##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630851.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	406006
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.388186381482047	31.0	31.0	34.0	28.0	34.0
2	31.400521174563924	31.0	31.0	34.0	28.0	34.0
3	30.867792594198114	31.0	31.0	34.0	27.0	34.0
4	35.02658581400275	37.0	35.0	37.0	32.0	37.0
5	35.204129988226775	37.0	35.0	37.0	32.0	37.0
6	35.39560006502367	37.0	35.0	37.0	33.0	37.0
7	35.39636606355571	37.0	35.0	37.0	33.0	37.0
8	35.42896164096097	37.0	35.0	37.0	33.0	37.0
9	37.183076112175684	39.0	37.0	39.0	34.0	39.0
10	37.01555395733068	39.0	37.0	39.0	33.0	39.0
11	37.06723053353891	39.0	37.0	39.0	33.0	39.0
12	36.978394407964416	39.0	37.0	39.0	33.0	39.0
13	37.07086594779388	39.0	37.0	39.0	33.0	39.0
14	38.159239026024245	40.0	37.0	41.0	33.0	41.0
15	38.16470446249563	40.0	37.0	41.0	33.0	41.0
16	38.1729629611385	40.0	38.0	41.0	33.0	41.0
17	38.1587587375556	40.0	38.0	41.0	33.0	41.0
18	38.199130062117305	40.0	38.0	41.0	33.0	41.0
19	38.19637887124821	40.0	38.0	41.0	33.0	41.0
20	38.21137372354103	40.0	38.0	41.0	34.0	41.0
21	38.17262552770156	40.0	38.0	41.0	33.0	41.0
22	38.08716127347872	40.0	37.0	41.0	33.0	41.0
23	38.07883627335556	40.0	37.0	41.0	33.0	41.0
24	38.03704378753023	40.0	37.0	41.0	33.0	41.0
25	37.99383506647685	40.0	37.0	41.0	33.0	41.0
26	37.875987054378506	40.0	37.0	41.0	33.0	41.0
27	37.77509445673217	40.0	37.0	41.0	33.0	41.0
28	37.77663876888519	40.0	37.0	41.0	33.0	41.0
29	37.694622739565425	40.0	37.0	41.0	32.0	41.0
30	37.590885356374045	39.0	37.0	41.0	32.0	41.0
31	37.582474150628315	39.0	37.0	41.0	32.0	41.0
32	37.41988049437693	39.0	36.0	41.0	32.0	41.0
33	37.40072067900474	39.0	36.0	41.0	32.0	41.0
34	37.30959887292306	39.0	36.0	41.0	31.0	41.0
35	37.26782609124003	39.0	36.0	41.0	31.0	41.0
36	37.28225444944163	39.0	36.0	41.0	31.0	41.0
37	37.17458855287853	39.0	36.0	41.0	31.0	41.0
38	37.1325300611321	39.0	36.0	41.0	31.0	41.0
39	37.053127293685314	39.0	36.0	41.0	31.0	41.0
40	36.93023502115732	39.0	36.0	40.0	31.0	41.0
41	36.882612572228	39.0	36.0	40.0	31.0	41.0
42	36.820460781367764	39.0	35.0	40.0	30.0	41.0
43	36.14884262794146	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	9.0
11	7.0
12	5.0
13	12.0
14	6.0
15	5.0
16	6.0
17	6.0
18	11.0
19	29.0
20	45.0
21	76.0
22	194.0
23	329.0
24	638.0
25	1129.0
26	1738.0
27	2563.0
28	3898.0
29	5534.0
30	7506.0
31	9716.0
32	12555.0
33	16018.0
34	21172.0
35	28472.0
36	39343.0
37	57826.0
38	97671.0
39	99485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72802864982291	23.380688955335636	15.743856002128048	24.14742639271341
2	13.496352270656098	22.176765860603044	41.00284232252726	23.324039546213605
3	14.567765008398892	29.13848563814328	34.29087254868154	22.002876804776285
4	11.28111407220583	19.08567853677039	37.55215440165909	32.081052989364686
5	12.264597074920076	38.755584892834094	34.92805525041502	14.05176278183081
6	27.41905292040019	39.97724171563967	17.645059432619224	14.958645931340916
7	23.909991477958453	33.915016034245795	22.422328734058116	19.75266375373763
8	25.074998891641997	34.57362699073413	22.609025482382034	17.742348635241846
9	23.577484076590004	14.608158500120688	19.54675546666798	42.267601956621334
10	14.251759826209465	28.592434594562643	33.36428525686813	23.79152032235977
11	31.161854750915012	23.024782884981995	25.01366974872293	20.79969261538007
12	20.125318345049088	26.936055132190162	29.136515223912944	23.8021112988478
13	29.0601616724876	20.52087900178815	26.62349817490382	23.795461150820433
14	21.456086855859276	22.631438944252054	26.805515189430697	29.106959010457974
