##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630850.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	165785
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.04389420031969	31.0	30.0	31.0	26.0	34.0
2	30.014910878547518	31.0	30.0	31.0	26.0	34.0
3	29.44568567723256	31.0	30.0	31.0	25.0	34.0
4	33.626751515517086	35.0	33.0	37.0	28.0	37.0
5	33.28128600295563	35.0	33.0	37.0	28.0	37.0
6	33.606538589136534	35.0	33.0	37.0	28.0	37.0
7	33.69462255330699	35.0	33.0	37.0	29.0	37.0
8	33.74783605271888	35.0	33.0	37.0	29.0	37.0
9	35.319148294477785	37.0	34.0	39.0	30.0	39.0
10	34.97710287420454	37.0	34.0	39.0	29.0	39.0
11	35.11969116626957	37.0	34.0	39.0	30.0	39.0
12	34.963253611605396	37.0	34.0	39.0	29.0	39.0
13	35.19074101999578	37.0	34.0	39.0	30.0	39.0
14	35.73436680037398	38.0	34.0	40.0	29.0	41.0
15	35.715722170280785	38.0	34.0	40.0	29.0	41.0
16	35.77064873179118	38.0	34.0	40.0	29.0	41.0
17	35.69699309346443	38.0	34.0	40.0	29.0	41.0
18	35.83978043851977	38.0	34.0	40.0	29.0	41.0
19	35.72753264770637	38.0	34.0	40.0	28.0	41.0
20	35.73056066592273	38.0	34.0	40.0	29.0	41.0
21	35.56451428054408	38.0	34.0	40.0	27.0	41.0
22	35.37612570497934	38.0	34.0	40.0	27.0	40.0
23	35.407901800524776	38.0	34.0	40.0	27.0	40.0
24	35.2598003438188	37.0	33.0	40.0	27.0	40.0
25	35.23698163283771	37.0	33.0	40.0	27.0	41.0
26	34.82209488192539	37.0	33.0	39.0	26.0	40.0
27	34.61843954519408	37.0	33.0	39.0	25.0	40.0
28	34.50850800735893	37.0	33.0	39.0	25.0	40.0
29	34.5259824471454	37.0	33.0	39.0	25.0	40.0
30	34.321500738908824	37.0	32.0	39.0	25.0	40.0
31	34.25799680308834	37.0	32.0	39.0	25.0	40.0
32	33.913315438670566	36.0	32.0	39.0	24.0	40.0
33	33.77455740869198	36.0	31.0	39.0	24.0	40.0
34	33.6022076786199	36.0	31.0	39.0	24.0	40.0
35	33.55699852218235	36.0	31.0	39.0	23.0	40.0
36	33.613535603341674	36.0	31.0	39.0	23.0	40.0
37	33.448001930210815	36.0	31.0	39.0	23.0	40.0
38	33.30617365865428	36.0	31.0	39.0	22.0	40.0
39	33.103465331604184	36.0	31.0	39.0	22.0	40.0
40	32.62939952347921	35.0	30.0	39.0	20.0	40.0
41	32.62872998160268	35.0	30.0	39.0	20.0	40.0
42	32.38791205476973	35.0	30.0	38.0	20.0	40.0
43	31.36806104291703	34.0	28.0	38.0	17.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	10.0
11	12.0
12	6.0
13	9.0
14	5.0
15	8.0
16	14.0
17	32.0
18	43.0
19	93.0
20	169.0
21	301.0
22	526.0
23	898.0
24	1354.0
25	1989.0
26	2801.0
27	3811.0
28	5303.0
29	6546.0
30	8306.0
31	10252.0
32	12263.0
33	14795.0
34	17253.0
35	19512.0
36	20349.0
37	18357.0
38	15274.0
39	5491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.60065747805893	26.00717797146907	18.834032029435715	22.55813252103628
2	14.559218264619838	22.478511324908766	38.569834424103504	24.392435986367886
3	15.665470338088488	31.665108423560635	33.019875139487894	19.649546098862984
4	10.751877431613234	18.262810266308772	31.377386373918025	39.607925928159965
5	12.746629670959376	39.82929698102965	29.952046325059566	17.472027022951416
6	26.615194378261002	37.09261995958621	16.433935518894955	19.858250143257834
7	22.766233374551376	35.355430225894985	19.157945531863557	22.720390867690078
8	27.9301504961245	28.39822661881352	24.345387097747082	19.326235787314896
9	20.927707573061497	12.720089272250204	16.37602919443858	49.97617396024972
10	15.49416412823838	24.5414241336671	30.738004041378897	29.22640769671563
11	30.10646319027656	21.676870645715837	20.74132159121754	27.47534457279006
12	18.689869409174534	29.796423078083063	22.886268359622402	28.627439153120005
13	34.65995114153874	17.237385770727144	22.067738335796363	26.034924751937748
14	23.55942938142775	25.403987091715173	23.669813312422715	27.366770214434354
15	28.175649184184337	27.995898302017675	19.52468558675393	24.303766927044062
16	26.27378834032029	24.096269264408722	20.056096751817112	29.573845643453872
17	18.84247670175227	31.958862382000785	21.328829508097837	27.86983140814911
