##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630848.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	136401
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16483750119134	31.0	31.0	34.0	28.0	34.0
2	31.29449197586528	31.0	31.0	34.0	28.0	34.0
3	30.78320540171993	31.0	31.0	34.0	27.0	34.0
4	34.84692927471206	37.0	35.0	37.0	32.0	37.0
5	35.01574768513427	35.0	35.0	37.0	32.0	37.0
6	35.17218348839085	37.0	35.0	37.0	32.0	37.0
7	35.12279968621931	37.0	35.0	37.0	32.0	37.0
8	35.200695009567376	37.0	35.0	37.0	32.0	37.0
9	37.05321075358685	39.0	37.0	39.0	33.0	39.0
10	36.88968555949003	39.0	37.0	39.0	33.0	39.0
11	36.88341727699944	39.0	37.0	39.0	33.0	39.0
12	36.721959516425834	39.0	35.0	39.0	32.0	39.0
13	36.864179881379165	39.0	37.0	39.0	32.0	39.0
14	37.57022309220607	39.0	37.0	41.0	32.0	41.0
15	37.554629364887354	39.0	36.0	41.0	32.0	41.0
16	37.515934633910305	39.0	36.0	41.0	32.0	41.0
17	37.35002675933461	39.0	36.0	40.0	31.0	41.0
18	37.67824282813176	39.0	37.0	41.0	32.0	41.0
19	37.60160849260636	39.0	37.0	41.0	32.0	41.0
20	37.67462848512841	39.0	37.0	41.0	32.0	41.0
21	37.65123422848806	39.0	37.0	41.0	32.0	41.0
22	37.34608983805104	39.0	36.0	40.0	32.0	41.0
23	37.51701233861922	39.0	36.0	41.0	32.0	41.0
24	37.25013746233532	39.0	36.0	40.0	31.0	41.0
25	37.16510876019971	39.0	36.0	40.0	31.0	41.0
26	37.096377592539646	39.0	36.0	40.0	31.0	41.0
27	36.80877706175175	39.0	35.0	40.0	30.0	41.0
28	37.07622378135058	39.0	36.0	40.0	31.0	41.0
29	37.01570369718697	39.0	35.0	40.0	31.0	41.0
30	36.94071157836086	39.0	35.0	40.0	31.0	41.0
31	36.913915587129125	39.0	35.0	40.0	31.0	41.0
32	36.534974083767715	38.0	35.0	40.0	30.0	41.0
33	36.40743103056429	38.0	35.0	40.0	30.0	41.0
34	36.30909597436969	38.0	35.0	40.0	30.0	41.0
35	36.32642722560685	38.0	35.0	40.0	30.0	41.0
36	36.30583353494476	38.0	35.0	40.0	30.0	41.0
37	36.22196318208811	38.0	35.0	40.0	30.0	41.0
38	36.13806350393325	38.0	35.0	40.0	30.0	41.0
39	35.79152645508464	38.0	35.0	40.0	29.0	41.0
40	35.323260093401075	38.0	34.0	40.0	26.0	41.0
41	35.390576315422905	38.0	34.0	40.0	26.0	41.0
42	35.25219023320943	38.0	34.0	40.0	26.0	41.0
43	34.267864605098204	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	12.0
11	13.0
12	10.0
13	7.0
14	7.0
15	11.0
16	6.0
17	13.0
18	14.0
19	20.0
20	27.0
21	68.0
22	110.0
23	227.0
24	367.0
25	578.0
26	997.0
27	1432.0
28	1955.0
29	2860.0
30	3505.0
31	4463.0
32	5636.0
33	6781.0
34	8673.0
35	11166.0
36	14469.0
37	20315.0
38	28768.0
39	23883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.081934883175343	25.03354080981811	20.734452093459726	30.15007221354682
2	8.664159353670428	18.10910477195915	45.82737663213613	27.399359242234294
3	10.698601916408238	31.20504981635032	40.899260269352865	17.19708799788858
4	6.9618257930660326	22.150130864143225	21.817288729554768	49.07075461323597
5	12.555626425026208	36.05472100644423	30.351683638683003	21.037968929846556
6	25.215357658668193	36.02759510560773	14.52408706681036	24.232960168913717
7	18.596637854561184	37.23139859678448	12.126743938827428	32.04521960982691
8	28.33703565223129	27.096575538302503	25.379579328597295	19.18680948086891
9	15.179507481616703	10.865756116157506	11.2858410128958	62.66889538932999
10	13.59887390854906	25.859047954193887	25.680896767619004	34.86118136963805
11	24.576799290327784	17.672157828754923	22.853938020982252	34.89710485993505
12	14.404586476638734	29.106824730023973	16.13331280562459	40.3552759877127
13	36.63682817574651	12.194192124691169	23.19851027485136	27.970469424710963
14	24.253487877654855	27.898622444116977	16.15310738191069	31.694782296317474
15	29.639078892383488	25.73074977456177	13.33347995982434	31.296691373230402
16	35.996803542496025	20.18680214954436	13.01749987170182	30.7988944362578
17	14.68024427973402	41.663184287505224	13.606205233099464	30.050366199661294
18	27.488801401749253	12.518236669819135	15.885514035820853	44.10744789261076
19	20.67140270232623	24.96682575640941	19.903079889443624	34.458691651820736
20	15.406045410224264	17.058525963885895	26.493940660259092	41.04148796563075
21	28.329704327680883	13.859869062543531	20.428002727252732	37.38242388252285
22	13.200782985461984	37.66028108298326	29.377350605933973	19.76158532562078
23	23.300415686102006	13.508698616579057	21.361280342519485	41.829605354799455
24	23.343670500949408	26.681622568749496	30.44039266574292	19.534314264558176
