##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630849.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	592594
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49906175222834	31.0	31.0	34.0	28.0	34.0
2	31.51079828685407	31.0	31.0	34.0	30.0	34.0
3	30.98534409730777	31.0	31.0	34.0	27.0	34.0
4	35.11272304478277	37.0	35.0	37.0	32.0	37.0
5	35.30775539408094	37.0	35.0	37.0	33.0	37.0
6	35.49809819201679	37.0	35.0	37.0	33.0	37.0
7	35.50572061141355	37.0	35.0	37.0	33.0	37.0
8	35.51816251936402	37.0	35.0	37.0	33.0	37.0
9	37.2991559145047	39.0	37.0	39.0	34.0	39.0
10	37.141628163633115	39.0	37.0	39.0	33.0	39.0
11	37.18682774378411	39.0	37.0	39.0	33.0	39.0
12	37.12492195331036	39.0	37.0	39.0	33.0	39.0
13	37.18333800207225	39.0	37.0	39.0	33.0	39.0
14	38.303629466380016	40.0	38.0	41.0	33.0	41.0
15	38.30682052130126	40.0	38.0	41.0	33.0	41.0
16	38.313177318703865	40.0	38.0	41.0	33.0	41.0
17	38.29654367070878	40.0	38.0	41.0	33.0	41.0
18	38.34964073210326	40.0	38.0	41.0	34.0	41.0
19	38.37246242790173	40.0	38.0	41.0	34.0	41.0
20	38.365415444638316	40.0	38.0	41.0	34.0	41.0
21	38.33009615352164	40.0	38.0	41.0	34.0	41.0
22	38.22683321127112	40.0	38.0	41.0	34.0	41.0
23	38.262442751698465	40.0	38.0	41.0	34.0	41.0
24	38.20827244285295	40.0	38.0	41.0	33.0	41.0
25	38.17935888652264	40.0	38.0	41.0	33.0	41.0
26	38.09648258335386	40.0	37.0	41.0	33.0	41.0
27	37.97396362433639	40.0	37.0	41.0	33.0	41.0
28	38.01479427736359	40.0	37.0	41.0	33.0	41.0
29	37.9365265257495	40.0	37.0	41.0	33.0	41.0
30	37.844075370320994	40.0	37.0	41.0	33.0	41.0
31	37.83643101347634	40.0	37.0	41.0	33.0	41.0
32	37.70245395667185	40.0	37.0	41.0	33.0	41.0
33	37.691777169529225	40.0	37.0	41.0	33.0	41.0
34	37.637336861324954	40.0	37.0	41.0	32.0	41.0
35	37.62102721256037	40.0	37.0	41.0	33.0	41.0
36	37.58169843096623	40.0	37.0	41.0	32.0	41.0
37	37.50728998268629	40.0	37.0	41.0	32.0	41.0
38	37.44616381536094	40.0	37.0	41.0	32.0	41.0
39	37.34904504601801	39.0	36.0	41.0	32.0	41.0
40	37.25278858712711	39.0	36.0	41.0	31.0	41.0
41	37.22139272419228	39.0	36.0	41.0	31.0	41.0
42	37.150565142407785	39.0	36.0	41.0	31.0	41.0
43	36.466530204490766	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	7.0
11	9.0
12	11.0
13	8.0
14	14.0
15	7.0
16	9.0
17	11.0
18	16.0
19	23.0
20	57.0
21	117.0
22	237.0
23	408.0
24	764.0
25	1296.0
26	2155.0
27	3414.0
28	4980.0
29	7035.0
30	9826.0
31	13087.0
32	17117.0
33	22031.0
34	28967.0
35	38949.0
36	53770.0
37	79768.0
38	137376.0
39	171115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.11124311079761	23.38531945986628	15.705018950579994	23.798418478756115
2	13.048056510865786	22.663577424003616	42.02810018326206	22.26026588186853
3	14.313678505013549	29.165499481938728	35.679402761418444	20.84141925162928
4	11.389922948932995	19.5148786521632	36.92072481327857	32.174473585625236
5	11.914734202506269	39.36877524915878	35.24791003621367	13.468580512121283
6	26.710530312490512	39.8211929246668	18.061944602881567	15.406332159961119
7	22.981839168131977	34.30932476535368	22.472046628889256	20.236789437625085
8	24.170173845837116	35.434209593752215	22.97373918736943	17.42187737304124
9	23.06975770932544	15.44632581497619	19.344947805748962	42.13896866994941
10	14.130247690661735	29.325474101999006	33.38963944960631	23.15463875773295
11	30.438377708852943	23.14400753298211	25.614839164756987	20.802775593407965
12	19.641609601177198	27.25576026756936	28.839475256246267	24.263154875007174
13	28.597150831766776	20.864537946722375	27.499265939243394	23.039045282267452
14	21.025693813977192	23.326594599337827	26.639824230417453	29.00788735626753
15	23.8154621882773	28.80285659321559	23.506312922506808	23.875368296000296
16	24.319517241146553	27.860221331974337	23.13253256023517	24.68772886664394
17	20.543238709808065	31.30035066166718	24.62495401573421	23.531456612790542
18	22.910626837261262	25.7434263593624	26.621430524102507	24.724516279273836
