FastQCFastQC Report
Fri 10 Feb 2017
ERR1630847.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630847.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153409
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG105676.888122600368948No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT91885.989218363981252RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC65154.246817331447308No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT55013.585839161978763No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC32562.1224308873664515No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC31592.05920122026739RNA PCR Primer, Index 47 (95% over 23bp)
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG14730.9601783467723536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.6218670351804653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8060.5253929039365357No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT7990.520829938269593No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA7890.5143114158882465No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT7880.5136595636501118No Hit
ATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCT3330.21706679529884168No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3190.20794086396495642No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2750.17925936548703142No Hit
CTTTACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA2040.13297785657947056No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1960.1277630386743933No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1840.11994081181677736No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT1790.11668155062610408No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAA1680.10951117600662283No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1570.10234080138714156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTATG200.001836969337.04
CTTATAC7300.035.7328761
GGTATCA1000.033.3000031
TTATACA7850.032.993632
CGTCTTC4450.032.84269737
TAGGGCA303.5850838E-430.8333325
ACGCCTG250.005481691629.630
TAGGTAT250.005481691629.63
GCGCCAT250.005481691629.611
GAATATA250.005481691629.61
GCTCTAG250.005481691629.61
CGGATCG250.005481691629.637
GATATAT250.005481691629.621
ACTCGTC250.005481691629.618
TGGATAT250.005481691629.619
AGGGCAA250.005481691629.66
CCGTTTC250.005481691629.611
TATACAC8900.029.1011263
ATCTAAG405.907147E-527.7532
GAGCCCC358.834256E-426.42857213
Produced by FastQC (version 0.11.5)