FastQCFastQC Report
Fri 10 Feb 2017
ERR1630846.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630846.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413256
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG105172.5449116286272915No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT92392.2356602202992817TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT79931.9341521962173567No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC58511.4158294132450586No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC31600.7646591943008693RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC30590.7402191377741641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.588255221944751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22570.5461505701066651No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG13330.3225603500009679No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9640.23326945041330316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8880.21487891282885185No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA8750.21173316297887992No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT8650.20931335540197846No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT8020.19406856766749908No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7150.17301624174845617No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA6500.15728749249859653No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG6140.14857618522175114No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6030.14591439688715954No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5500.13308941672958166No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5420.1311535706680605No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC5120.12389414793735604No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC5010.12123235960276439No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC4950.11978047505662351No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4760.11518284066051067No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT4650.11252105232591905No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4360.10550361035290474No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA4360.10550361035290474No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG4160.10066399519910177No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG4140.10018003368372147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG200.001840572337.02
TGATACA251.2322576E-437.02
GATACAC251.2322576E-437.03
TTGTATA200.001840572337.02
CGTCTTC3950.035.12658337
GCTCTAG507.3086994E-933.3000031
ACAGACA507.3086994E-933.30000317
CAGACAC507.3086994E-933.30000318
AAGTTGT303.5955242E-430.83333419
GATTACG303.5955242E-430.8333341
TTATACA9300.030.4354862
CTTATAC9700.030.3247431
GCAACGC250.005492320729.63
CGCCGTT250.005492320729.625
TTACCGG250.005492320729.631
TACCGGG250.005492320729.632
GGCTCGG454.000094E-628.77777725
AGACAGA604.308822E-827.75000215
CACCAGG405.9310667E-527.7520
GGTATCA3350.027.611941
Produced by FastQC (version 0.11.5)