##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630854.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661557
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259761441568905	31.0	31.0	34.0	28.0	34.0
2	31.267612616902248	31.0	31.0	34.0	28.0	34.0
3	30.712139694689952	31.0	31.0	34.0	26.0	34.0
4	34.908157573723805	37.0	35.0	37.0	32.0	37.0
5	35.0817299189639	37.0	35.0	37.0	32.0	37.0
6	35.27958437443788	37.0	35.0	37.0	32.0	37.0
7	35.27367558653298	37.0	35.0	37.0	32.0	37.0
8	35.3134574949702	37.0	35.0	37.0	32.0	37.0
9	37.05135309580278	39.0	37.0	39.0	33.0	39.0
10	36.87444921601615	39.0	37.0	39.0	33.0	39.0
11	36.9334630273733	39.0	37.0	39.0	33.0	39.0
12	36.86208444623819	39.0	37.0	39.0	33.0	39.0
13	36.94201255522956	39.0	37.0	39.0	33.0	39.0
14	38.000288712839556	40.0	37.0	41.0	33.0	41.0
15	38.02137684281173	40.0	37.0	41.0	33.0	41.0
16	38.01653523430332	40.0	37.0	41.0	33.0	41.0
17	37.97539743363006	40.0	37.0	41.0	33.0	41.0
18	38.05218295626832	40.0	37.0	41.0	33.0	41.0
19	38.051799013539274	40.0	37.0	41.0	33.0	41.0
20	38.007755945443854	40.0	37.0	41.0	33.0	41.0
21	37.98744628202861	40.0	37.0	41.0	33.0	41.0
22	37.90977194708846	40.0	37.0	41.0	33.0	41.0
23	37.909729622693135	40.0	37.0	41.0	33.0	41.0
24	37.868513219571405	40.0	37.0	41.0	33.0	41.0
25	37.791903040856646	39.0	37.0	41.0	33.0	41.0
26	37.667331764307534	39.0	37.0	41.0	32.0	41.0
27	37.577342541912486	39.0	37.0	41.0	32.0	41.0
28	37.58099453259508	39.0	37.0	41.0	32.0	41.0
29	37.56486289163292	39.0	37.0	41.0	32.0	41.0
30	37.419622194308275	39.0	37.0	41.0	32.0	41.0
31	37.41739714038246	39.0	36.0	40.0	32.0	41.0
32	37.331407573345906	39.0	36.0	40.0	32.0	41.0
33	37.27423185001444	39.0	36.0	40.0	31.0	41.0
34	37.23080550882237	39.0	36.0	40.0	31.0	41.0
35	37.22270643345925	39.0	36.0	40.0	31.0	41.0
36	37.16842993120774	39.0	36.0	40.0	31.0	41.0
37	37.125166841254796	39.0	36.0	40.0	31.0	41.0
38	37.081760150674846	39.0	36.0	40.0	31.0	41.0
39	36.9866904892549	39.0	36.0	40.0	31.0	41.0
40	36.89130944121217	39.0	36.0	40.0	31.0	41.0
41	36.855877875980454	39.0	36.0	40.0	30.0	41.0
42	36.8174110469695	39.0	36.0	40.0	30.0	41.0
43	36.089339240609654	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	20.0
11	14.0
12	14.0
13	10.0
14	9.0
15	11.0
16	7.0
17	12.0
18	28.0
19	48.0
20	79.0
21	159.0
22	318.0
23	583.0
24	1146.0
25	1920.0
26	2950.0
27	4486.0
28	6604.0
29	9238.0
30	12772.0
31	16684.0
32	21120.0
33	27753.0
34	36588.0
35	48186.0
36	67146.0
37	98737.0
38	166563.0
39	138336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.04291542527704	23.03716837702571	15.902333434609565	24.017582763087685
2	13.53609741866536	22.562228198023753	41.05043102861885	22.851243354692038
3	14.76728384704568	28.528909829387338	35.71876648572987	20.985039837837103
4	11.807145869516912	19.495825756510776	36.623601594420435	32.07342677955188
5	12.159798777731927	39.09685786712256	35.1809443479549	13.562399007190612
6	27.160622591855276	39.81924460023853	17.88205702607636	15.138075781829835
7	23.07072557617862	34.812570950046634	22.372221894712023	19.744481579062725
8	24.364642804777215	35.6108392776435	22.473498126389714	17.551019791189574
9	22.908834764049054	15.428753682600291	19.232205237039288	42.43020631631137
10	13.803799219114907	29.161810698095554	33.57352427681968	23.460865805969856
11	30.103830811252845	23.066644295200565	25.65281600829558	21.176708885251006
12	19.283901462761335	27.369221397400374	28.789507177763973	24.55736996207432
13	28.313508888878815	20.750441760876235	27.592784899865013	23.343264450379937
14	21.225230781323454	23.44816848737146	26.30143736669705	29.025163364608037
15	23.964223793263468	29.102103068972134	23.20072193325745	23.732951204506943
16	24.660762413518412	28.248813027448882	22.991367334938637	24.09905722409407
17	20.58991137573935	31.557371473659867	24.619647286628364	23.233069863972418
18	23.312730422321888	26.0623045330939	25.84901981235177	24.775945232232445
