FastQCFastQC Report
Fri 10 Feb 2017
ERR1630855.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630855.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634248
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT121391.9139201069613148No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG97141.5315775532599236No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT81491.2848286474691288Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC49980.7880198282060014No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC29580.46637908199947026Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC22300.3515974823728258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20010.3154917319408181No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18340.2891613375209697No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC15180.2393385552654482No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT14920.2352392124216395No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC13640.2150578322675042No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12630.19913346198963183No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12160.19172311146428528No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG12160.19172311146428528No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT11730.18494342906875544No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG11310.1783214137056798No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT11210.17674474338113796No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA10820.1705957291154249No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA9260.1459996720525725No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA8910.14048132591667611No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8890.14016599185176776No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8440.13307097539132956No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8360.13180963913169613No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT8300.13086363693697103No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA8050.1269219611256165No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG7980.12581829189843718No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG7790.12282261828180775No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC7700.12140361498972012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7350.11588526885382375No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT7260.1144662655617361No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG7160.11288959523719427No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC7020.11068225678283573No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC6660.10500624361448518No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT6520.10279890516012664No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT6500.10248357109521827No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA6440.10153756890049319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT200.001841314537.011
ATCGGCG200.001841314537.010
CTTATAC13800.033.3804321
TTATACA15650.029.9073492
CGGCGTA250.00549450829.612
TATACAC15900.029.3207533
CGTCTTC3950.028.5696237
TAAGGGT405.9359976E-527.754
GGTATCA3250.027.3230761
GTATCAA11000.026.2363621
CCGTCTT13650.026.0219837
GCCGTCT13950.025.46236636
TCATATA603.722896E-521.5833342
TGCCGTC16600.021.3975935
ATGCCGT17150.020.7113734
GACCCTT450.003823946520.5555556
CACGAGG759.25504E-619.73333214
TATGCCG18300.019.40983633
TACACAT25050.018.7584825
TAATCAG500.007031488718.55
Produced by FastQC (version 0.11.5)