
23	0.0	1.16958106775734E-4	0.0	0.00233916213551468	0.0
24	0.0	1.16958106775734E-4	0.0	0.003157868882944818	0.0
25	0.0	1.16958106775734E-4	0.0	0.004210491843926424	0.0
26	0.0	1.16958106775734E-4	0.0	0.004912240484580828	0.0
27	0.0	1.16958106775734E-4	0.0	0.005730947232010966	0.0
28	0.0	1.16958106775734E-4	0.0	0.011461894464021932	0.0
29	0.0	1.16958106775734E-4	0.0	0.023625537568698268	0.0
30	0.0	1.16958106775734E-4	0.0	0.043157541400245844	0.0
31	0.0	1.16958106775734E-4	0.0	0.08479462741240715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2300	0.0	33.3	1
CGTCTTC	775	0.0	32.225807	37
TTATACA	2510	0.0	30.661354	2
TATACAC	2595	0.0	29.942196	3
GGTATCA	610	0.0	29.721312	1
CGATTTG	25	0.005495566	29.6	35
TCGCCGA	50	9.087533E-6	25.900002	1
CCGTCTT	2420	0.0	25.074379	37
GCCGTCT	2465	0.0	24.541584	36
GTATCAA	1845	0.0	24.466125	1
AGCAACG	40	0.0019307844	23.125002	2
GCAACGC	40	0.0019307844	23.125002	3
ACGATTT	40	0.0019307844	23.125002	34
ACAATCG	40	0.0019307844	23.125002	37
GATTACG	85	5.176844E-8	21.764706	1
TGCCGTC	2940	0.0	20.639456	35
GGTACGA	45	0.0038250184	20.555555	31
GGGTACG	45	0.0038250184	20.555555	30
TTAACGG	110	1.7535058E-9	20.181818	35
ATGCCGT	3110	0.0	19.570738	34
>>END_MODULE