19	24.959754034799722	27.703432961936763	26.577301729102707	20.759511274160804
20	22.260818039866557	26.23734613948609	27.9492167719486	23.55261904869875
21	24.348166522310247	27.83781291710314	25.132074787206545	22.681945773380072
22	21.688531751610217	31.78788826964268	25.784323950921838	20.739256027825267
23	22.805139995495473	28.321369133725437	25.83072962722789	23.042761243551197
24	27.091996608002034	27.740013332184528	25.42532238340763	19.742667676405812
25	23.351275853781306	28.43020329314027	26.502780561614493	21.715740291463923
26	25.03291477529525	29.163322283642984	25.247711081584807	20.556051859476963
27	22.29528219034792	29.200809605219202	28.037191050808925	20.46671715362395
28	19.97620764348348	29.30027193423998	27.75059443101653	22.972925991260013
29	25.702396014251228	26.676915216678232	25.907064697373016	21.713624071697527
30	20.937122575983626	28.732973878290153	29.66955228347671	20.66035126224951
31	23.81835578793664	28.645453075094057	25.532644957275036	22.00354617969427
32	25.628328322427247	26.748715530181073	27.22350455062829	20.39945159676339
33	20.51962264778394	29.703714116848584	28.939758781178345	20.83690445418913
34	22.342745976537167	29.361339990356083	26.329250540769728	21.966663492337016
35	23.05364465949268	26.24807839687283	30.450286218723406	20.24799072491108
36	22.520659595469475	28.41705249887765	25.104261613133865	23.958026292519012
37	22.4095580577335	26.693391499145196	27.873335177467702	23.023715265653603
38	22.18236674995503	25.232292909001035	28.47116726147558	24.114173079568353
39	24.388677014981326	25.880763108847766	26.85135218885145	22.87920768731946
40	20.74741858978138	24.877070305355396	32.23592222590042	22.139588878962808
41	21.91058366852743	26.785295900428835	26.72347205153902	24.580648379504712
42	22.7593389534084	24.89853482012888	30.3397893152064	22.00233691125632
43	22.33594384157374	24.846989752961573	29.910952495401	22.906113910063684
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	14.5
2	23.0
3	71.0
4	119.0
5	119.0
6	170.5
7	222.0
8	219.0
9	216.0
10	369.5
11	523.0
12	523.0
13	735.5
14	948.0
15	2553.0
16	4158.0
17	4522.0
18	4886.0
19	4886.0
20	5330.0
21	5774.0
22	7232.5
23	8691.0
24	10718.0
25	12745.0
26	12745.0
27	14789.5
28	16834.0
29	21354.0
30	25874.0
31	27465.0
32	29056.0
33	29056.0
34	31307.5
35	33559.0
36	35338.0
37	37117.0
38	40168.5
39	43220.0
40	43220.0
41	45946.5
42	48673.0
43	45054.0
44	41435.0
45	42666.0
46	43897.0
47	43897.0
48	45754.5
49	47612.0
50	59437.0
51	71262.0
52	59578.5
53	47895.0
54	47895.0
55	49885.0
56	51875.0
57	43083.5
58	34292.0
59	25490.0
60	16688.0
61	16688.0
62	14378.0
63	12068.0
64	10161.5
65	8255.0
66	6895.5
67	5536.0
68	5536.0
69	4475.5
70	3415.0
71	2805.0
72	2195.0
73	1734.5
74	1274.0
75	1274.0
76	928.5
77	583.0
78	475.5
79	368.0
80	258.0
81	148.0
82	148.0
83	102.5
84	57.0
85	46.0
86	35.0
87	25.5
88	16.0
89	16.0
90	10.5
91	5.0
92	3.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	661557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.845021644492704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38335325153841	37.76866612962667
2	10.325992684983744	9.46790716287906
3	2.8103576431904767	3.8652272094249875
4	1.1899740551741553	2.1821754526337562
5	0.6674992127103182	1.530075792719319
6	0.41573629314546207	1.1435663614592924
7	0.2867296418124467	0.9201588644506481
8	0.20487352975484843	0.7513945124795718
9	0.17890359226394684	0.7381655153656326
>10	1.2152642298552843	10.96707398165795
>50	0.1616516940078603	5.198278200565863
>100	0.1457515259081707	13.015589483892454
>500	0.008612590167300992	2.7797044537508158
>1k	0.003975041615677381	3.23985002274475
>5k	3.312534679731151E-4	0.9965264764812529