19	23.315119626590885	28.19755178081452	27.68286550319443	20.804463089400162
20	22.620715025801815	26.913536080351808	26.67981788543252	23.785931008413854
21	24.944734506255546	25.903400979422674	26.202594018839203	22.949270495482573
22	20.186164557859176	31.789049501007433	25.903063480224237	22.121722460909155
23	24.019817952932364	25.79219499353689	26.30300003037493	23.88498702315582
24	23.16746372727365	27.90797746855351	26.98829215280614	21.936266651366704
25	21.978622800770847	28.37271386480457	27.29761016817585	22.35105316624873
26	23.74492485580347	25.59982045042643	27.614353165911233	23.04090152785887
27	20.69291960431594	30.53692747479725	27.15788550002194	21.612267420864875
28	22.207784756511202	26.078900562611164	28.07858331336463	23.634731367513
29	20.704394577062878	26.57283063952723	28.077064566971654	24.645710216438236
30	21.907072970701694	28.204639263981747	29.005356112279234	20.882931653037325
31	23.88903701353709	25.212202621018776	25.723682656253693	25.17507770919044
32	21.11175610957924	26.54397445806066	29.467561264542	22.8767081678181
33	22.21132849809482	25.452164551109192	29.10964336459701	23.22686358619898
34	19.919371441492824	28.819394053939124	26.90290485560097	24.358329648967082
35	20.606688559114673	26.101850508105045	32.772859664458295	20.518601268321987
36	22.057260114007228	26.43918095694523	25.62293914551953	25.880619783528015
37	22.382440591703595	26.97074219448729	28.151820639425985	22.494996574383137
38	21.93441040577529	25.41031465050271	28.817706557946927	23.837568385775086
39	23.854949594494713	25.974275811094948	27.16092299280789	23.009851601602445
40	20.787588129478195	25.109771614292413	32.303904528226745	21.798735728002647
41	22.10771624417392	26.71407405407412	26.8269675359521	24.351242165799857
42	22.807858331336465	24.870822181797315	30.108809741576863	22.212509745289356
43	22.537015224588842	24.904234602442816	29.7254106521497	22.833339520818637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	7.0
2	9.0
3	69.5
4	130.0
5	130.0
6	161.0
7	192.0
8	217.5
9	243.0
10	357.0
11	471.0
12	471.0
13	760.0
14	1049.0
15	2810.0
16	4571.0
17	4941.5
18	5312.0
19	5312.0
20	5342.5
21	5373.0
22	6420.0
23	7467.0
24	9101.5
25	10736.0
26	10736.0
27	13194.0
28	15652.0
29	19559.5
30	23467.0
31	24413.0
32	25359.0
33	25359.0
34	27821.5
35	30284.0
36	31267.0
37	32250.0
38	35137.0
39	38024.0
40	38024.0
41	40634.0
42	43244.0
43	39894.5
44	36545.0
45	38228.5
46	39912.0
47	39912.0
48	41025.5
49	42139.0
50	43096.0
51	44053.0
52	55223.0
53	66393.0
54	66393.0
55	48618.0
56	30843.0
57	34661.5
58	38480.0
59	30370.0
60	22260.0
61	22260.0
62	16402.0
63	10544.0
64	8632.0
65	6720.0
66	5635.0
67	4550.0
68	4550.0
69	3666.0
70	2782.0
71	2250.5
72	1719.0
73	1298.5
74	878.0
75	878.0
76	662.5
77	447.0
78	354.5
79	262.0
80	195.0
81	128.0
82	128.0
83	88.0
84	48.0
85	36.5
86	25.0
87	20.5
88	16.0
89	16.0
90	13.5
91	11.0
92	6.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	592594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.447696631891596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90594659850639	36.76988916724939
2	11.166566109131617	10.149894178955515
3	3.1276116072078723	4.264282305202989
4	1.339104416845325	2.4343684518084974
5	0.7149281150187093	1.62459180424902
6	0.43506651875003555	1.1863662695306876
7	0.3271447914080033	1.040758406423007
8	0.23676655048065165	0.8608395487059283
9	0.18924660068714413	0.7740739886981448
>10	1.233321380757339	11.123539417844162
>50	0.17199273214107696	5.458840633142996
>100	0.13633117199313421	11.551287643970618
>500	0.011144237492626774	3.5235808365459547
>1k	0.0033432712477880324	2.8673516807003256
>5k	7.429491661751183E-4	2.4829334178085074
>10k+	7.429491661751183E-4	3.887402249164254