25	13.51236427885426	35.29226325320195	22.04162726079721	29.153745207146574
26	23.367130739510706	18.14282886489102	20.160409381162893	38.32963101443538
27	12.894333619254992	35.88903307160505	35.88756680669496	15.329066502444997
28	11.079097660574336	14.090072653426294	28.851694635669826	45.979135050329546
29	12.555626425026208	16.5248055366163	35.30545963739269	35.6141084009648
30	11.96105600398824	18.346639687392322	41.03708917090051	28.65521513771893
31	18.61276676857208	12.477914384791902	27.252732751226166	41.65658609540986
32	21.382541183715663	27.32677912918527	35.82085175328626	15.469827933812802
33	12.419996920843689	17.580516271874842	34.058401331368536	35.941085475912935
34	14.408252138913937	33.30840682986195	32.16032140526829	20.12301962595582
35	16.6582356434337	25.026942617722742	43.45349374271449	14.861327996129061
36	23.692641549548757	16.98594585083687	17.518933145651424	41.802479453962945
37	18.711739650002563	23.974897544739406	29.558434322329013	27.75492848292901
38	19.65748051700501	13.57101487525751	32.02835756336097	34.743147044376514
39	32.67498038870683	16.78360129324565	22.925785001576234	27.61563331647129
40	14.377460575802232	13.382599834312064	50.54728337768785	21.692656212197857
41	22.672854304587208	20.612752105922976	21.32535685222249	35.38903673726732
42	23.418450011363554	13.424388384249383	43.24308472811783	19.914076876269235
43	23.658184324161844	13.472775126282066	37.63828710933204	25.230753440224046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	24.5
4	45.0
5	45.0
6	51.0
7	57.0
8	85.5
9	114.0
10	191.0
11	268.0
12	268.0
13	383.5
14	499.0
15	1305.5
16	2112.0
17	2107.5
18	2103.0
19	2103.0
20	1713.5
21	1324.0
22	1035.0
23	746.0
24	764.5
25	783.0
26	783.0
27	754.0
28	725.0
29	737.0
30	749.0
31	803.0
32	857.0
33	857.0
34	1008.0
35	1159.0
36	1219.0
37	1279.0
38	1342.5
39	1406.0
40	1406.0
41	1655.5
42	1905.0
43	2484.0
44	3063.0
45	4097.0
46	5131.0
47	5131.0
48	7987.0
49	10843.0
50	22603.0
51	34363.0
52	22216.0
53	10069.0
54	10069.0
55	20055.0
56	30041.0
57	23965.0
58	17889.0
59	10867.5
60	3846.0
61	3846.0
62	3078.5
63	2311.0
64	1820.5
65	1330.0
66	1054.5
67	779.0
68	779.0
69	572.0
70	365.0
71	263.5
72	162.0
73	103.5
74	45.0
75	45.0
76	29.0
77	13.0
78	9.5
79	6.0
80	6.0
81	6.0
82	6.0
83	3.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	136401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.12229382482533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.67634091170494	14.088606388516215
2	10.53176398420427	4.027829707993344
3	4.443507265268566	2.5491015461763475
4	2.438369819422612	1.8650889656234193
5	1.5258980945443392	1.4589335855308978
6	1.131004869071809	1.2976444454219545
7	0.8549630027220796	1.144419762318458
8	0.5750872215619369	0.8797589460487827
9	0.4447341180078978	0.765390283062441
>10	3.7994095771191962	14.473500927412555
>50	0.31821492926427175	4.172256801636352
>100	0.20319748495188436	6.8489233949897725
>500	0.019169574052064563	2.6385437056913075
>1k	0.019169574052064563	6.764613162660098
>5k	0.007667829620825825	8.805653917493274
>10k+	0.011501744431238739	28.219734459424785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	13220	9.69201105563742	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	13205	9.681014068811812	RNA PCR Primer, Index 39 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	12067	8.84670933497555	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	6479	4.749965176208386	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	5532	4.055688741284888	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3823	2.8027653756204134	RNA PCR Primer, Index 39 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	1425	1.0447137484329294	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	1.0395818212476446	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1283	0.9406089398171568	No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	1278	0.9369432775419535	No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT	995	0.7294667927654489	No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	838	0.6143649973240666	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	712	0.5219903079889444	No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT	544	0.3988240555421148	Illumina PCR Primer Index 11 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	510	0.3738975520707326	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	