FastQCFastQC Report
Fri 10 Feb 2017
ERR1630850.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630850.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165785
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG81464.9135929064752535No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT79924.820701510993153TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC44002.6540398709171518No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC34662.090659589227011RNA PCR Primer, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT29111.7558886509635976No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC24711.4904846638718823No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18501.1159031275447115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14880.8975480290738004No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG9630.5808728172030039No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT6770.40836022559338897No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA6380.384835781282987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6160.3715655819284013No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5950.3588985734535694No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5630.33959646530144466No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4690.2828965226045782No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4560.2750550411677775No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4430.2672135597309769No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3600.21714871671140332No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3440.20749766263534092No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC3100.1869891727237084No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2740.1652743010525681No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2740.1652743010525681No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT2710.1634647284133064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.1628615375335525No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2660.1604487740145369No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2520.15200410169798234No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2340.14114666586241215No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2260.13632113882438096No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1890.11400307627348674No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1860.11219350363422505No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1830.11038393099496335No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA1830.11038393099496335No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1810.10917754923545557No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1790.10797116747594776No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT1790.10797116747594776TruSeq Adapter, Index 10 (95% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1770.10676478571643996No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1720.10374883131767046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCCG308.2691095E-637.010
AGGCCTC308.2691095E-637.05
GCCGAGC200.001837396137.03
CTCGCCC308.2691095E-637.09
CGATGTG308.2691095E-637.015
GCCCGAT308.2691095E-637.012
TGCCGCC200.001837396137.034
CTTATAC4800.035.0729141
CGTCTTC4300.033.5581437
CGCCCGA352.376087E-531.71428511
TTATACA5200.031.6634622
TTTACAC658.54925E-1131.3076922
CACGAGG303.5863192E-430.83333214
GGGTGCA250.0054829529.636
AGGGTGC250.0054829529.635
ATGTCTG250.0054829529.69
TCAGAGT250.0054829529.63
CCGAGGT250.0054829529.632
GGTGCAA250.0054829529.637
GTATAGA250.0054829529.61
Produced by FastQC (version 0.11.5)