18	22.3524444310402	19.83110655366891	23.211388243809754	34.605060771481135
19	26.174865036040657	25.959525891968514	23.180625508942303	24.684983563048526
20	25.44198811713967	23.97683747021745	23.774768525499894	26.806405887142986
21	29.38685647073016	25.752631420212925	23.481014567059745	21.379497541997164
22	27.342642579244202	29.911632536116056	23.534095364478087	19.211629520161654
23	29.689658292366623	21.801731157824893	24.136683053352233	24.37192749645625
24	22.056277709081037	26.654401785445003	24.792351539644724	26.496968965829236
25	25.969176946044577	23.573905962541847	28.82287299816027	21.63404409325331
26	26.396236088910335	26.028892843140213	23.35313810055192	24.221732967397532
27	26.60916246946346	27.09654070030461	27.35470639683928	18.93959043339265
28	21.671441927798053	25.80752179027053	29.719817836354313	22.801218445577103
29	25.343668003739783	21.72512591609615	25.33281056790421	27.598395512259856
30	26.37150526284043	20.86980124860512	32.99695388605725	19.76173960249721
31	26.28705853967488	21.10022016467111	23.33262961064029	29.28009168501372
32	24.6656814548964	22.308411496818167	33.52957143287993	19.496335615405496
33	25.269475525530055	21.813794975419974	32.51259160961486	20.404137889435113
34	19.366649576258407	29.53343185451036	27.135748107488617	23.96417046174262
35	20.531411165063183	21.091775492354557	40.174925355128636	18.20188798745363
36	26.548843381488073	22.236631782127457	23.350725337032905	27.863799499351572
37	21.5007389088277	21.859034291401514	30.561269113611	26.078957686159786
38	21.91694061585789	20.403534698555358	27.893959043339265	29.78556564224749
39	28.698615676930967	22.088246825707994	26.63811563169165	22.57502186566939
40	18.99267123081099	20.931326718340017	37.84298941400006	22.23301263684893
41	23.891184365292396	23.601652743010526	25.359954157493135	27.147208734203936
42	19.832312935428416	21.00732876918901	36.726483095575595	22.43387519980698
43	24.96426094037458	19.95536387489821	30.449678800856532	24.630696383870678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	5.5
2	10.0
3	21.0
4	32.0
5	32.0
6	73.0
7	114.0
8	127.0
9	140.0
10	211.5
11	283.0
12	283.0
13	489.0
14	695.0
15	1704.5
16	2714.0
17	2636.0
18	2558.0
19	2558.0
20	2157.0
21	1756.0
22	1396.0
23	1036.0
24	1035.5
25	1035.0
26	1035.0
27	1113.0
28	1191.0
29	1521.5
30	1852.0
31	2315.5
32	2779.0
33	2779.0
34	3646.0
35	4513.0
36	5059.5
37	5606.0
38	6313.5
39	7021.0
40	7021.0
41	7774.5
42	8528.0
43	9214.0
44	9900.0
45	10949.5
46	11999.0
47	11999.0
48	12790.0
49	13581.0
50	13022.0
51	12463.0
52	12878.5
53	13294.0
54	13294.0
55	17682.5
56	22071.0
57	15145.5
58	8220.0
59	13216.5
60	18213.0
61	18213.0
62	14498.5
63	10784.0
64	6295.0
65	1806.0
66	1367.5
67	929.0
68	929.0
69	669.5
70	410.0
71	296.5
72	183.0
73	112.0
74	41.0
75	41.0
76	25.5
77	10.0
78	10.5
79	11.0
80	5.5
81	0.0
82	0.0
83	2.0
84	4.0
85	2.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	165785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.220888500165874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54550506561792	20.75519498145188
2	9.720856666524174	5.486624242241457
3	4.291882186979011	3.6336218596374823
4	2.635403753259522	2.974937418946226
5	1.7932714914718078	2.5303857405676027
6	1.1413670756209122	1.9326235787314894
7	0.8870174838626941	1.7522695056850741
8	0.6946522463984953	1.5682962873601354
9	0.5514470140640363	1.4006092227885514
>10	4.2598213140683105	23.382694453659862
>50	0.33129568674389775	6.34737762765027
>100	0.1175565340058992	6.052417287450614
>500	0.012824349164279911	2.4441294447627953
>1k	0.012824349164279911	10.004523931598154
>5k	0.004274783054759972	9.734294417468407