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	11844	1.9986702531581488	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	11182	1.8869580184747061	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	8327	1.4051779127024573	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	6380	1.0766224430216977	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	3126	0.527511247160788	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2574	0.4343614683915126	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2495	0.42103025005315614	RNA PCR Primer, Index 7 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2447	0.41293026929061044	No Hit
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1649	0.2782680891132884	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1362	0.2298369541372339	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1163	0.19625578389251327	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1084	0.18292456555415681	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1084	0.18292456555415681	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	973	0.1641933600407699	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	972	0.1640246104415502	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	964	0.1626746136477926	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	959	0.16183086565169408	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	909	0.15339338569070898	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	894	0.15086214170241347	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	883	0.14900589611099674	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	836	0.14107466494767074	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	822	0.13871217055859494	No Hit
ACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT	753	0.1270684482124355	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	735	0.12403095542648085	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	730	0.12318720743038235	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	687	0.11593097466393518	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	682	0.11508722666783668	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	674	0.11373722987407905	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	631	0.10648099710763187	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	623	0.10513100031387425	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	602	0.1015872587302605	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	601	0.10141850913104082	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.6874959921970185E-4	0.0
11	0.0	0.0	0.0	1.6874959921970185E-4	0.0
12	0.0	0.0	0.0	1.6874959921970185E-4	0.0
13	0.0	0.0	0.0	1.6874959921970185E-4	0.0
14	0.0	0.0	0.0	1.6874959921970185E-4	0.0
15	0.0	0.0	0.0	3.374991984394037E-4	0.0
16	0.0	0.0	0.0	3.374991984394037E-4	0.0
17	0.0	0.0	0.0	3.374991984394037E-4	0.0
18	0.0	0.0	0.0	3.374991984394037E-4	0.0
19	0.0	0.0	0.0	3.374991984394037E-4	0.0
20	0.0	0.0	0.0	5.062487976591056E-4	0.0
21	0.0	0.0	0.0	0.0015187463929773168	0.0
22	0.0	0.0	0.0	0.0016874959921970186	0.0
23	0.0	0.0	0.0	0.002193744789856124	0.0
24	0.0	0.0	0.0	0.0025312439882955277	0.0
25	0.0	0.0	0.0	0.0028687431867349313	0.0
26	0.0	0.0	0.0	0.0032062423851743353	0.0
27	0.0	0.0	0.0	0.003543741583613739	0.0
28	0.0	0.0	0.0	0.006918733568007776	0.0
29	0.0	0.0	0.0	0.016874959921970186	0.0
30	0.0	0.0	0.0	0.026662436676712892	0.0
31	0.0	0.0	0.0	0.059568608524554756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1290	0.0	34.418602	1
CGTCTTC	380	0.0	31.157896	37
ACCGCTC	30	3.5973926E-4	30.833332	15
ATTACGG	30	3.5973926E-4	30.833332	2
GATTACG	30	3.5973926E-4	30.833332	1
TTATACA	1475	0.0	30.22712	2
GCGGTCC	25	0.0054942206	29.6	20
CGGTCCG	25	0.0054942206	29.6	21
TATACAC	1545	0.0	29.216827	3
TTACGGG	40	5.935348E-5	27.75	3
GGTATCA	255	0.0	27.568626	1
CCGTCTT	1250	0.0	26.048	37
GTATCAA	1025	0.0	25.990244	1
GCCGTCT	1305	0.0	24.950193	36
TAGAGTG	40	0.0019300892	23.125	5
CGCTCCA	50	2.6999612E-4	22.2	17
ACGTGGG	180	0.0	20.555557	36
CGTAAAC	45	0.003823653	20.555557	3
GGGATAG	45	0.003823653	20.555557	5
TGCCGTC	1610	0.0	20.223602	35
>>END_MODULE