##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630846.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	413256
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93540081692704	31.0	30.0	33.0	28.0	34.0
2	30.91836537158565	31.0	31.0	34.0	27.0	34.0
3	30.323121745358808	31.0	30.0	34.0	26.0	34.0
4	34.55041911067232	35.0	35.0	37.0	30.0	37.0
5	34.665311574423605	35.0	35.0	37.0	32.0	37.0
6	34.91882029541011	35.0	35.0	37.0	32.0	37.0
7	34.93719873395668	35.0	35.0	37.0	32.0	37.0
8	34.98037778035891	36.0	35.0	37.0	32.0	37.0
9	36.68002158468359	38.0	35.0	39.0	32.0	39.0
10	36.42539249278897	38.0	35.0	39.0	32.0	39.0
11	36.510717327758094	38.0	35.0	39.0	32.0	39.0
12	36.405148382600615	38.0	35.0	39.0	32.0	39.0
13	36.55506514121997	38.0	35.0	39.0	32.0	39.0
14	37.47031138083899	39.0	36.0	40.0	32.0	41.0
15	37.45804053661653	39.0	36.0	40.0	32.0	41.0
16	37.47354182395416	39.0	36.0	40.0	32.0	41.0
17	37.41646098302263	39.0	36.0	40.0	32.0	41.0
18	37.518971291402906	39.0	36.0	40.0	32.0	41.0
19	37.51184737789651	39.0	37.0	40.0	32.0	41.0
20	37.459165747139785	39.0	36.0	40.0	32.0	41.0
21	37.43700272954295	39.0	36.0	40.0	32.0	41.0
22	37.301655632344115	39.0	36.0	40.0	32.0	41.0
23	37.31987194378303	39.0	36.0	40.0	32.0	41.0
24	37.23351385097857	39.0	36.0	40.0	31.0	41.0
25	37.227713572216736	39.0	36.0	40.0	31.0	41.0
26	37.05331804014945	39.0	36.0	40.0	31.0	41.0
27	36.908974098379694	39.0	36.0	40.0	31.0	41.0
28	36.91732485432758	39.0	36.0	40.0	31.0	41.0
29	36.82617312271328	39.0	36.0	40.0	31.0	41.0
30	36.69963412509437	38.0	35.0	40.0	30.0	41.0
31	36.623707822753936	38.0	35.0	40.0	30.0	41.0
32	36.50484445476896	38.0	35.0	40.0	30.0	41.0
33	36.44335230462474	38.0	35.0	40.0	30.0	41.0
34	36.37341502603713	38.0	35.0	40.0	30.0	41.0
35	36.32710232882281	38.0	35.0	40.0	30.0	41.0
36	36.32883007143272	38.0	35.0	40.0	30.0	41.0
37	36.22937114040691	38.0	35.0	40.0	30.0	41.0
38	36.17551348316782	38.0	35.0	40.0	30.0	41.0
39	36.0814095863097	38.0	35.0	40.0	30.0	41.0
40	35.91182221189771	38.0	35.0	40.0	29.0	41.0
41	35.84696410941402	38.0	35.0	40.0	29.0	41.0
42	35.75366358867143	38.0	34.0	40.0	28.0	41.0
43	34.976317343244865	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	3.0
9	4.0
10	10.0
11	15.0
12	14.0
13	13.0
14	10.0
15	9.0
16	14.0
17	4.0
18	23.0
19	38.0
20	81.0
21	158.0
22	336.0
23	590.0
24	979.0
25	1686.0
26	2591.0
27	3873.0
28	5444.0
29	7578.0
30	10365.0
31	13036.0
32	16816.0
33	21774.0
34	28273.0
35	37074.0
36	51196.0
37	72388.0
38	93503.0
39	45353.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.72603906537352	23.98924637512825	16.369514296223166	23.915200263275064
2	14.869717560059625	21.861509572758774	39.88665621309793	23.382116654083667
3	14.664033916022998	31.212855953694564	32.56577036994018	21.55733976034226
4	10.878002981202934	19.584470642894477	37.288024856263426	32.24950151963915
5	11.811806725129218	39.326712739802936	34.99356331184544	13.86791722322241
6	26.132711926747586	40.5801730646379	17.287347310141897	15.999767698472617
7	24.049983545308475	32.99407631105174	22.80087887411193	20.155061269527845
8	25.27537410225139	33.11288886307761	22.779584567435197	18.832152467235804
9	23.249027237354085	14.00149060146737	18.667847532764196	44.08163462841435
10	15.472249646708095	26.561501829374528	33.20072787811913	24.765520645798247
11	30.72284491937201	23.206680604758308	23.972065741332248	22.09840873453743
12	19.902433358499334	28.805389395435277	25.627456104690555	25.664721141374834
13	30.025698356466695	19.452107168437966	25.358131521381424	25.164062953713923
14	22.355634279962057	22.79579727820044	25.28771512089359	29.56085332094392
15	25.507433648876244	28.58058927154113	21.754554077859726	24.157423001722904
16	25.239802930870937	25.24149679617477	22.984784249956444	26.53391602299785