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	8146	4.9135929064752535	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	7992	4.820701510993153	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	4400	2.6540398709171518	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	3466	2.090659589227011	RNA PCR Primer, Index 10 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	2911	1.7558886509635976	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	2471	1.4904846638718823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	1.1159031275447115	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1488	0.8975480290738004	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	963	0.5808728172030039	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	677	0.40836022559338897	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	638	0.384835781282987	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	616	0.3715655819284013	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	595	0.3588985734535694	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	563	0.33959646530144466	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	469	0.2828965226045782	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	456	0.2750550411677775	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	443	0.2672135597309769	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	360	0.21714871671140332	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	344	0.20749766263534092	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	310	0.1869891727237084	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	274	0.1652743010525681	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	274	0.1652743010525681	No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT	271	0.1634647284133064	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	270	0.1628615375335525	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	266	0.1604487740145369	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	252	0.15200410169798234	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	234	0.14114666586241215	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	226	0.13632113882438096	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	189	0.11400307627348674	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	186	0.11219350363422505	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	183	0.11038393099496335	No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	183	0.11038393099496335	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	181	0.10917754923545557	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	179	0.10797116747594776	No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT	179	0.10797116747594776	TruSeq Adapter, Index 10 (95% over 23bp)
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	177	0.10676478571643996	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	172	0.10374883131767046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0012063817595077961	0.0
23	0.0	0.0	0.0	0.0024127635190155923	0.0
24	0.0	0.0	0.0	0.004222336158277287	0.0
25	0.0	0.0	0.0	0.004222336158277287	0.0
26	0.0	0.0	0.0	0.004222336158277287	0.0
27	0.0	0.0	0.0	0.004222336158277287	0.0
28	0.0	0.0	0.0	0.007841481436800676	0.0
29	0.0	0.0	0.0	0.01869891727237084	0.0
30	0.0	0.0	0.0	0.0337786892662183	0.0
31	0.0	0.0	0.0	0.062128660614651506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	30	8.2691095E-6	37.0	10
AGGCCTC	30	8.2691095E-6	37.0	5
GCCGAGC	20	0.0018373961	37.0	3
CTCGCCC	30	8.2691095E-6	37.0	9
CGATGTG	30	8.2691095E-6	37.0	15
GCCCGAT	30	8.2691095E-6	37.0	12
TGCCGCC	20	0.0018373961	37.0	34
CTTATAC	480	0.0	35.072914	1
CGTCTTC	430	0.0	33.55814	37
CGCCCGA	35	2.376087E-5	31.714285	11
TTATACA	520	0.0	31.663462	2
TTTACAC	65	8.54925E-11	31.307692	2
CACGAGG	30	3.5863192E-4	30.833332	14
GGGTGCA	25	0.00548295	29.6	36
AGGGTGC	25	0.00548295	29.6	35
ATGTCTG	25	0.00548295	29.6	9
TCAGAGT	25	0.00548295	29.6	3
CCGAGGT	25	0.00548295	29.6	32
GGTGCAA	25	0.00548295	29.6	37
GTATAGA	25	0.00548295	29.6	1
>>END_MODULE