FastQCFastQC Report
Fri 10 Feb 2017
ERR1630845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443984
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT78151.7601985657140797No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG78131.7597480990305958No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT56961.2829291145626869Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC42810.9642239359976936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23800.5360553533460665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22540.5076759522865689No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC20890.4705124508991315No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC18450.4155555155140726Illumina PCR Primer Index 11 (95% over 23bp)
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG13870.3123986449962161No Hit
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG11090.24978377599192766No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9640.21712494143933114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8160.18379040686150852No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA7890.1777091066344733No Hit
TTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTA6890.15518577246026885No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG6680.1504558722836859No Hit
ACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT5800.13063533821038595No Hit
TATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTAT5710.12860823813470754No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC5520.12432880464160871No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5240.11802227107283146No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG5090.11464377094670078No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5080.11441853760495874No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT4910.11058957079534398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGG452.6120688E-937.03
CGTCTTC2950.033.86440737
GATTACG451.0774238E-732.888891
CTTATAC9550.032.1570661
GCCTACC303.5959514E-430.8333341
TATACAC10750.029.2558163
TTATACA10900.028.853212
GGTATCA3050.027.901641
GTATCAA9700.025.365981
CCGTCTT9200.024.33152237
GCCGTCT9650.023.19689236
ATTACCC802.7162969E-823.1253
CGCAAGT400.001929330523.12512
ATTACGG753.7300742E-722.22
CGGAGTT502.6984836E-422.224
ATTACCG603.7196583E-521.5833345
GCACGGA603.7196583E-521.58333421
TTACCGC603.7196583E-521.5833346
ATAGCGG705.0902927E-621.1428577
TCGGGAT705.0902927E-621.14285722
Produced by FastQC (version 0.11.5)