17	20.719360396461273	30.72187699634125	23.23426641113499	25.32449619606249
18	23.151267011247263	24.050225526066168	25.97978008788741	26.818727374799156
19	23.94520587722864	26.79719108736473	27.778423059798286	21.47917997560834
20	22.32369281994696	26.620787115008614	25.708761639274446	25.346758425769984
21	27.21872156726097	24.516522446135085	26.040517258067634	22.224238728536307
22	22.52356892579902	30.191213194726757	25.602532086648473	21.682685792825755
23	23.652167179665874	24.843922411289853	26.436155796891033	25.06775461215324
24	22.022426776622723	30.776564647579224	26.162717540701163	21.03829103509689
25	21.862477495789534	27.895783727278005	28.07993108388021	22.16180769305225
26	26.216195288150683	26.15497415645508	28.246655825928723	19.38217472946551
27	20.990862806589618	31.348849139516428	26.358963935187873	21.30132411870608
28	22.770147317885282	24.20049557659175	29.550206167605552	23.479150937917417
29	24.542172406450238	25.988491395164253	28.064444315388045	21.404891882997465
30	20.900603983971195	25.839431248427125	29.608039568693496	23.651925198908184
31	25.9604216272722	26.086735582786456	25.302717927870376	22.65012486207097
32	23.42204347910254	25.066060746849413	30.242271134599374	21.26962463944867
33	22.088729504229825	24.750517838821455	29.685957372669726	23.474795284278994
34	21.12467816559227	29.62497822173181	27.351085041717482	21.899258570958438
35	21.162669144549625	24.56322473236928	34.63567377122171	19.63843235185938
36	26.135615695839864	25.247788295874713	23.836556517025766	24.780039491259657
37	21.475792245000676	25.030731556226648	29.33363338947287	24.159842809299807
38	21.911841570358327	24.161778655361328	28.247865729717176	25.678514044563176
39	24.74301643533306	24.619606248911087	27.358344464448187	23.27903285130766
40	20.32009214627253	24.109994773215636	34.229630059817644	21.340283020694194
41	22.802572739415762	25.186083202663724	25.78087190506611	26.230472152854407
42	21.339799059178812	24.71930232107943	31.326344929051242	22.614553690690517
43	23.964564337843854	22.911222099618637	30.11232746771977	23.01188609481774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	6.5
2	7.0
3	44.5
4	82.0
5	82.0
6	129.0
7	176.0
8	181.5
9	187.0
10	303.0
11	419.0
12	419.0
13	663.5
14	908.0
15	2236.0
16	3564.0
17	3566.0
18	3568.0
19	3568.0
20	2922.0
21	2276.0
22	1828.0
23	1380.0
24	1733.0
25	2086.0
26	2086.0
27	2841.5
28	3597.0
29	5086.5
30	6576.0
31	8912.0
32	11248.0
33	11248.0
34	14523.0
35	17798.0
36	19954.5
37	22111.0
38	25664.5
39	29218.0
40	29218.0
41	31474.0
42	33730.0
43	35372.0
44	37014.0
45	37913.5
46	38813.0
47	38813.0
48	38510.0
49	38207.0
50	44884.0
51	51561.0
52	39073.0
53	26585.0
54	26585.0
55	33409.5
56	40234.0
57	30913.5
58	21593.0
59	15190.0
60	8787.0
61	8787.0
62	7396.5
63	6006.0
64	4398.0
65	2790.0
66	2146.0
67	1502.0
68	1502.0
69	1100.0
70	698.0
71	511.5
72	325.0
73	211.0
74	97.0
75	97.0
76	72.0
77	47.0
78	33.5
79	20.0
80	14.0
81	8.0
82	8.0
83	8.5
84	9.0
85	9.5
86	10.0
87	9.5
88	9.0
89	9.0
90	5.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	413256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.887808041504538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.11466273781308	13.243122906866445
2	9.686759336365384	3.6592330177904255
3	4.307219268464544	2.4406179220628377
4	2.487989238357568	1.8797065257370733
5	1.7500480430465697	1.6527285750237142
6	1.2811479085260393	1.451884546140891
7	1.0607904682595606	1.4025204715721007
8	0.8032797386458266	1.2137754805737848
9	0.6162321440010249	1.0475347000406527
>10	6.24303375824739	24.90683740828929
>50	1.0197937351867274	13.423398571345608
>100	0.6008583690987125	20.38881468145653
>500	0.016654922810838513	2.1974272605842384
>1k	0.006405739542630197	2.9618444741274175
>5k	0.003843443725578118	5.585641829761697
