FastQCFastQC Report
Fri 10 Feb 2017
ERR1630848.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630848.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136401
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT132209.69201105563742No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT132059.681014068811812RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG120678.84670933497555No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC64794.749965176208386No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC55324.055688741284888RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC38232.8027653756204134RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG14251.0447137484329294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14181.0395818212476446No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12830.9406089398171568No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA12780.9369432775419535No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT9950.7294667927654489No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT8380.6143649973240666No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7120.5219903079889444No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT5440.3988240555421148Illumina PCR Primer Index 11 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5100.3738975520707326No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4050.29691864429146414No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT4000.2932529820162609Illumina PCR Primer Index 11 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT3320.2433999750734965No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3220.23606865052309No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3160.2316698557928461No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3060.22433853124243958No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2950.2162740742369924No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2670.19574636549585414No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2560.18768190849040697No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2480.18181684885008176No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2300.16862046465935002No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC2130.15615721292365892No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA2040.14955902082829303No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1880.1378289015476426No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1810.13269697436235806RNA PCR Primer, Index 39 (95% over 23bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG1800.1319638419073174No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1770.12976444454219543No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG1710.12536564981195153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.12316625244682958No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1670.12243311999178892No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1660.12169998753674825No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG1620.11876745771658564No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1590.1165680603514637No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG1580.11583492789642305No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG1570.11510179544138238No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG1570.11510179544138238No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1560.11436866298634174No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT1540.11290239807626044No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA1530.11216926562121979No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG1470.10777047089097587No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1430.10483794107081325No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1420.1041048086157726No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1410.10337167616073197No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1390.10190541125065067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACAC403.7911377E-837.018
CTTATAC13600.036.455881
TTATACA15250.032.5114752
CGTCTTC7500.031.81999837
GGTATCA1200.030.8333321
TATACAC16450.030.4772053
GACAGAC551.830449E-830.27272816
CTTCTAC250.00547958629.61
CTACACA453.97431E-628.7777774
ACAGACA604.2644388E-827.74999817
GTATCAA4600.027.3478261
GTCTTCT1150.027.34782637
GACACAT556.1940045E-726.9090920
AGACAGA659.265568E-825.61538315
CCGTCTT21450.024.06293737
GCCGTCT22050.023.49206436
GAGACAG753.6910023E-722.214
GGAGATG450.003808804620.5555552
CGGAGAT450.003808804620.5555551
TGCCGTC25700.020.08365635
Produced by FastQC (version 0.11.5)