##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630855.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	634248
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.522737162750218	31.0	31.0	34.0	28.0	34.0
2	31.535121908149495	31.0	31.0	34.0	30.0	34.0
3	31.007512834096442	31.0	31.0	34.0	27.0	34.0
4	35.13816677388025	37.0	35.0	37.0	32.0	37.0
5	35.33826042809753	37.0	35.0	37.0	33.0	37.0
6	35.52347189112145	37.0	35.0	37.0	33.0	37.0
7	35.520419457373144	37.0	35.0	37.0	33.0	37.0
8	35.54561937917029	37.0	35.0	37.0	33.0	37.0
9	37.32650635082807	39.0	37.0	39.0	34.0	39.0
10	37.1641439941474	39.0	37.0	39.0	33.0	39.0
11	37.22334323482297	39.0	37.0	39.0	33.0	39.0
12	37.15732174165311	39.0	37.0	39.0	33.0	39.0
13	37.223073624197475	39.0	37.0	39.0	34.0	39.0
14	38.364663349352305	40.0	38.0	41.0	34.0	41.0
15	38.38767169939834	40.0	38.0	41.0	34.0	41.0
16	38.37478241949521	40.0	38.0	41.0	34.0	41.0
17	38.351149708000655	40.0	38.0	41.0	34.0	41.0
18	38.38227948688841	40.0	38.0	41.0	34.0	41.0
19	38.41823860698023	40.0	38.0	41.0	34.0	41.0
20	38.42314678170053	40.0	38.0	41.0	34.0	41.0
21	38.38580334506376	40.0	38.0	41.0	34.0	41.0
22	38.31311884310238	40.0	38.0	41.0	34.0	41.0
23	38.30435570943858	40.0	38.0	41.0	34.0	41.0
24	38.26672216546209	40.0	38.0	41.0	34.0	41.0
25	38.25001576670324	40.0	38.0	41.0	34.0	41.0
26	38.11803427050617	40.0	38.0	41.0	33.0	41.0
27	38.06690285188128	40.0	37.0	41.0	33.0	41.0
28	38.07041725003469	40.0	37.0	41.0	33.0	41.0
29	38.012304335212725	40.0	37.0	41.0	33.0	41.0
30	37.94763089517034	40.0	37.0	41.0	33.0	41.0
31	37.91501589283687	40.0	37.0	41.0	33.0	41.0
32	37.82179210655769	40.0	37.0	41.0	33.0	41.0
33	37.75674657231871	40.0	37.0	41.0	33.0	41.0
34	37.711967558431404	40.0	37.0	41.0	33.0	41.0
35	37.71957972275829	40.0	37.0	41.0	33.0	41.0
36	37.6638743835219	40.0	37.0	41.0	33.0	41.0
37	37.60034560613514	40.0	37.0	41.0	32.0	41.0
38	37.5712276585815	40.0	37.0	41.0	32.0	41.0
39	37.47903974470554	40.0	37.0	41.0	32.0	41.0
40	37.40040961895031	40.0	37.0	41.0	32.0	41.0
41	37.36630623983048	40.0	37.0	41.0	32.0	41.0
42	37.31233208461044	40.0	37.0	41.0	31.0	41.0
43	36.66081721976261	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	7.0
11	12.0
12	10.0
13	12.0
14	5.0
15	7.0
16	10.0
17	9.0
18	18.0
19	24.0
20	57.0
21	108.0
22	232.0
23	397.0
24	732.0
25	1333.0
26	2180.0
27	3546.0
28	5140.0
29	7278.0
30	9984.0
31	13513.0
32	17627.0
33	22615.0
34	29867.0
35	40666.0
36	56650.0
37	83889.0
38	149959.0
39	188351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.76346161123094	22.920529508961792	15.362129640140765	23.953879239666502
2	13.16094020004793	22.430500372094198	42.29812313164567	22.110436296212207
3	14.483766602338518	28.92590910810913	35.59774725344029	20.992577036112056
4	11.557308812956446	19.62071618672822	37.56858515911757	31.253389841197766
5	11.856560840554483	39.280376130472625	35.93972704683341	12.923335982139477
6	26.794881497458405	40.35661129400487	18.201712894640583	14.646794313896141
7	22.994948348280168	34.492028354839114	23.006615708681778	19.506407588198936
8	23.59108739798943	36.633619656664266	22.42561269408812	17.34968025125818
9	22.978235642840026	15.747467867458786	19.658871608582132	41.615424881119054
10	13.526885382374088	29.83328287988295	34.09864910886593	22.54118262887703
11	30.096429157048977	23.4201763348091	26.20363012575522	20.279764382386702
12	19.439556766438365	27.33599475284116	29.467495364589247	23.75695311613123
13	27.82696989190348	21.3014467526898	28.123383912917344	22.748199442489373
14	20.807475940010846	23.279064340762602	26.93630882556981	28.977150893656738
15	23.717694025050136	29.155156973297515	23.868423708076335	23.258725293576017
16	24.268109635347688	28.352316444040817	23.68521461636458	23.69435930424692
17	20.626789520818356	31.327335679418777	24.99858099670791	23.047293803054956
18	22.313669101045647	27.0812048283952	26.61104173761683	23.994084332942318
19	23.03688777891298	29.16745500182894	26.617821420012362	21.17783579924572
20	22.036805792056104	25.867641679595366	27.63130510462784	24.46424742372069
21	22.591793746294826	27.036742725243123	27.086250173433733	23.28521335502832