>10k+	0.0012811479085260394	2.5449116286272915
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	10517	2.5449116286272915	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	9239	2.2356602202992817	TruSeq Adapter, Index 1 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	7993	1.9341521962173567	No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	5851	1.4158294132450586	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3160	0.7646591943008693	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3059	0.7402191377741641	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.588255221944751	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2257	0.5461505701066651	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1333	0.3225603500009679	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	964	0.23326945041330316	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	888	0.21487891282885185	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	875	0.21173316297887992	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	865	0.20931335540197846	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	802	0.19406856766749908	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	715	0.17301624174845617	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	650	0.15728749249859653	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	614	0.14857618522175114	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	603	0.14591439688715954	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	550	0.13308941672958166	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	542	0.1311535706680605	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	512	0.12389414793735604	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	501	0.12123235960276439	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	495	0.11978047505662351	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	476	0.11518284066051067	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	465	0.11252105232591905	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	436	0.10550361035290474	No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGA	436	0.10550361035290474	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	416	0.10066399519910177	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTG	414	0.10018003368372147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.4198075769014848E-4	0.0
16	0.0	0.0	0.0	2.4198075769014848E-4	0.0
17	0.0	0.0	0.0	2.4198075769014848E-4	0.0
18	0.0	0.0	0.0	2.4198075769014848E-4	0.0
19	0.0	0.0	0.0	2.4198075769014848E-4	0.0
20	0.0	0.0	0.0	2.4198075769014848E-4	0.0
21	0.0	0.0	0.0	0.0014518845461408908	0.0
22	0.0	0.0	0.0	0.003387730607662079	0.0
23	0.0	0.0	0.0	0.0038716921230423757	0.0
24	0.0	0.0	0.0	0.0043556536384226725	0.0
25	0.0	0.0	0.0	0.00483961515380297	0.0
26	0.0	0.0	0.0	0.005323576669183267	0.0
27	0.0	0.0	0.0	0.006533480457634009	0.0
28	0.0	0.0	0.0	0.011615076369127127	0.0
29	0.0	0.0	0.0	0.022988171980564104	0.0
30	0.0	0.0	0.0	0.040894748049635096	0.0
31	0.0	0.0	0.0	0.07864374624929825	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAACG	20	0.0018405723	37.0	2
TGATACA	25	1.2322576E-4	37.0	2
GATACAC	25	1.2322576E-4	37.0	3
TTGTATA	20	0.0018405723	37.0	2
CGTCTTC	395	0.0	35.126583	37
GCTCTAG	50	7.3086994E-9	33.300003	1
ACAGACA	50	7.3086994E-9	33.300003	17
CAGACAC	50	7.3086994E-9	33.300003	18
AAGTTGT	30	3.5955242E-4	30.833334	19
GATTACG	30	3.5955242E-4	30.833334	1
TTATACA	930	0.0	30.435486	2
CTTATAC	970	0.0	30.324743	1
GCAACGC	25	0.0054923207	29.6	3
CGCCGTT	25	0.0054923207	29.6	25
TTACCGG	25	0.0054923207	29.6	31
TACCGGG	25	0.0054923207	29.6	32
GGCTCGG	45	4.000094E-6	28.777777	25
AGACAGA	60	4.308822E-8	27.750002	15
CACCAGG	40	5.9310667E-5	27.75	20
GGTATCA	335	0.0	27.61194	1
>>END_MODULE