##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630852.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	173271
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.384801842200947	31.0	30.0	33.0	27.0	34.0
2	30.3879472040907	31.0	30.0	33.0	27.0	34.0
3	29.785440148668847	31.0	30.0	34.0	25.0	34.0
4	33.979494549001274	35.0	33.0	37.0	28.0	37.0
5	33.963617685590776	35.0	33.0	37.0	30.0	37.0
6	34.24941854089836	35.0	35.0	37.0	30.0	37.0
7	34.25715209123281	35.0	35.0	37.0	30.0	37.0
8	34.326044173577806	35.0	35.0	37.0	30.0	37.0
9	35.94988774809403	37.0	35.0	39.0	31.0	39.0
10	35.73689769205464	37.0	35.0	39.0	30.0	39.0
11	35.78387035337708	37.0	35.0	39.0	30.0	39.0
12	35.62102140577477	37.0	35.0	39.0	30.0	39.0
13	35.81410622666228	37.0	35.0	39.0	30.0	39.0
14	36.3677418610154	38.0	35.0	40.0	30.0	41.0
15	36.40128469276451	38.0	35.0	40.0	30.0	41.0
16	36.42706511764808	38.0	35.0	40.0	31.0	41.0
17	36.39940324693688	38.0	35.0	40.0	30.0	41.0
18	36.587132295652474	38.0	36.0	40.0	31.0	41.0
19	36.42390244183966	38.0	35.0	40.0	30.0	41.0
20	36.489216314328424	38.0	35.0	40.0	30.0	41.0
21	36.411453734323686	38.0	35.0	40.0	30.0	41.0
22	36.0964385269318	38.0	34.0	40.0	30.0	41.0
23	36.24706961926693	38.0	35.0	40.0	30.0	41.0
24	35.94974923674475	38.0	34.0	40.0	29.0	41.0
25	35.912558939464766	38.0	34.0	40.0	29.0	41.0
26	35.63030166617611	38.0	34.0	40.0	28.0	41.0
27	35.3537349008201	38.0	34.0	40.0	27.0	41.0
28	35.50104749207888	38.0	34.0	40.0	27.0	41.0
29	35.32285841254451	38.0	33.0	40.0	27.0	41.0
30	35.15023287220597	37.0	33.0	40.0	27.0	41.0
31	35.06442509133092	37.0	33.0	40.0	27.0	41.0
32	34.65309255443785	37.0	33.0	40.0	26.0	40.0
33	34.54112921377495	37.0	33.0	39.0	26.0	40.0
34	34.36030264729816	37.0	33.0	39.0	25.0	40.0
35	34.321028908472854	37.0	33.0	39.0	24.0	40.0
36	34.27309243901172	37.0	33.0	39.0	24.0	41.0
37	34.211050897149555	37.0	33.0	40.0	24.0	41.0
38	34.04494693283931	37.0	33.0	39.0	23.0	41.0
39	33.72177686975893	37.0	32.0	39.0	23.0	40.0
40	33.20659544874791	36.0	31.0	39.0	22.0	40.0
41	33.17678088081676	36.0	31.0	39.0	21.0	40.0
42	32.941530896687844	36.0	31.0	39.0	20.0	40.0
43	31.84873983528692	35.0	29.0	38.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	19.0
11	13.0
12	8.0
13	9.0
14	13.0
15	3.0
16	15.0
17	17.0
18	33.0
19	82.0
20	130.0
21	226.0
22	384.0
23	642.0
24	1090.0
25	1591.0
26	2242.0
27	3244.0
28	4383.0
29	5577.0
30	6985.0
31	8609.0
32	10842.0
33	13187.0
34	16320.0
35	19564.0
36	23163.0
37	23835.0
38	21281.0
39	9753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.65965452960969	25.47454565391785	19.459113181086277	23.406686635386187
2	12.601647130795113	22.517905477546734	40.308534030507126	24.57191336115103
3	15.081000282794005	32.656359113758214	34.57762695430857	17.685013649139208
4	9.414154705634527	18.741739817973002	26.844076619861372	45.0000288565311
5	13.329985975725887	39.132918953546756	27.11763653467689	20.419458536050463
6	25.865263084994027	34.694784470569225	15.879171932983594	23.560780511453157
7	20.933104789607032	36.59181282499668	15.135827691881504	27.339254693514782
8	29.011779235994485	25.393170236219564	26.044173577805864	19.55087694998009
9	19.451610483000618	11.193448413179356	14.212995827345603	55.141945276474424
10	15.897062982264776	24.223903596100904	27.734012038944773	32.14502138268954
11	30.136606818221168	19.43314230309746	19.55433973371193	30.875911144969443
12	18.20269981704959	29.309578636932898	20.542387358530856	31.94533418748665
13	38.06465017227349	15.397267863635577	20.988509329316503	25.54957263477443
14	23.748925094216574	26.79155773326177	22.446341280421997	27.01317589209966
15	29.94211379861604	25.75272261370916	18.146718146718147	26.15844544095665
16	27.89214582936556	22.046389759394245	17.407413819969875	32.65405059127032
17	18.096507782606437	33.49492990748596	18.284652365369855	30.123909944537747
18	24.222172204234983	17.959150694576703	21.45656226373715	36.36211483745116
19	24.602501284115633	34.86676939591738	20.924447830277426	19.60628148968956
20	22.35400038090621	25.86757160748192	22.069474984273192	29.708953027338676
21	36.619515094851415	22.216643292876476	21.708768345539646	19.45507326673246
22	19.7303645734139	41.73866371175789	21.590456568035044	16.94051514679317
23	36.88441805033733	18.069382643373675	22.12430239336069	22.921896912928304
24	28.034119962371086	31.114266091844566	21.780332542664382	19.07128140311997
25	20.78016517478401	29.049869857044747	21.626815797219383	28.54314917095186
26	27.714389597797666	35.17899706240513	21.31343386948768	15.793179470309514
27	18.54320688401406	39.26161908224689	24.789491605635103	17.40568242810395
28	27.487577263362017	21.103358323089264	28.574891355160414	22.834173058388306
29	21.344021792452285	31.76873221716271	22.920165521062383	23.96708046932262
30	24.04557023391104	23.02347190239567	31.16793923968812	21.76301862400517
31	34.42872725383936	20.42176705853836	20.878854511141505	24.270651176480772