405	0.29691864429146414	No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT	400	0.2932529820162609	Illumina PCR Primer Index 11 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	332	0.2433999750734965	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	322	0.23606865052309	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	316	0.2316698557928461	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	306	0.22433853124243958	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	295	0.2162740742369924	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	267	0.19574636549585414	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	256	0.18768190849040697	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	248	0.18181684885008176	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	230	0.16862046465935002	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	213	0.15615721292365892	No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA	204	0.14955902082829303	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	188	0.1378289015476426	No Hit
TCGCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	181	0.13269697436235806	RNA PCR Primer, Index 39 (95% over 23bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	180	0.1319638419073174	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	177	0.12976444454219543	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	171	0.12536564981195153	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.12316625244682958	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	167	0.12243311999178892	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	166	0.12169998753674825	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	162	0.11876745771658564	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	159	0.1165680603514637	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	158	0.11583492789642305	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	157	0.11510179544138238	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	157	0.11510179544138238	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	156	0.11436866298634174	No Hit
CTGATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	154	0.11290239807626044	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	153	0.11216926562121979	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	147	0.10777047089097587	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	143	0.10483794107081325	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	142	0.1041048086157726	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	141	0.10337167616073197	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	139	0.10190541125065067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.331324550406522E-4	0.0
10	0.0	0.0	0.0	7.331324550406522E-4	0.0
11	0.0	0.0	0.0	7.331324550406522E-4	0.0
12	0.0	0.0	0.0	7.331324550406522E-4	0.0
13	0.0	0.0	0.0	7.331324550406522E-4	0.0
14	0.0	0.0	0.0	7.331324550406522E-4	0.0
15	0.0	0.0	0.0	7.331324550406522E-4	0.0
16	0.0	0.0	0.0	7.331324550406522E-4	0.0
17	0.0	0.0	0.0	7.331324550406522E-4	0.0
18	0.0	0.0	0.0	7.331324550406522E-4	0.0
19	0.0	0.0	0.0	7.331324550406522E-4	0.0
20	0.0	0.0	0.0	0.0014662649100813045	0.0
21	0.0	0.0	0.0	0.0014662649100813045	0.0
22	0.0	0.0	0.0	0.0021993973651219566	0.0
23	0.0	0.0	0.0	0.0021993973651219566	0.0
24	0.0	0.0	0.0	0.0021993973651219566	0.0
25	0.0	0.0	0.0	0.0021993973651219566	0.0
26	0.0	0.0	0.0	0.002932529820162609	0.0
27	0.0	0.0	0.0	0.005131927185284565	0.0
28	0.0	0.0	0.0	0.01612891401089435	0.0
29	0.0	0.0	0.0	0.03812288766211391	0.0
30	0.0	0.0	0.0	0.05938372885829283	0.0
31	0.0	0.0	0.0	0.10043914634056936	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGACAC	40	3.7911377E-8	37.0	18
CTTATAC	1360	0.0	36.45588	1
TTATACA	1525	0.0	32.511475	2
CGTCTTC	750	0.0	31.819998	37
GGTATCA	120	0.0	30.833332	1
TATACAC	1645	0.0	30.477205	3
GACAGAC	55	1.830449E-8	30.272728	16
CTTCTAC	25	0.005479586	29.6	1
CTACACA	45	3.97431E-6	28.777777	4
ACAGACA	60	4.2644388E-8	27.749998	17
GTATCAA	460	0.0	27.347826	1
GTCTTCT	115	0.0	27.347826	37
GACACAT	55	6.1940045E-7	26.90909	20
AGACAGA	65	9.265568E-8	25.615383	15
CCGTCTT	2145	0.0	24.062937	37
GCCGTCT	2205	0.0	23.492064	36
GAGACAG	75	3.6910023E-7	22.2	14
GGAGATG	45	0.0038088046	20.555555	2
CGGAGAT	45	0.0038088046	20.555555	1
TGCCGTC	2570	0.0	20.083656	35
>>END_MODULE