22	20.038376155699346	31.527730477668044	25.953254878217987	22.480638488414627
23	22.97996998019702	26.688929251649196	26.609938068389656	23.721162699764132
24	23.229399225539538	28.965798867320036	26.056211450410565	21.74859045672986
25	21.685050642650825	28.188973398418284	27.58495099708631	22.541024961844577
26	22.61796647368222	29.339312067204	26.636110795777046	21.40661066333674
27	20.427813725861178	29.105334191041987	27.217429144435616	23.24942293866122
28	20.01914077773994	27.058343108689346	29.72969563956055	23.19282047401017
29	20.64318689219359	29.08452214275804	26.199057781814055	24.07323318323432
30	21.33234949105082	27.168394697342364	28.757836051512975	22.74141976009384
31	21.733612088646712	26.73386435589864	28.12921759311815	23.4033059623365
32	21.123598340081482	26.87008867193905	29.079161463654597	22.92715152432487
33	22.147645715871395	27.69452958464197	28.99701693974597	21.160807759740667
34	19.917603208839445	28.99102559251271	27.14868631828559	23.942684880362254
35	20.75355381491152	26.9095054300526	31.924263064290308	20.412677690745575
36	22.162308749889633	29.026343007782447	25.654791185782216	23.156557056545704
37	22.09435425890188	27.44904201511081	27.743091030637856	22.713512695349454
38	21.843663677299734	26.244150553095952	28.69256190007694	23.21962386952738
39	23.37918290637101	26.395510904251964	27.39590822517375	22.829397964203277
40	20.91011717813852	25.495232148938584	31.69611886832911	21.898531804593787
41	21.703497685447964	27.30398834525296	26.98518560563061	24.00732836366847
42	22.94559856712201	25.491448140159683	29.863554950114153	21.699398342604155
43	22.169403766350072	25.4938131456465	29.653384795852727	22.683398292150706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	11.5
2	14.0
3	62.0
4	110.0
5	110.0
6	149.5
7	189.0
8	209.0
9	229.0
10	362.0
11	495.0
12	495.0
13	744.0
14	993.0
15	2492.5
16	3992.0
17	4506.5
18	5021.0
19	5021.0
20	5383.0
21	5745.0
22	7235.5
23	8726.0
24	10551.0
25	12376.0
26	12376.0
27	14976.0
28	17576.0
29	22675.0
30	27774.0
31	28636.5
32	29499.0
33	29499.0
34	32090.0
35	34681.0
36	35690.5
37	36700.0
38	40211.5
39	43723.0
40	43723.0
41	46572.5
42	49422.0
43	44791.0
44	40160.0
45	41515.5
46	42871.0
47	42871.0
48	44038.5
49	45206.0
50	55651.5
51	66097.0
52	55536.5
53	44976.0
54	44976.0
55	44618.0
56	44260.0
57	36639.0
58	29018.0
59	22040.5
60	15063.0
61	15063.0
62	12866.5
63	10670.0
64	8880.0
65	7090.0
66	5912.0
67	4734.0
68	4734.0
69	3845.5
70	2957.0
71	2392.0
72	1827.0
73	1427.5
74	1028.0
75	1028.0
76	781.0
77	534.0
78	400.0
79	266.0
80	183.0
81	100.0
82	100.0
83	83.5
84	67.0
85	48.5
86	30.0
87	22.0
88	14.0
89	14.0
90	10.0
91	6.0
92	3.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	634248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.81912315586397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.11449111186398	41.55214823938777
2	9.204520703669948	8.987132596463258
3	2.1474344229107323	3.1450759668366213
4	0.867685585571629	1.6943859785035722
5	0.49582192718096996	1.2102795863212796
6	0.3049443500413743	0.8932269468212847
7	0.21773645396894822	0.7440791933281832
8	0.16790789039487913	0.655769278402313
9	0.1340738564713835	0.5890831299952292
>10	1.0455627814118498	10.158798606501742
>50	0.14439427316917222	4.927946483761347
>100	0.14050049387981992	13.39871081879843
>500	0.00908548228322161	3.077570596782046
>1k	0.0048672226517258626	4.213204918808528
>5k	6.489630202301149E-4	2.8296604678977606
>10k+	3.2448151011505745E-4	1.9229271913906352
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	12139	1.9139201069613148	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	9714	1.5315775532599236	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	8149	1.2848286474691288	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	4998	0.7880198282060014	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	2958	0.46637908199947026	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	2230	0.3515974823728258	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2001	0.3154917319408181	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1834	0.2891613375209697	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1518	0.2393385552654482	