FastQCFastQC Report
Fri 10 Feb 2017
ERR1630856.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630856.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236038
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG67532.8609800116930324No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT61512.6059363322854794TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT60062.544505545717215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51622.186936001830214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46331.9628195460053042No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC42681.8081834280920868No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25351.0739796134520714No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC22240.9422211677780696No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC19060.8074970979249104RNA PCR Primer, Index 6 (95% over 22bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA16000.6778569552360213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15990.6774332946389988No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13380.5668578788161228No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11820.5007668256806107No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11120.4711105838890348No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10690.45289317821706676No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG10580.44823291164981904No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10180.4312864877689186No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC9750.41306908209695054No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9750.41306908209695054No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG9340.39569899761902744No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7650.3241003567222227No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6830.28936018776637656No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6740.285547242393174No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6690.2834289394080614No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6570.27834501224379127No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6370.269871800303341No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6230.2639405519450258No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6060.25673832179564304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.255043679407553No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA5920.2508070734373279No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5880.24911243104923783No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5770.24445216448199017No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5640.2389445767206975No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5610.23767359492962997No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG5340.22623475881002214No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5250.2224218134368195No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5190.2198798498546844No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT5140.21776154686957183No Hit
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT4980.21098297731721163No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4830.20462806836187394No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4830.20462806836187394No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4550.19276557164524358No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4510.19107092925715352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.190647268660131No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4340.1838686991077708No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3930.16649861462984772No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3870.16395665104771265No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3850.1631093298536676No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3810.16141468746557758No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3750.1588727238834425No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3720.15760174209237496No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3670.1554834391072624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3510.14870486955490217No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3460.1465865665697896No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3450.1461629059727671No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3280.13896067582338437No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3260.13811335462933935No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3250.13768969403231685No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3230.1368423728382718No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3230.1368423728382718No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3220.1364187122412493No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3190.13514773045018175No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC3170.13430040925613673No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3170.13430040925613673No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3160.13387674865911423No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3130.13260576686804668No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3050.12921648209186656No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3010.1275218397037765No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2990.12667451850973147No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2940.12455621552461892No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2870.12159059134546131No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2800.11862496716630373No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2790.11820130656928121No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2760.11693032477821369No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2700.11438836119607859No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2650.11227005821096603No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2640.11184639761394352No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2630.11142273701692101No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2610.11057541582287597No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA2590.10972809462883096No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2550.1080334522407409No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2550.1080334522407409No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2510.10633880985265086No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT2460.10422050686753827No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG2440.10337318567349325No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2440.10337318567349325No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2370.10040756149433565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTT308.284005E-637.03
TGACATC200.00183897437.017
ACATCCA200.00183897437.019
GACATCC200.00183897437.018
TTGACAT200.00183897437.016
GATTACG403.8113285E-837.01
ACAGACA251.2306552E-436.99999617
GACAGAC251.2306552E-436.99999616
TAAGAGA251.2306552E-436.99999611
GACACAT251.2306552E-436.99999620
ACTCAGA251.2306552E-436.9999961
CAGACAC251.2306552E-436.99999618
TGTGTAT251.2306552E-436.9999965
CTTATAC7750.034.8516121
TTACGGG507.2795956E-933.33
TTATACA8150.033.14112
ATTACGG451.0737858E-732.888892
TATACAC8600.031.4069773
ATAAGAG303.5908932E-430.83333210
GTGTATA303.5908932E-430.8333326
Produced by FastQC (version 0.11.5)