32	22.85379549953541	24.276999613322484	27.45872073226333	25.410484154878777
33	21.380381021636627	26.28368278592494	33.15096005678965	19.184976135648782
34	20.584517893934933	30.860905748798125	25.416832591720485	23.137743765546457
35	18.36775917493406	19.16535369450168	41.51646842229802	20.95041870826624
36	23.595408348771578	23.642155929151446	22.82724749092462	29.935188231152353
37	21.64990102209833	21.30246838767018	31.313953287047458	25.733677303184027
38	20.89501416855677	17.006308037698172	29.081612041253297	33.01706575249176
39	31.597901553058506	18.93161579260234	25.845640643846924	23.624842010492234
40	17.71329304961592	17.855267182621443	42.15823767393274	22.273202093829898
41	24.99437297643576	22.192980937375555	23.538272417196186	29.274373668992503
42	20.960807059461768	17.17598444055843	39.62463424346833	22.238574256511477
43	25.71924903763469	17.33469536160119	31.29202232341246	25.65403327735166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	3.5
2	6.0
3	30.0
4	54.0
5	54.0
6	110.0
7	166.0
8	179.0
9	192.0
10	312.0
11	432.0
12	432.0
13	721.0
14	1010.0
15	2648.5
16	4287.0
17	4245.0
18	4203.0
19	4203.0
20	3486.5
21	2770.0
22	2168.0
23	1566.0
24	1414.5
25	1263.0
26	1263.0
27	1215.0
28	1167.0
29	1091.5
30	1016.0
31	1050.5
32	1085.0
33	1085.0
34	1284.5
35	1484.0
36	1436.0
37	1388.0
38	1541.0
39	1694.0
40	1694.0
41	2253.5
42	2813.0
43	3949.5
44	5086.0
45	6993.5
46	8901.0
47	8901.0
48	12659.0
49	16417.0
50	24794.0
51	33171.0
52	24574.5
53	15978.0
54	15978.0
55	24189.0
56	32400.0
57	26352.0
58	20304.0
59	13066.0
60	5828.0
61	5828.0
62	4829.5
63	3831.0
64	3063.0
65	2295.0
66	1793.0
67	1291.0
68	1291.0
69	1026.0
70	761.0
71	522.0
72	283.0
73	182.0
74	81.0
75	81.0
76	52.0
77	23.0
78	18.0
79	13.0
80	10.5
81	8.0
82	8.0
83	4.5
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	173271.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.214334770388582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17624899327116	16.92204696689002
2	9.724351147021382	4.320399836094904
3	3.8788288171260814	2.584968055820074
4	2.109584058611104	1.8745202601704845
5	1.402925359174872	1.5582526793289124
6	0.9716557117248188	1.2950811157089184
7	0.7456288483022006	1.1594554195451057
8	0.5299940245771739	0.9418771750610316
9	0.39749551843288045	0.7947088664577453
>10	3.304668623834143	14.880158826347166
>50	0.43906367722324696	6.836112217278137
>100	0.26499701228858696	11.527029912680137
>500	0.0233820893195812	3.7011386787171543
>1k	0.02078407939518329	10.574187255801606
>5k	0.005196019848795822	7.473841554559043
>10k+	0.005196019848795822	13.556221179539566
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	12357	7.131603095728655	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	11132	6.42461808381091	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	6712	3.873700734687282	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	6238	3.6001408198717617	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	4464	2.5763110964904685	RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	3565	2.0574706673361383	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	1.680604371187331	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2505	1.4457122080440465	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	1557	0.8985923784130063	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1137	0.6561975171840643	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1093	0.6308037698172227	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1089	0.628495247329328	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	962	0.5551996583386718	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	818	0.4720928487744631	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	809	0.4668986731767001	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	792	0.4570874526031477	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	792	0.4570874526031477	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	595	0.3433927200743344	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	593	0.3422384588303871	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	546	0.3151133195976245	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	506	0.2920280947186777	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	498	0.28741104974288834	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	484	0.2793312210352569	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	432	0.249320428692626	No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT	416	0.24008633874104726	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	398	0.22969798754552115	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	378	0.21815537510604774	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	351	0.20257284831275862	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	341	0.19680154209302192	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	339	0.19564728084907457	