FastQCFastQC Report
Fri 10 Feb 2017
ERR1630849.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630849.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592594
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT118441.9986702531581488No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG111821.8869580184747061No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT83271.4051779127024573TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC63801.0766224430216977No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC31260.527511247160788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25740.4343614683915126No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC24950.42103025005315614RNA PCR Primer, Index 7 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24470.41293026929061044No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG16490.2782680891132884No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13620.2298369541372339No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT11630.19625578389251327No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC10840.18292456555415681No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC10840.18292456555415681No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9730.1641933600407699No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT9720.1640246104415502No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA9640.1626746136477926No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC9590.16183086565169408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9090.15339338569070898No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT8940.15086214170241347No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG8830.14900589611099674No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT8360.14107466494767074No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA8220.13871217055859494No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT7530.1270684482124355No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7350.12403095542648085No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC7300.12318720743038235No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT6870.11593097466393518No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6820.11508722666783668No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA6740.11373722987407905No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6310.10648099710763187No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC6230.10513100031387425No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6020.1015872587302605No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC6010.10141850913104082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC12900.034.4186021
CGTCTTC3800.031.15789637
ACCGCTC303.5973926E-430.83333215
ATTACGG303.5973926E-430.8333322
GATTACG303.5973926E-430.8333321
TTATACA14750.030.227122
GCGGTCC250.005494220629.620
CGGTCCG250.005494220629.621
TATACAC15450.029.2168273
TTACGGG405.935348E-527.753
GGTATCA2550.027.5686261
CCGTCTT12500.026.04837
GTATCAA10250.025.9902441
GCCGTCT13050.024.95019336
TAGAGTG400.001930089223.1255
CGCTCCA502.6999612E-422.217
ACGTGGG1800.020.55555736
CGTAAAC450.00382365320.5555573
GGGATAG450.00382365320.5555575
TGCCGTC16100.020.22360235
Produced by FastQC (version 0.11.5)