15	23.81565789668133	28.917799244346142	23.012714097821217	24.253828761151315
16	24.25506027004527	27.259941971300915	23.622064698551252	24.86293306010256
17	20.27359201588154	30.961611404757566	25.069826554287378	23.694970025073523
18	23.18044560917819	25.836071388107566	26.026462663113353	24.957020339600895
19	22.96764087230238	29.165086230252758	25.8582385481988	22.009034349246072
20	21.593769550203692	25.94419786899701	26.50970675310217	25.952325827697127
21	24.700866489657788	25.701836918666228	27.585060319305626	22.01223627237036
22	22.314694856726256	30.347088466672904	26.23877479643158	21.09944188016926
23	23.198179337251172	26.85034211317074	26.768077319054402	23.183401230523685
24	24.536829504982684	28.020521864208902	26.02843307734368	21.41421555346473
25	21.708792480899298	27.311665344847118	26.37571858543962	24.60382358881396
26	25.015393861174466	27.81880070737871	27.093934572395483	20.071870859051344
27	22.034403432461588	28.889720841563914	26.237543287537623	22.838332438436872
28	20.962744392940007	26.348871691551356	30.138224558257757	22.55015935725088
29	24.10654029743403	26.95896119761777	26.490248912577645	22.444249592370554
30	20.879494391708498	27.05181696822214	28.44711654507569	23.62157209499367
31	24.955788830706936	24.691753323842505	27.96781328354753	22.384644561903023
32	23.60728659182377	26.088777013147592	27.6059959705029	22.697940424525743
33	22.54818894302055	27.431368009339764	29.077402797003	20.94304025063669
34	22.842519568676327	28.527903528519282	26.784086934675845	21.84548996812855
35	21.217913035767943	25.371546233306898	31.307419102180756	22.1031216287444
36	24.902833948266775	25.704546238232933	25.529671975291006	23.862947838209287
37	21.952384940124038	26.479904237868407	28.64489687344522	22.922813948562336
38	21.935143815608637	25.00923631670468	28.770510780628854	24.285109087057826
39	24.044472249178583	25.71095008448151	27.244424959237058	23.00015270710285
40	20.717427821263726	24.712935276818566	32.69138879720989	21.87824810470781
41	22.30139456067152	26.053555858780413	26.770786638621104	24.87426294192697
42	21.99721186386408	25.14273188080964	30.685999714289935	22.17405654103634
43	22.88759279419516	24.399146810638268	29.956700147288462	22.75656024787811
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	7.0
2	9.0
3	47.0
4	85.0
5	85.0
6	119.0
7	153.0
8	170.5
9	188.0
10	267.5
11	347.0
12	347.0
13	586.5
14	826.0
15	1960.0
16	3094.0
17	3420.5
18	3747.0
19	3747.0
20	3677.0
21	3607.0
22	4220.0
23	4833.0
24	6147.0
25	7461.0
26	7461.0
27	8796.5
28	10132.0
29	11826.0
30	13520.0
31	15481.5
32	17443.0
33	17443.0
34	19551.0
35	21659.0
36	23063.0
37	24467.0
38	25341.5
39	26216.0
40	26216.0
41	26765.5
42	27315.0
43	27448.5
44	27582.0
45	27560.5
46	27539.0
47	27539.0
48	27108.0
49	26677.0
50	26458.0
51	26239.0
52	32352.0
53	38465.0
54	38465.0
55	30078.5
56	21692.0
57	25258.5
58	28825.0
59	23321.0
60	17817.0
61	17817.0
62	13622.0
63	9427.0
64	7583.5
65	5740.0
66	4709.0
67	3678.0
68	3678.0
69	3003.5
70	2329.0
71	1922.0
72	1515.0
73	1559.5
74	1604.0
75	1604.0
76	1335.0
77	1066.0
78	770.5
79	475.0
80	300.0
81	125.0
82	125.0
83	93.0
84	61.0
85	46.0
86	31.0
87	20.5
88	10.0
89	10.0
90	5.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	406006.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.40958701603301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39543660134247	50.73307621539585
2	8.93329715838451	10.614469897422499
3	2.183223648228071	3.8911324591459984
4	0.9023054886358545	2.144223857686239
