FastQCFastQC Report
Fri 10 Feb 2017
ERR1630851.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630851.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406006
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG74981.8467707373782654No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT70211.7292847888947454No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT66681.6423402609813649TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC43871.0805259035580754No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC22000.5418639133411822Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC21040.5182189425772034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT19230.4736383206159515No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.46649556903099954No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG11360.2797988207070831No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6730.16576109712664347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6420.15812574198410861No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA5880.1448254459293705No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT5620.1384215996807929No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT5590.13768269434441854No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC4460.1098505933409851No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCTA200.001840534437.014
CTTATAC9650.033.1658061
CGTCTTC3400.031.01470637
TTATACA10250.030.6829282
TATACAC10400.030.5961533
GGTATCA2350.029.127661
CCGTATT358.8594755E-426.4285721
CCGTCTT10200.024.8480437
GCCGTCT10250.024.72683136
CCGCGTG451.321154E-424.66666637
GTATCAA7850.024.5095541
GCCGCGT551.8987319E-523.54545436
CCGCCGT400.001929048123.12524
TTAGGAC753.7284553E-722.23
TTTCGCC502.697933E-422.23
ACGGAGT502.697933E-422.223
ATGCCGC603.7186506E-521.58333234
TGCCGCG603.7186506E-521.58333235
TTACGGG450.003821611420.5555553
TAGGACC450.003821611420.5555554
Produced by FastQC (version 0.11.5)