>10k+	9.937604039193452E-4	5.43564037986795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	12267	1.8542619910302514	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	11944	1.8054377778483184	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	11582	1.750718381031415	TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	6562	0.9919024362224268	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	4441	0.671295141612892	RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	3226	0.4876374976003579	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2112	0.31924686761684934	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2071	0.31304936687239343	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	1616	0.24427222446440747	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1387	0.2096569154283002	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1251	0.18909935198327582	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1087	0.1643093490054523	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1050	0.15871648247996772	No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	1046	0.1581118482609964	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1024	0.15478636005665422	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1023	0.1546352015019114	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	974	0.1472284323195129	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	959	0.1449610539983705	No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	951	0.1437517855604279	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	917	0.1386123946991718	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	871	0.13165910118100177	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	830	0.1254616004365459	No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT	828	0.12515928332706025	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	827	0.12500812477231743	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	820	0.12395001488911764	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	752	0.11367123316660545	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	742	0.11215964761917718	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	686	0.1036947685535789	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	678	0.10248550011563629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.511585547428264E-4	0.0
12	0.0	0.0	0.0	1.511585547428264E-4	0.0
13	0.0	1.511585547428264E-4	0.0	1.511585547428264E-4	0.0
14	0.0	1.511585547428264E-4	0.0	1.511585547428264E-4	0.0
15	0.0	3.023171094856528E-4	0.0	1.511585547428264E-4	0.0
16	0.0	3.023171094856528E-4	0.0	3.023171094856528E-4	0.0
17	0.0	3.023171094856528E-4	0.0	3.023171094856528E-4	0.0
18	0.0	3.023171094856528E-4	0.0	3.023171094856528E-4	0.0
19	0.0	3.023171094856528E-4	0.0	6.046342189713056E-4	0.0
20	0.0	3.023171094856528E-4	0.0	6.046342189713056E-4	0.0
21	0.0	3.023171094856528E-4	0.0	0.001209268437942611	0.0
22	0.0	3.023171094856528E-4	0.0	0.001511585547428264	0.0
23	0.0	3.023171094856528E-4	0.0	0.0021162197663995696	0.0
24	0.0	3.023171094856528E-4	0.0	0.003023171094856528	0.0
25	0.0	3.023171094856528E-4	0.0	0.0033254882043421805	0.0
26	0.0	3.023171094856528E-4	0.0	0.0036278053138278335	0.0
27	0.0	3.023171094856528E-4	0.0	0.004081280978056313	0.0
28	0.0	3.023171094856528E-4	0.0	0.008616037620341105	0.0
29	0.0	3.023171094856528E-4	0.0	0.017534392350167863	0.0
30	0.0	3.023171094856528E-4	0.0	0.029475918174851148	0.0
31	0.0	3.023171094856528E-4	0.0	0.06333543443724426	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	65	0.0	37.0	1
CTTATAC	1455	0.0	32.804123	1
CGTCTTC	545	0.0	30.550459	37
GGTACGA	25	0.0054946775	29.6	31
TTATACA	1650	0.0	28.927273	2
TTACGGG	90	0.0	28.777779	3
TATACAC	1665	0.0	28.777779	3
GGTATCA	335	0.0	28.16418	1
CCGTCTT	1600	0.0	25.090626	37
GCCGTCT	1600	0.0	25.090626	36
ATTACGG	100	2.0008883E-11	24.050001	2
GTATCAA	965	0.0	23.196892	1
CGGTTTG	40	0.0019303259	23.125	23
TGCCGTC	1905	0.0	20.976377	35
CCTACGA	45	0.0038241162	20.555557	2
CTCGAAA	45	0.0038241162	20.555557	28
ATGCCGT	2005	0.0	20.022444	34
CTTAATA	60	9.2311675E-4	18.5	2
GTTTAGG	70	1.2182051E-4	18.5	1
TATGCCG	2190	0.0	18.5	33
>>END_MODULE