FastQCFastQC Report
Fri 10 Feb 2017
ERR1630852.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630852.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173271
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG123577.131603095728655No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT111326.42461808381091RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC67123.873700734687282No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT62383.6001408198717617No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC44642.5763110964904685RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC35652.0574706673361383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29121.680604371187331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25051.4457122080440465No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG15570.8985923784130063No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11370.6561975171840643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10930.6308037698172227No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA10890.628495247329328No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT9620.5551996583386718No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT8180.4720928487744631No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8090.4668986731767001No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7920.4570874526031477No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7920.4570874526031477No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5950.3433927200743344No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5930.3422384588303871No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5460.3151133195976245No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG5060.2920280947186777No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4980.28741104974288834No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG4840.2793312210352569No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4320.249320428692626No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT4160.24008633874104726No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3980.22969798754552115No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3780.21815537510604774No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.20257284831275862No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3410.19680154209302192No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3390.19564728084907457No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG3340.19276162773920621No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3290.18987597462933786No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3240.1869903215194695No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3090.17833336218986443No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3020.17429344783604872No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2930.1690992722382857No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2800.16159657415262796No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2770.15986518228670696No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2730.15755665979881225No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2700.15582526793289125No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2680.1546710066889439No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA2610.1506310923351282No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2540.14659117798131252No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2520.14543691673736517No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2500.14428265549341782No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2450.14139700238354946No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2450.14139700238354946No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2430.14024274113960214No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2270.13100865118802338No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2240.12927725932210238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.12812299807815503No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC2210.12754586745618138No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2170.12523734496828667No Hit
CTGATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT2120.12235169185841831No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC2120.12235169185841831No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2110.12177456123644463No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2100.12119743061447098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2080.12004316937052363No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2080.12004316937052363No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2040.11773464688262895No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1930.11138621004091857No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1920.1108090794189449No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1920.1108090794189449No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1830.10561490382118184No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1830.10561490382118184No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1820.10503777319920819No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1820.10503777319920819No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1810.10446064257723452No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1800.10388351195526084No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1790.10330638133328716No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1770.10215212008933981No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT1750.10099785884539249No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC1740.10042072822341881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCAAC200.001837624937.01
TTAGCAC200.001837624937.030
CGCCGAG251.2293027E-437.02
CGTCTTC4800.034.687537
CTTATAC6800.034.5514681
CTACACA551.8366336E-830.2727284
TTATACA7800.029.8846172
TGATGGA250.005483627329.626
GATGGAG250.005483627329.627
TGGAGGG250.005483627329.629
ATGGAGG250.005483627329.628
TCGCCGA250.005483627329.61
TTTACAC502.698289E-729.62
GCCGAGC250.005483627329.63
GGTATCA3100.028.6451631
CAGACAC358.8389E-426.4285718
TATACAC9100.026.2252773
CCGTCTT15700.025.80573337
GCCGTCT16150.025.08668736
TTACACA601.3264853E-624.6666663
Produced by FastQC (version 0.11.5)