FastQCFastQC Report
Fri 10 Feb 2017
ERR1630853.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630853.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222768
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG83453.746049701932055No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT81723.6683904330963157RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT76563.4367593191122605No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC50642.273216979099332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42371.9019787402140342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39411.7691050779286073No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC27501.2344681462328522RNA PCR Primer, Index 41 (95% over 23bp)
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC27431.231325863678805No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA23191.040993320405085No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14180.6365366659484306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14050.6307009983480572No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT13030.5849134525605114No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13000.5835667600373483No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG12990.5831178625296273No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC9890.4439596351361057No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9880.44351073762838467No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9560.42914601738131153No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9200.4129857071033542No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8630.38739854916325506No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7800.350140056022409No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7050.31647274294333116No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA7010.314677152912447No Hit
ACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT6740.30255692020397906No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6540.2935789700495583No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6340.28460101989513753No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT6310.2832543273719744No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5950.2670940170940171No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5920.265747324570854No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5830.2617072470013646No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5810.26080945198592254No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5580.2504848093083387No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5500.2468936292465704No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG5380.24150685915391798No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5130.23028442146089206No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5090.22848883143000792No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5050.22669324139912378No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4710.2114307261366085No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4710.2114307261366085No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4680.21008403361344538No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4490.2015549809667457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.19257703081232494No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4280.19212813330460388No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4270.19167923579688284No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4100.1840479781656252No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3860.17327443798032033No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3840.17237664296487826No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3770.169234360410831No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3750.16833656539538894No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3740.1678876678876679No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3640.16339869281045752No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3620.16250089779501545No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.1620520002872944No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3510.15756302521008403No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3510.15756302521008403No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3480.15621633268692092No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3430.15397184514831574No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3380.15172735760971057No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3270.14678948502477915No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3240.14544279250161604No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3220.14454499748617397No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3180.1427494074552898No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3100.13915822739352152No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3060.13736263736263737No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3050.1369137398549163No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3040.13646484234719528No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3020.1355670473317532No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3000.13466925231631113No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2980.13377145730086906No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2960.132873662285427No Hit
ATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCT2950.13242476477770596No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2790.12524240465416936No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2760.12389571213100624No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2740.12299791711556417No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2740.12299791711556417No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2660.11940673705379588No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2630.11806004453063276No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2620.11761114702291173No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2550.11446886446886448No Hit
TCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTCT2520.11312217194570137TruSeq Adapter, Index 3 (95% over 23bp)
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2510.11267327443798031No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2500.11222437693025927No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2460.11042878689937513No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2420.10863319686849099No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2410.10818429936076995No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA2400.10773540185304892No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2390.10728650434532788No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT2380.10683760683760685No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2360.10593981182216476No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2350.10549091431444373No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2350.10549091431444373No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2340.10504201680672269No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2290.10279752926811751No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG2260.1014508367449544No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2230.10010414422179127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCGT200.001838752237.016
ACGCATC200.001838752237.01
TTACACA308.281913E-637.03
GGATCAA308.281913E-637.02
ATTACTT403.8096914E-837.02
TAGCAAC251.2304328E-437.01
AGGTGGC200.001838752237.010
ATTACGG551.2732926E-1137.02
ACTCAGA501.7826096E-1037.01
TAGTATT200.001838752237.02
TATCTAT200.001838752237.017
TCAGAGT501.7826096E-1037.03
GATCAAC308.281913E-637.03
GTGGCTA200.001838752237.012
GATTACG551.2732926E-1137.01
ATACTAT200.001838752237.01
AGCAACG251.2304328E-437.02
TTTACAC251.2304328E-437.02
TATAGGT200.001838752237.07
TACTATA200.001838752237.02
Produced by FastQC (version 0.11.5)