FastQCFastQC Report
Fri 10 Feb 2017
ERR1630854.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630854.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661557
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT122671.8542619910302514No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG119441.8054377778483184No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT115821.750718381031415TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC65620.9919024362224268No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC44410.671295141612892RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC32260.4876374976003579No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.31924686761684934No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20710.31304936687239343No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG16160.24427222446440747No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT13870.2096569154283002No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC12510.18909935198327582No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC10870.1643093490054523No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG10500.15871648247996772No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA10460.1581118482609964No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10240.15478636005665422No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT10230.1546352015019114No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT9740.1472284323195129No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9590.1449610539983705No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT9510.1437517855604279No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9170.1386123946991718No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC8710.13165910118100177No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8300.1254616004365459No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT8280.12515928332706025No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT8270.12500812477231743No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA8200.12395001488911764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7520.11367123316660545No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA7420.11215964761917718No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC6860.1036947685535789No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG6780.10248550011563629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG650.037.01
CTTATAC14550.032.8041231
CGTCTTC5450.030.55045937
GGTACGA250.005494677529.631
TTATACA16500.028.9272732
TTACGGG900.028.7777793
TATACAC16650.028.7777793
GGTATCA3350.028.164181
CCGTCTT16000.025.09062637
GCCGTCT16000.025.09062636
ATTACGG1002.0008883E-1124.0500012
GTATCAA9650.023.1968921
CGGTTTG400.001930325923.12523
TGCCGTC19050.020.97637735
CCTACGA450.003824116220.5555572
CTCGAAA450.003824116220.55555728
ATGCCGT20050.020.02244434
CTTAATA609.2311675E-418.52
GTTTAGG701.2182051E-418.51
TATGCCG21900.018.533
Produced by FastQC (version 0.11.5)