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1492	0.2352392124216395	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1364	0.2150578322675042	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1263	0.19913346198963183	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1216	0.19172311146428528	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	1216	0.19172311146428528	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1173	0.18494342906875544	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1131	0.1783214137056798	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1121	0.17674474338113796	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1082	0.1705957291154249	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	926	0.1459996720525725	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	891	0.14048132591667611	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	889	0.14016599185176776	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	844	0.13307097539132956	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	836	0.13180963913169613	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	830	0.13086363693697103	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	805	0.1269219611256165	No Hit
CCTCTTAAATTTACAGGACTTAACATTTCAAACATCCCACGTG	798	0.12581829189843718	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	779	0.12282261828180775	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	770	0.12140361498972012	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	735	0.11588526885382375	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	726	0.1144662655617361	No Hit
GGTTGATGAATACCAAGAGGAACAGGAATAACATTGCCAAACG	716	0.11288959523719427	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	702	0.11068225678283573	No Hit
CTTTGATACATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTC	666	0.10500624361448518	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	652	0.10279890516012664	No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT	650	0.10248357109521827	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	644	0.10153756890049319	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.153340649083639E-4	0.0
5	0.0	0.0	0.0	3.153340649083639E-4	0.0
6	0.0	0.0	0.0	3.153340649083639E-4	0.0
7	0.0	0.0	0.0	3.153340649083639E-4	0.0
8	0.0	0.0	0.0	3.153340649083639E-4	0.0
9	0.0	0.0	0.0	3.153340649083639E-4	0.0
10	0.0	0.0	0.0	3.153340649083639E-4	0.0
11	0.0	0.0	0.0	4.730010973625459E-4	0.0
12	0.0	0.0	0.0	4.730010973625459E-4	0.0
13	0.0	0.0	0.0	4.730010973625459E-4	0.0
14	0.0	0.0	0.0	6.306681298167278E-4	0.0
15	0.0	0.0	0.0	6.306681298167278E-4	0.0
16	0.0	0.0	0.0	6.306681298167278E-4	0.0
17	0.0	0.0	0.0	6.306681298167278E-4	0.0
18	0.0	0.0	0.0	9.460021947250918E-4	0.0
19	0.0	0.0	0.0	9.460021947250918E-4	0.0
20	1.5766703245418195E-4	0.0	0.0	9.460021947250918E-4	0.0
21	3.153340649083639E-4	0.0	0.0	0.0012613362596334556	0.0
22	3.153340649083639E-4	0.0	0.0	0.0017343373569960016	0.0
23	3.153340649083639E-4	0.0	0.0	0.0023650054868127293	0.0
24	3.153340649083639E-4	0.0	0.0	0.0031533406490836394	0.0
25	3.153340649083639E-4	0.0	0.0	0.0031533406490836394	0.0
26	3.153340649083639E-4	0.0	0.0	0.003626341746446185	0.0
27	3.153340649083639E-4	0.0	0.0	0.004257009876262913	0.0
28	3.153340649083639E-4	0.0	0.0	0.00882935381743419	0.0
29	3.153340649083639E-4	0.0	0.0	0.018920043894501835	0.0
30	3.153340649083639E-4	0.0	0.0	0.03153340649083639	0.0
31	3.153340649083639E-4	0.0	0.0	0.0707924975719277	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCGT	20	0.0018413145	37.0	11
ATCGGCG	20	0.0018413145	37.0	10
CTTATAC	1380	0.0	33.380432	1
TTATACA	1565	0.0	29.907349	2
CGGCGTA	25	0.005494508	29.6	12
TATACAC	1590	0.0	29.320753	3
CGTCTTC	395	0.0	28.56962	37
TAAGGGT	40	5.9359976E-5	27.75	4
GGTATCA	325	0.0	27.323076	1
GTATCAA	1100	0.0	26.236362	1
CCGTCTT	1365	0.0	26.02198	37
GCCGTCT	1395	0.0	25.462366	36
TCATATA	60	3.722896E-5	21.583334	2
TGCCGTC	1660	0.0	21.39759	35
ATGCCGT	1715	0.0	20.71137	34
GACCCTT	45	0.0038239465	20.555555	6
CACGAGG	75	9.25504E-6	19.733332	14
TATGCCG	1830	0.0	19.409836	33
TACACAT	2505	0.0	18.758482	5
TAATCAG	50	0.0070314887	18.5	5
>>END_MODULE