5	0.5125871575238392	1.522629566910677
6	0.3461686187699845	1.2339440807421211
7	0.25328239726619656	1.0533181834010883
8	0.19531117855633842	0.9282685166117397
9	0.14326453845919226	0.7660158357512871
>10	1.0153735508733288	11.565885435764082
>50	0.08452917314866247	3.3939290749906523
>100	0.029419467124963992	2.8343874206953905
>500	0.0020717934595045062	0.7444144137730468
>1k	0.002486152151405408	3.3587269383331524
>5k	0.001243076075702704	5.215578103376172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	7498	1.8467707373782654	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	7021	1.7292847888947454	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	6668	1.6423402609813649	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	4387	1.0805259035580754	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2200	0.5418639133411822	Illumina Paired End PCR Primer 2 (95% over 23bp)
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	2104	0.5182189425772034	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1923	0.4736383206159515	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.46649556903099954	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1136	0.2797988207070831	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	673	0.16576109712664347	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	642	0.15812574198410861	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	588	0.1448254459293705	No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	562	0.1384215996807929	No Hit
TATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTAT	559	0.13768269434441854	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	446	0.1098505933409851	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.463017787914464E-4	0.0
12	0.0	0.0	0.0	2.463017787914464E-4	0.0
13	0.0	0.0	0.0	2.463017787914464E-4	0.0
14	0.0	0.0	0.0	2.463017787914464E-4	0.0
15	0.0	0.0	0.0	2.463017787914464E-4	0.0
16	0.0	0.0	0.0	2.463017787914464E-4	0.0
17	0.0	0.0	0.0	2.463017787914464E-4	0.0
18	0.0	0.0	0.0	2.463017787914464E-4	0.0
19	0.0	0.0	0.0	2.463017787914464E-4	0.0
20	0.0	0.0	0.0	2.463017787914464E-4	2.463017787914464E-4
21	0.0	0.0	0.0	2.463017787914464E-4	2.463017787914464E-4
22	0.0	0.0	0.0	0.001231508893957232	2.463017787914464E-4
23	0.0	0.0	0.0	0.002709319566705911	2.463017787914464E-4
24	0.0	0.0	0.0	0.00344822490308025	2.463017787914464E-4
25	0.0	0.0	0.0	0.00344822490308025	2.463017787914464E-4
26	0.0	0.0	0.0	0.00344822490308025	2.463017787914464E-4
27	2.463017787914464E-4	0.0	0.0	0.003940828460663143	2.463017787914464E-4
28	2.463017787914464E-4	0.0	0.0	0.008866864036492072	2.463017787914464E-4
29	2.463017787914464E-4	0.0	0.0	0.020935651197272948	2.463017787914464E-4
30	2.463017787914464E-4	0.0	0.0	0.03694526681871697	2.463017787914464E-4
31	2.463017787914464E-4	0.0	0.0	0.07512204253139117	2.463017787914464E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTCTA	20	0.0018405344	37.0	14
CTTATAC	965	0.0	33.165806	1
CGTCTTC	340	0.0	31.014706	37
TTATACA	1025	0.0	30.682928	2
TATACAC	1040	0.0	30.596153	3
GGTATCA	235	0.0	29.12766	1
CCGTATT	35	8.8594755E-4	26.428572	1
CCGTCTT	1020	0.0	24.84804	37
GCCGTCT	1025	0.0	24.726831	36
CCGCGTG	45	1.321154E-4	24.666666	37
GTATCAA	785	0.0	24.509554	1
GCCGCGT	55	1.8987319E-5	23.545454	36
CCGCCGT	40	0.0019290481	23.125	24
TTAGGAC	75	3.7284553E-7	22.2	3
TTTCGCC	50	2.697933E-4	22.2	3
ACGGAGT	50	2.697933E-4	22.2	23
ATGCCGC	60	3.7186506E-5	21.583332	34
TGCCGCG	60	3.7186506E-5	21.583332	35
TTACGGG	45	0.0038216114	20.555555	3
TAGGACC	45	0.0038216114	20.555555	4
>>END_MODULE