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	334	0.19276162773920621	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	329	0.18987597462933786	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	324	0.1869903215194695	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	309	0.17833336218986443	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	302	0.17429344783604872	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	293	0.1690992722382857	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	280	0.16159657415262796	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	277	0.15986518228670696	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	273	0.15755665979881225	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	270	0.15582526793289125	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	268	0.1546710066889439	No Hit
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	261	0.1506310923351282	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	254	0.14659117798131252	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	252	0.14543691673736517	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	250	0.14428265549341782	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	245	0.14139700238354946	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	245	0.14139700238354946	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	243	0.14024274113960214	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	227	0.13100865118802338	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	224	0.12927725932210238	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.12812299807815503	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	221	0.12754586745618138	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	217	0.12523734496828667	No Hit
CTGATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	212	0.12235169185841831	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	212	0.12235169185841831	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	211	0.12177456123644463	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	210	0.12119743061447098	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	208	0.12004316937052363	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	208	0.12004316937052363	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	204	0.11773464688262895	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	193	0.11138621004091857	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	192	0.1108090794189449	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	192	0.1108090794189449	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	183	0.10561490382118184	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	183	0.10561490382118184	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	182	0.10503777319920819	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	182	0.10503777319920819	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	181	0.10446064257723452	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	180	0.10388351195526084	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	179	0.10330638133328716	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	177	0.10215212008933981	No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCT	175	0.10099785884539249	No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC	174	0.10042072822341881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	5.771306219736713E-4	0.0
12	0.0	0.0	0.0	5.771306219736713E-4	0.0
13	0.0	0.0	0.0	5.771306219736713E-4	0.0
14	0.0	0.0	0.0	5.771306219736713E-4	0.0
15	0.0	0.0	0.0	5.771306219736713E-4	0.0
16	0.0	0.0	0.0	0.0011542612439473425	0.0
17	0.0	0.0	0.0	0.0011542612439473425	0.0
18	0.0	0.0	0.0	0.0011542612439473425	0.0
19	0.0	0.0	0.0	0.0017313918659210138	0.0
20	0.0	0.0	0.0	0.0017313918659210138	0.0
21	0.0	0.0	0.0	0.0034627837318420276	0.0
22	0.0	0.0	0.0	0.004039914353815699	0.0
23	0.0	0.0	0.0	0.004039914353815699	0.0
24	0.0	0.0	0.0	0.004039914353815699	0.0
25	0.0	0.0	0.0	0.005194175597763042	0.0
26	0.0	0.0	0.0	0.006925567463684055	0.0
27	0.0	0.0	0.0	0.008079828707631399	0.0
28	0.0	0.0	0.0	0.01846817990315748	0.0
29	0.0	0.0	0.0	0.042130535404078004	0.0
30	0.0	0.0	0.0	0.06463862966105119	0.0
31	0.0	0.0	0.0	0.10965481817499755	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCAAC	20	0.0018376249	37.0	1
TTAGCAC	20	0.0018376249	37.0	30
CGCCGAG	25	1.2293027E-4	37.0	2
CGTCTTC	480	0.0	34.6875	37
CTTATAC	680	0.0	34.551468	1
CTACACA	55	1.8366336E-8	30.272728	4
TTATACA	780	0.0	29.884617	2
TGATGGA	25	0.0054836273	29.6	26
GATGGAG	25	0.0054836273	29.6	27
TGGAGGG	25	0.0054836273	29.6	29
ATGGAGG	25	0.0054836273	29.6	28
TCGCCGA	25	0.0054836273	29.6	1
TTTACAC	50	2.698289E-7	29.6	2
GCCGAGC	25	0.0054836273	29.6	3
GGTATCA	310	0.0	28.645163	1
CAGACAC	35	8.8389E-4	26.42857	18
TATACAC	910	0.0	26.225277	3
CCGTCTT	1570	0.0	25.805733	37
GCCGTCT	1615	0.0	25.086687	36
TTACACA	60	1.3264853E-6	24.666666	3
>>END_MODULE